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309 A Reduced Daunorubicin Dose during Induction Is Equally Effective As Standard Dose in Children with Acute Lymphoblastic Leukemia (ALL) and Favorable Prognostic Features: Long-Term Results of the Phase 3 Trial AIEOP-BFM ALL 2009

Program: Oral and Poster Abstracts
Type: Oral
Session: 613. Acute Lymphoblastic Leukemias: Therapies Excluding Allogeneic Transplantation: Clinical Trials in Pediatric and Young Adult Patients
Hematology Disease Topics & Pathways:
Lymphoid Leukemias, ALL, Combination therapy, Diseases, Treatment Considerations, Lymphoid Malignancies, Adverse Events, Measurable Residual Disease
Saturday, December 7, 2024: 4:30 PM

Martin Schrappe, MD1,2, Hanna Marie Gottschalk, MD3*, Valentino Conter, MD4*, Gunnar Cario, MD1,5*, Jan Stary, MD, DSc6,7*, Anja Möricke, MD5*, Michael Dworzak, MD8,9*, Andishe Attarbaschi, MD10,11*, Draga Barbaric, MD12,13*, Franco Locatelli, MD14,15, Nicole Bodmer, MD16*, Sarah Elitzur, MD17,18, Daniela Silvestri4*, Luciano Dalla-Pozza, MD19, Bernd Gruhn20,21*, Shai Izraeli, MD17,22, Carmelo Rizzari, MD, PhD23*, Jean-Pierre Bourquin, MD, PhD24, Andrea Biondi25, Maria Grazia Valsecchi, PhD25* and Martin Zimmermann, PhD26*

1Clinical Research Unit CATCH ALL (KFO 5010/1) funded by the Deutsche Forschungsgemeinschaft, Kiel, Germany
2Pediatric Hematology/Oncology, ALL-BFM Study Group, Christian-Albrechts-University, Medical Faculty, Kiel, Kiel, Germany
3Pediatrics, ALL-BFM Study Group, University Medical Center Schleswig-Holstein, Campus Kiel, Kiel, Germany
4Tettamanti Center, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy
5Department of Pediatrics I, Pediatric Hematology and Oncology, ALL-BFM Study Group, University Medical Center Schleswig-Holstein, Campus Kiel, Kiel, Germany
6Charles University and University Hospital Motol, CLIP - Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Prague, Czech Republic
7Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic
8St. Anna Children's Cancer Research Institute, Vienna, Austria
9Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, St. Anna Children's Hospital, Vienna, Austria
10Department of Pediatric Hematology and Oncology, St. Anna Kinderspital, Vienna, Austria
11St. Anna Children´s Cancer Research Institute (CCRI), Vienna, Austria
12Kids Cancer Centre, Sydney Children's Hospital, School of Clinical Medicine, UNSW Sydney, Sydney, Sydney, Australia
13Centre for Children's Cancer and Blood Disorders, Sydney Children's Hospital, Randwick NSW, AUS
14IRCCS Ospedale Pediatrico Bambino Gesù, Catholic University of the Sacred Heart, Rome, Italy
15Department of Pediatric Hematology/Oncology, Cell and Gene Therapy, IRCCS Bambino Gesù Children’s Hospital, Catholic University of the Sacred Heart, Rome, Italy
16Department of Oncology, University Children's Hospital Zurich, Zurich, Switzerland
17Department of Pediatric Hematology and Oncology, Schneider Children's Medical Center and Tel Aviv University, Petah Tikva, Israel
18Schneider Children's Medical Center, Petah Tikva, Israel
19Cancer Centre for Children, Sydney, Australia
20Department of Pediatrics, Jena University Hospital, Jena, Germany
21Department of Pediatrics, Department of Pediatrics, Jena, Germany
22Pediatric Hematology-Oncology, Sheba Medical Center, Petach Tikva, Israel
23Pediatrics, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy
24Department of Pediatric Oncology, University Children's Hospital Zurich, Zurich, Switzerland
25School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy
26Department of Pediatric Hematology and Oncology, Medical School Hannover, Hannover, Germany

Background: Anthracyclines have contributed to the development of effective antileukemic treatment of ALL. Modern ALL therapy, however, is shifting its attention towards reduction of acute and late toxicities while maintaining the same cure rate. Acute and late sequelae of anthracyclines are of major concern, thus, this trial aimed to clarify their role in low-risk patients (pts).

