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1928 Tumor-Intrinsic and Immune Predictors of Response to Ixazomib, Lenalidomide, and Dexamethasone in High-Risk Smoldering Multiple Myeloma

Program: Oral and Poster Abstracts
Session: 653. Multiple Myeloma: Clinical and Epidemiological: Poster I
Hematology Disease Topics & Pathways:
Research, Clinical trials, Translational Research, Clinical Research, Plasma Cell Disorders, Diseases, Immune mechanism, Immunology, Lymphoid Malignancies, Biological Processes, Molecular biology
Saturday, December 7, 2024, 5:30 PM-7:30 PM

Michelle P. Aranha, PhD1,2,3*, Omar Nadeem, MD4, Robert A. Redd, MS5*, Michael Timonian, MD1,2,3*, Sophie Magidson, BS2*, Elizabeth D. Lightbody, PhD1,2,3, Jean-Baptiste Alberge, PhD1,2,3, Ting Wu, MS1*, Junko Tsuji, PhD1*, Luca Bertamini, MD2*, Ankit K. Dutta, PhD1,2,3*, Habib El-Khoury, MD2*, Mark Bustoros, MD6, Jacob Laubach2, Giada Bianchi, MD7, Elizabeth K. O'Donnell, MD2, Kenneth C. Anderson, MD2,3, Gad Getz, PhD1*, Lorenzo Trippa, PhD5*, Paul G. Richardson2,3, Romanos Sklavenitis-Pistofidis, MD1,2,3 and Irene Ghobrial, MD1,2,3

1Broad Institute of MIT and Harvard, Cambridge, MA
2Dana-Farber Cancer Institute, Boston, MA
3Harvard Medical School, Boston, MA
4Center for Early Detection and Interception of Blood Cancers, Dana-Farber Cancer Institute, Boston, MA
5Department of Data Science, Dana-Farber Cancer Institute, Boston, MA
6Division of Hematology & Medical Oncology, Weill Cornell Medicine/New York Presbyterian Hospital, New York, NY
7Amyloidosis Program, Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

Background

Early treatment of patients with high-risk smoldering myeloma (HRSMM) has been shown to delay progression to multiple myeloma (MM), but not all patients respond well. Identifying biological predictors of response and resistance to treatment can help optimize treatment selection for patients and improve outcomes. Here, we identify tumor and immune biomarkers of response to ixazomib, lenalidomide, and dexamethasone treatment (I-PRISM study) that can be measured at diagnosis.

Methods

We performed single-cell RNA sequencing (scRNAseq), coupled with B cell receptor and T cell receptor sequencing, on CD138+ tumor and CD138- immune cells as well as whole genome sequencing (WGS) on tumor cells from the bone marrow of 22 HRSMM patients (14 biochemical progressors, 8 non-progressors) enrolled in the I-PRISM study and 11 healthy donors. Among progressors, 5 patients went on to develop myeloma by the SLiM-CRAB criteria.

Results

The median time to biochemical progression was 33 months and the median follow-up for non-progressors was 48 months. 35 patients were successfully cytogenetically classified by FISH; in the remaining patients (n=10), we inferred the presence of IgH translocations and copy number variants (CNVs) using scRNAseq and WGS. Translocations and CNVs inferred from scRNAseq showed excellent concordance with WGS data, suggesting that scRNAseq-based cytogenetic classification is reliable. Notably, scRNAseq identified additional CNVs (n=7) that were missed by FISH, highlighting its greater sensitivity. No significant association was observed between cytogenetics and response to treatment.

Differential expression analysis of tumor cells identified by BCRseq revealed higher MHC-I gene expression in non-progressors compared to progressors, suggesting a potential link between MHC-I expression and response to therapy. We validated this observation using data from the PADIMAC study, where MM patients were treated with a different proteasome inhibitor (PI; Bortezomib) in a combination that did not include lenalidomide. This suggests a broader link between tumor-intrinsic MHC-I expression and response to proteasome inhibition (PI). Gene set enrichment analysis (GSEA) further revealed upregulated oxidative phosphorylation pathways in tumor cells from progressors, suggesting enhanced energy metabolism as a driver of progression post-treatment with PIs. Since cytotoxic CD8+ T cells recognize and eliminate cells presenting endogenous antigens on their surface via the MHC-I pathway, we investigated whether they show differences between progressors and non-progressors. By comparing the clonal expansion rate of two CD8+ T cell subsets, the earlier, memory-like granzyme GZMK+ compartment and the more terminally differentiated GZMB+ compartment, we found that progressors exhibited greater clonal expansion in the GZMK+ compartment than non-progressors. Notably, we previously showed that the GZMK+ compartment is the main source of PD-1 expression, a key T cell exhaustion marker, in patient bone marrow. Additionally, clonally expanded T cells from progressors showed a reduced proportion of the GZMB+ phenotype, suggesting their clonally expanded T cells may have a less mature cytotoxic profile compared to non-progressors. This may be related to the lower levels of MHC-I expression observed in tumor cells from progressors, which may indicate impaired tumor antigen presentation and cytotoxic T cell activation.

