Session: 618. Acute Myeloid Leukemias: Biomarkers and Molecular Markers in Diagnosis and Prognosis: Poster II
Hematology Disease Topics & Pathways:
Research, Acute Myeloid Malignancies, AML, Translational Research, Drug development, Bioinformatics, Diseases, Treatment Considerations, Computational biology, Myeloid Malignancies, Technology and Procedures, Omics technologies
To bridge this gap, we introduce ATLAS-AML, an automated bioinformatics pipeline for transcriptomic meta-analysis of genes and gene signatures in adult and pediatric AML. ATLAS-AML integrates 30 bulk (2172 donors) and single-cell (975,220 cells from 283 donors) RNA-sequencing datasets with preconfigured pipelines to streamline three key analyses for genes or gene signatures: (1) expression across normal and leukemic hematopoietic hierarchies, (2) enrichment in functionally-validated LSC+ fractions and (3) associations with relapse, genomics, clinical characteristics and patient survival. For established targets, ATLAS-AML can determine which patients, based on their mutational and cytogenetic profiles, are most likely to respond favorably to a potential treatment. ATLAS-AML is available as a containerized R package that experimental scientists can employ without bioinformatics expertise.
To demonstrate how ATLAS-AML can guide target prioritization and characterization, we systematically analyzed published targets in ATLAS-AML. We identified 70 targets reported in the literature to be either enriched in leukemia, associated with LSCs, overexpressed at relapse, or predictive of patient survival. After benchmarking each gene against the aforementioned outcomes in ATLAS-AML, we observed that published targets were often optimized for particular outcomes, potentially overlooking other critical perspectives of AML biology. For example, several genes were enriched on leukemia cells, but not associated with genomics and stemness perspectives of AML heterogeneity nor clinical outcomes like relapse and patient survival.
ATLAS-AML also constitutes a powerful framework for accelerating new target discovery. Reinterrogating our dataset with differential expression, we identified genes with enrichment in the same outcomes we benchmarked published targets against. Applying a meta p-value analysis, ATLAS-AML uncovered 13 targets that were overexpressed on leukemia cells compared to normal hematopoietic cells, enriched in functionally-validated LSCs, associated with disease relapse, and predictive of patient survival. For example, ATLAS-AML nominated CNST, a trans-Golgi network receptor for targeting connexins to the plasma membrane. CNST is differentially expressed on leukemia cells (p=0.000043), linked to functional LSC engraftment (p=0.000015), overexpressed at relapse (p=0.00036) and associated with poor prognosis in multivariable survival analysis in three independent cohorts (HR 1.23, p=0.04), lending to CNST’s therapeutic viability in AML. Furthermore, ATLAS-AML determined that CNST expression was highest in RUNX1-mutated AMLs, identifying a patient group to prioritize for anti-CNST therapies.
Altogether, ATLAS-AML enables scientists to leverage insights from single-cell and bulk transcriptomics to inform preclinical studies towards risk-tailored treatments in AML.
Disclosures: Dick: Pfizer: Patents & Royalties; Celgene/BMS: Research Funding.