Session: 637. Myelodysplastic Syndromes—Clinical Studies: Personalized Clinical-Decision Tools and treatment of lower risk MDS
Hematology Disease Topics & Pathways:
Methods: Targeted DNAm was quantified using bisulfite padlock probes (BSPP) at ~141,000 unique regions known to contain differentially methylated CpG sites. BSPP sequencing was performed on 130 genetically and clinically annotated bone marrow mononuclear cell DNA samples collected at screening and Cycle 2 Day 22±3 (C2D22). Data from 15 samples were removed due to low CpG coverage resulting in 115 samples with >10X coverage of 322,387 CpGs each. There were 27 patients with paired samples, 30 with screening only, and 31 with C2D22 only (Table 1, Figure 1A). Responses for HR-MDS and CMML were categorized according to the IWG-2006 modified criteria and for LB-AML according to the IWG-2003 criteria. Differentially methylated CpGs were identified using the R package ‘DSS’ v2.35.0. Non-negative matrix factorization-based unsupervised clustering was performed using the Onco-GPS method within the Python ‘ccal’ package. Statistical analyses were carried out using R version 3.6.2.
Results: Differential DNAm analysis between screening and C2D22 for the 27 paired samples shows global demethylation after treatment in nearly all cases but is more pronounced in responders (mean change in global DNAm: response = -5.5%; non-response = -2.0%; t-test p < 0.001). There was no significant difference in demethylation between AZA and AZA+PEV treated groups (AZA: -5.3%; AZA+PEV: -4.1%; t-test p = 0.32). Patients who attained a complete response (CR) in the AZA+PEV arm tended to have more demethylated CpGs than those with a CR in the AZA arm (Figure 1B. Mean number of demethylated CpGs in complete responders: AZA = 16,459; AZA+PEV = 24,731; t-test p = 0.396). In general, responding patients strictly defined by CR or partial response had steeper declines in mean DNAm levels (Figure 1C) than patients with other types of responses, but did not show an association between demethylation and time to response (TTR). Unsupervised clustering of the top 3% most variably methylated CpGs using Onco-GPS did not identify clusters associated with response at screening, but did resolve a cluster highly enriched for responders assessed at C2D22 (Figure 1D. Cluster B, Fisher’s exact test p = 0.011; odds-ratio 95% CI = 1.52–Inf). Analysis of the CpG sites that define Cluster B and creation of a formalized statistical predictor of response based on DNAm patterns is ongoing.
Conclusions: DNAm patterns early in the course of HMA treatment correlate with eventual response, with CRs associated with the greatest degree of demethylation. Responders are more likely to have greater demethylation that may reflect greater effective AZA exposure or slower remethylation dynamics. Addition of PEV may intensify site-specific demethylation in responders and increase the proportion of responding patients. With a median TTR of 3-4 months in the P-2001 study, depth of demethylation at the C2D22 timepoint may help predict eventual response. Strategies to augment demethylation with adjunctive agents or HMA dose escalation in patients with early inadequate demethylation should be investigated. Ongoing work will incorporate mutation status and focus on refining DNAm signatures that can predict eventual response.
Disclosures: Luger: Arcturus Therapeutics: Current Employment. Zhao: Millennium Pharmaceuticals Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited: Current Employment. Fram: Takeda, Bristol Myers, Gilead, Pfizer, Baxter, Teva: Current equity holder in publicly-traded company; BeyondSpring Pharmaceuticals Inc.: Consultancy; Takeda Pharmaceuticals Intl. Co.: Current Employment; Vertex Pharmaceuticals: Patents & Royalties: No Royalties; Patent 10/728,114 (Vertex Pharmaceuticals). Dash: Millennium Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited: Current Employment, Other: Stockholder. Bejar: Forty-Seven/Gilead: Honoraria; Genoptix/NeoGenomics: Honoraria; Daiichi-Sankyo: Honoraria; Takeda: Honoraria, Research Funding; Celgene/BMS: Honoraria, Research Funding; Astex/Otsuka: Honoraria; AbbVie/Genentech: Honoraria; Aptose Biosciences: Current Employment.
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