Conclusions

Our results highlight the role of the immune microenvironment and its complex interplay with tumor cells, potentially involving tumor antigen presentation and cytotoxic T cell activation, in response to PI-based regimens. Immune profiling may help to improve risk stratification of patients with HRSMM and MM and inform the selection of therapy for specific patients.

Disclosures: Nadeem: BMS: Membership on an entity's Board of Directors or advisory committees, Research Funding; Pfizer: Honoraria; JNJ: Research Funding; GPCR Therapeutics: Membership on an entity's Board of Directors or advisory committees; Sanofi: Membership on an entity's Board of Directors or advisory committees; Janssen: Membership on an entity's Board of Directors or advisory committees, Research Funding; Takeda: Membership on an entity's Board of Directors or advisory committees, Research Funding. Bustoros: Menarini: Consultancy; Janssen: Consultancy; Takeda: Consultancy; Epizyme: Consultancy; BMS: Consultancy; Karyopharm: Consultancy. Bianchi: Prothena: Consultancy. O'Donnell: BMS: Honoraria; Sanofi: Honoraria; Pfizer: Honoraria; Janssen: Honoraria; Takeda: Consultancy; Natera: Other: Steering committee; Exact Sciences: Consultancy. Anderson: Janssen: Consultancy; Window: Membership on an entity's Board of Directors or advisory committees; Genentech: Consultancy; Dynamic Cell Therapies: Membership on an entity's Board of Directors or advisory committees; C4 Therapeutics: Membership on an entity's Board of Directors or advisory committees; Pfizer: Consultancy; Amgen: Consultancy; AstraZeneca: Consultancy; Starton Therapeutics: Membership on an entity's Board of Directors or advisory committees. Getz: Scorpion Therapeutics: Consultancy, Current equity holder in private company, Other: Founder; IBM, Pharmacyclics/Abbvie, Bayer, Genentech, Calico, and Ultima Genomics: Research Funding; Broad Institute: Patents & Royalties: MSMuTect, MSMutSig, POLYSOLVER, SignatureAnalyzer-GPU, MSEye, and MinimuMM-seq; PreDICTA Biosciences: Consultancy, Current equity holder in private company, Other: Founder. Richardson: Celgene/Bristol Myers Squibb, GSK, Karyopharm Therapeutics, Oncopeptides, Regeneron, Sanofi: Consultancy; Oncopeptides: Research Funding. Sklavenitis-Pistofidis: PreDICTA Biosciences: Consultancy, Current equity holder in private company, Other: Co-founder. Ghobrial: Pfizer: Consultancy, Other: Speaker fees; Sanofi: Consultancy; Takeda: Consultancy, Other: Speaker fees; Regeneron: Consultancy, Other: Speaker fees; Binding Site, part of Thermo Fisher Scientific: Consultancy; GlaxoSmithKline: Consultancy; Oncopeptides: Consultancy; Novartis: Consultancy; Bristol Myers Squibb: Consultancy, Other: Speaker fees; Menarini Silicon Biosystems: Consultancy, Other: Speaker fees; Huron Consulting: Consultancy; Janssen: Consultancy, Other: Speaker fees; 10X Genomics: Consultancy; CurioScience: Consultancy, Other: Speaker fees; Window Therapeutics: Consultancy; Vor Biopharma: Other: Speaker fees; Standard Biotools: Other: Speaker fees; Aptitude Health: Consultancy; Adaptive: Consultancy; AbbVie: Consultancy; Amgen: Consultancy, Other: Speaker fees; PreDICTA Bioscience: Consultancy, Current equity holder in private company, Membership on an entity's Board of Directors or advisory committees, Other: Co-founder; Disc Medicine: Current equity holder in private company, Membership on an entity's Board of Directors or advisory committees.

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