Patients and Methods: The randomization R1 was included in trial AIEOP-BFM ALL 2009 for pediatric pts (1 to < 18 years of age) with newly diagnosed ALL with the aim to safely reduce the daunorubicin (DNR) dose in induction with non-inferior event-free survival (EFS) and reduced toxicity. Pts were eligible for R1 if they had non-high-risk B-ALL (i.e. good response to the prednisone pre-phase, absence of hypodiploidy or KMT2A::AFF1 rearrangement) with either positivity for ETV6::RUNX1 or rapid response to the first two weeks of induction treatment (flow cytometry d15 with < 0.1% marrow blasts). After two weeks of induction therapy including 2 DNR doses (30 mg/m2 on days 8 and 15), pts were randomly assigned to receive either a 3rd and 4th DNR dose (on days 22 and 29, control arm, CA) or no further DNR during induction (experimental arm, EA). Other treatment components have been previously described (Rizzari C et al, Hemasphere 2023, 7:e893). Depending on minimal residual disease (MRD, evaluated by real-time quantitative PCR) at the end of induction (EoI) and consolidation (EoC), pts were finally stratified into standard risk (SR), medium risk (MR), or high risk (HR) (Conter V. et al, J Clin Oncol 2023, 42:915-926). Pts who were treated as assigned by randomization R1 were included in the primary analyses on EFS, cumulative incidence of relapse (CIR), and overall survival (OS). Secondary outcome analyses were done by intention to treat (ITT). Incidences of protocol-defined adverse reactions (AR) of special interest, occurring during the randomized treatment phase (from day 22 of induction to start of consolidation) were analyzed in the as-treated population.

Results: Out of 6136 pts enrolled in AIEOP-BFM ALL 2009, 2514 pts (41.0 %) were eligible for R1. Of those, 2079 pts (82.7 %) were randomized to receive either the EA (n=1040) or the CA (n=1039). Forty randomized pts were not included in the treated-as-assigned population because they were either retrospectively not eligible (n=5), died before day 22 (n=1) or did not receive the assigned arm (switch from CA to EA (n=13) or vice versa (n=19) or 3rd DNR dose given by mistake in EA (n=2)) resulting in 1016 pts in EA and 1023 pts in CA. Patient characteristics were equally distributed between the arms. With a median observation time of 7.0 years, the probability of 5-year EFS in the treated-as-assigned population was 92.7% (standard error, SE 0.8%) in CA and 92.2% (SE 0.9%) in EA. The lower limit of the one-sided 95% confidence interval for the difference was -2.3%, well above the pre-defined non-inferiority margin of -4%. CIR at 5 years was 5.6% (SE 0.7%) in CA compared to 5.9% (SE 0.8%) in EA. Five-year OS was 97.4% (SE 0.5%) in CA and 97.7% (SE 0.5%) in EA. Additional analyses in various subgroups (gender, age, ETV6::RUNX1 status, MRD status at EoI or at EoC, final risk groups SR, MR, or HR) did not reveal any differences in outcome between the randomization arms. Outcome results of ITT analyses were almost identical: 5y-EFS 92.7% (SE 0.8%) in CA, and 92.3% (SE 0.8%) in EA. AR of special interest were observed in 7.4% and 6.1% of pts in CA and EA, respectively (p=0.25). Life-threatening AR occurred in 1.4% in CA and 1.1% in EA (p=0.45), and were fatal in 0.7% and 0.5% of pts, respectively. Analyses of AR types revealed a trend towards a higher incidence of infection-related AR in CA than in EA (2.9% vs 1.7%, p=0.061) due to a four times higher incidence of invasive fungal infections (IFI) in CA than in EA (1.5% vs 0.4%, p=0.0076).

Conclusion: The results of this unique prospective trial in a large patient cohort clearly show that a 50% reduction of the cumulative DNR dose during induction did not compromise the outcome. Although the two different schedules showed almost no difference in the overall incidence of serious toxicity, the risk of IFI could be significantly mitigated by reducing the DNR dose. Our study demonstrates, that one can safely recommend reduced anthracyclines in ALL induction therapy if patients are selected by favorable prognostic factors, such as presence of ETV6::RUNX1, or very fast early treatment response.

Disclosures: Schrappe: JazzPharma, Servier, Amgen: Honoraria, Research Funding, Speakers Bureau. Cario: Jazz Pharmaceuticals: Other: travel support. Dalla-Pozza: Amgen: Honoraria, Membership on an entity's Board of Directors or advisory committees; JazzPharmaceuticals: Honoraria, Membership on an entity's Board of Directors or advisory committees. Rizzari: AMGEN, CLINIGEN, JAZZ, SERVIER, SERB: Consultancy, Honoraria, Speakers Bureau. Biondi: CoImmune, Galapagos, Amgen, Novartis, BMS: Consultancy, Research Funding, Speakers Bureau.

*signifies non-member of ASH