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1351 Parallel Sequencing of Transposons and Transcriptomes in Single Cells for Genome-Wide Cancer Gene Discovery

Program: Oral and Poster Abstracts
Session: 602. Myeloid Oncogenesis: Basic: Poster I
Hematology Disease Topics & Pathways:
Research, Fundamental Science, Acute Myeloid Malignancies, AML, Translational Research, Genomics, Bioinformatics, Diseases, Computational biology, Myeloid Malignancies, Biological Processes, Emerging technologies, Molecular biology, Technology and Procedures, Omics technologies
Saturday, December 7, 2024, 5:30 PM-7:30 PM

Maximilian Holz1,2*, Bertram Klinger3,4*, Felix Stuckenbrok1,5,6*, Stefanos A. Bamopoulos, MD7,8*, Sanket Gosavi5*, Caroline Bräuning9*, Janine Altmüller9*, Roland Rad10*, Torsten Haferlach, MD6, Nils Blüthgen11,12*, Ulrich Keller13,14,15,16*, Thomas Conrad17* and Francis Baumgartner1,5,6,12*

1Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
2Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charité, Berlin, DEU
3Institute of Pathology, Charité-Universitätsmedizin Berlin, Berlin, Germany
4Institute for Theoretical Biology, Humboldt-Universität zu Berlin, Berlin, Germany
5Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin, Berlin, Germany
6MLL Munich Leukemia Laboratory, Munich, Germany
7Max-Delbrück-Center for Molecular Medicine,, Berlin, Germany
8Department of Hematology, Oncology and Tumor Immunology (Campus Benjamin Franklin), Charité University Medicine Berlin, Berlin, DEU
9Genomics Technology Platform, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
10Institute of Molecular Oncology and Functional Genomics, Technical University of Munich, Munich, Germany
11Institute of Pathology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany
12German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Berlin, Germany
13German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany
14Max-Delbrück-Center, Berlin, Germany
15Hematology, Oncology and Cancer Immunology, Charité - University Medical Center Berlin, Campus Benjamin Franklin, Berlin, Germany
16German Cancer Consortium (DKTK) partner site Berlin, a partnership between DKFZ and Charité – Universitätsmedizin Berlin, Berlin, Germany
17Genomics Technology Platform, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, DEU

Introduction

Genome-wide transposon mutagenesis screens in mice are powerful tools for identifying genomically, transcriptionally, and epigenetically deregulated cancer genes in a single experimental approach. A major limitation, however, is the need for large cohort sizes and substantial sequencing and bioinformatic resources to differentiate causal genetic changes from silent transposon insertions. We address these challenges with single cell PiggyBac Transposon and Transcriptome Sequencing (scPB&T-seq), a novel dual-assay that enables the identification of disrupted molecular networks at the single cell level, thus improving precision and efficacy of cancer gene studies.

Methods

A murine PiggyBac (PB) transposition screen was performed to generate Asxl1-mutated (Asxl1mut) acute myeloid leukemias (AMLs), a subgroup with particularly poor prognosis. For scPB&T-seq, single cells (sc) from three AMLs were isolated, and genomic DNA was physically separated from mRNA. Sc transposon insertions were amplified via multiple displacement followed by a custom PCR amplification and sequenced alongside sc transcriptomes derived by Smart-Seq2. The data was then integrated with high-throughput droplet-based scRNAseq from the same samples.

Results

Activating transposon mutagenesis in an Asxl1mut background led to the identification of a large candidate gene catalogue for Asxl1mut AML pathogenesis. Using scPB&T-seq we identified an average of 10-25 transposons per cell, aligning with expected frequencies. All insertions were confirmed in concurrent bulk insertion analyses. About 60% of the insertions impacted gene expression and were retained for further analysis. By integrating scRNAseq data, the insertions were assigned to specific cellular clusters, allowing the reconstruction of clonal evolution and identification of intratumoral heterogeneity. This approach confirmed known AML drivers co-occurring with ASXL1 mutations, such as PRDM16 and MECOM, and revealed novel genomic loci linked to AML pathogenesis. These findings were validated in whole genome and whole transcriptome sequencing data from 774 human AML patients generated within the Munich Leukemia Laboratory 5K Genomes Project. Interestingly, 45 (5.9%) patients from this dataset exhibited a transcriptional profile similar to the main clone of an Asxl1mut PB-AML, showcasing an upregulation of e.g., DOCK5, TCF4, and CSF1. Furthermore, this subgroup of patients displayed a significant enrichment for ASXL1 (31.1% vs. 14.5%, P = 0.005), NRAS (28.9% vs. 15.6%, P = 0.035) and concurrent ASXL1 and NRAS (13.3% vs. 3%, P = 0.004) mutations compared to the remaining 724 AML patients. Of note, an insertion in Nras was an activating insertion in a monocytic-like subclone of this Asxl1mut PB-AML.

Conclusion

In this study we developed scPB&T-seq, an innovative method to analyze transposon screens that uncovers functional mechanisms behind individual transposon insertions in single cells of heterogenous tumors. This enables the assessment of clonal evolution and oncogenic networks in unprecedented detail, thereby enhancing our understanding of AML pathogenesis and potentially guiding the development of targeted therapies.

Disclosures: No relevant conflicts of interest to declare.

*signifies non-member of ASH