Session: 622. Lymphomas: Translational – Non-Genetic: Poster I
Hematology Disease Topics & Pathways:
Research, Translational Research
Methods: We employed imaging mass cytometry with a panel of 36 meta-tagged antibodies to examine the immune architecture of lymph nodes from 13 FL patients, both at diagnosis and during POD24.
Results: Our analysis revealed a decrease in immune infiltration from the peri-follicular regions towards the follicular centers in FL. Notably, there was an increase in CD163+ macrophages within the follicles, accompanied by an upregulation of PD-1 ligands during POD24. Spatial analysis showed that FL cells tend to evade immune attacks not merely through spatial separation from CD8+ T cells but by proactively recruiting immunosuppressive cells prior to encountering these T cells. Furthermore, during POD24, more CD8+ T cells in proximity to FL cells were found interacting with macrophages rather than regulatory T cells (Tregs). There was also an observed increase in the cooperation between macrophages and Tregs, which collectively worked to subdue cytotoxic T cell activity, thereby creating a more immunosuppressed environment.
Conclusions: FL cells exhibit more aggressive evasion tactics and exist within a more immunosuppressive microenvironment during POD24. These findings suggest that immunotherapeutic strategies targeting PD-1, macrophages, and CTLA-4 may hold promise for improving outcomes in FL patients experiencing early progression.
Disclosures: No relevant conflicts of interest to declare.
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