Session: 803. Emerging Tools, Techniques, and Artificial Intelligence in Hematology: Poster I
Hematology Disease Topics & Pathways:
Research, Artificial intelligence (AI), Translational Research, Genetic Disorders, Plasma Cell Disorders, Diseases, Lymphoid Malignancies, Technology and Procedures, Imaging, Machine learning
BMA samples from non MM patients, MM patients without the targeted genetic alterations, and MM patients with confirmed alterations (t(11;14), +1q, del17p, del1p) were analyzed by digitizing 40 fields (100x) per patient using a 3D-printed device that attaches a mobile application to an optical microscope. Firstly, an AI algorithm was developed using a database of over 400,000 manually labeled cells, enabling automatic identification of nucleated cells and classification into plasma cells and non-plasma cells. Subsequently, another AI algorithm was designed to recognize morphological patterns of PC indicative of the genetic alterations. This algorithm is based on a Multiple Instance Learning (MIL) architecture, utilizing features extracted from each cell by a foundational model trained through self-supervised learning on a database of over 1.4 million BMA cells. The dataset was divided into 66% for training and 33% for testing, ensuring patient-level independence between the sets.
A total of 946 digitized cases were analyzed, comprising 250 MM patients and 696 non-MM patients. Among the MM patients, 109 did not have the targeted genetic alterations, while 141 MM had specific genetic alterations. Of these, 80 had t(11;14), 64 had +1q, 24 had del17p, and 28 had del1p, with some patients presenting multiple alterations. The AI algorithm for detecting plasma cells achieved a ROC-AUC of 98%. On average, 174 plasma cells were detected and analyzed per BMA sample. The AI algorithm designed to identify patients with these genetic alterations based on morphological analysis of plasma cells demonstrated high efficacy. For t(11;14), the algorithm achieved an AUC of 92.50%, with a sensitivity of 91.67% and a specificity of 92,20%. For +1q, the AUC was 96.00%, with a sensitivity of 89.47% and a specificity of 93.94%. For del1p, the AUC was 96.80%, with a sensitivity of 100.00% and a specificity of 96.17%. For del17p, the AUC was 86.00%, with a sensitivity of 100.00% and a specificity of 80.39%.
Our results underscore the potential of applying AI models to the morphological analysis of plasma cells in BMA samples for the detection of multiple genetic alterations, confirming a significant correlation between cell morphology and genetics in MM cases. The developed AI algorithm accurately identifies the presence of morphological features suggestive of t(11;14), +1q, del17p, and del1p with high sensitivity and varying specificity. Clinically, this AI-driven approach could simplify the diagnosis and prognosis of MM patients, enabling more targeted therapeutic interventions. Overall, the algorithm demonstrated high sensitivity across all alterations, with specificity varying by alteration. The exceptionally high sensitivity and specificity for del1p, and the high sensitivity for del17p, in particular, highlight the potential of AI in accurately identifying these clinically significant genetic alterations based on morphology. Improved performance for other alterations may be achieved through increasing sample sizes, refining model architectures, or incorporating additional predictive variables. This advancement holds promise for integrating AI tools into routine clinical practice, potentially reducing the need for extensive genetic testing and accelerating the decision-making process for personalized MM treatment strategies.
Disclosures: Bermejo-Peláez: Spotlab: Current Employment. Díez Navarro: Spotlab: Current Employment. Brau Queralt: Spotlab: Current Employment. Postigo Camps: Spotlab: Current Employment. Lin: Spotlab: Current Employment. Mancebo-Martín: Spotlab: Current Employment. Caballero Moreno: Spotlab: Current Employment. Angulo Llorente: Spotlab: Current Employment. Vladimirov: Spotlab: Current Employment. Ledesma-Carbayo: Spotlab SL: Other: shareholder and member . Luengo-Oroz: Spotlab: Current Employment. Martínez-Lopez: Janssen: Consultancy, Honoraria, Research Funding; Amgen: Consultancy, Honoraria; Roche: Consultancy, Honoraria; Pfizer: Consultancy, Honoraria, Research Funding; BMS: Consultancy, Honoraria; Kite: Consultancy, Honoraria; Incity: Research Funding.