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1150 Transcriptomic Analysis of the Functions of CSF1R-Dependent Macrophages in Postnatal Development in the Rat

Program: Oral and Poster Abstracts
Session: 201. Granulocytes, Monocytes, and Macrophages: Poster I
Hematology Disease Topics & Pathways:
Research, Fundamental Science, Hematopoiesis, Immunology, Biological Processes
Saturday, December 7, 2024, 5:30 PM-7:30 PM

Dylan Carter-Cusack, BSc (Honours)1*, Stephen Huang, PhD2*, Sahar Keshvari, PhD3*, Omkar Patkar, PhD3*, Anuj Sehgal, PhD3*, Rachel Allavena4*, Robert Byrne, PhD5*, Paul Morgan, PhD5*, Stephen Bush, PhD6*, Kim Z Summers, PhD7*, Katharine M Irvine, BSc (Hons), LLB, PhD7* and David A Hume, BScHons, PhD7*

1Translational Research Institute, Brisbane, Mater Research Institute-University of Queensland, Brisbane, QLD, Australia
2Translational Research Institute, Mater Research Institute - UQ, Brisbane, Australia
3Translational Research Institute, Mater Research Institute - University of Queensland, Brisbane, Australia
4University of Queensland, School of Veterinary Sciences, The University of Queensland, Brisbane, Australia
5Cardiff University, UK Dementia Research Institute Cardiff, School of Medicine Cardiff University, Cardiff, United Kingdom
6Xi'an Jiaotong University, School of Automation Science, Xi'an Jiaotong University, Xian, China
7Translational Research Institute, Mater Research Institute-University of Queensland, Brisbane, QLD, Australia

Adaptation to existence outside the womb is a key event in the life of a mammal. The absence of macrophages in rats with a homozygous mutation in the Csf1r gene (Csf1rko) severely compromises pre-weaning somatic growth and maturation of organ function. Transfer of wild-type bone marrow cells (BMT) at weaning rescues tissue macrophage populations permitting normal development and long-term survival. To dissect the phenotype and function of macrophages in postnatal development, we generated transcriptomic profiles of all major organs of wild-type and Csf1rko rats at weaning and selected organs following rescue by BMT. The transcriptomic profiles revealed subtle effects of macrophage deficiency on development of all major organs. Network analysis revealed a common signature of CSF1R-dependent resident tissue macrophages that includes the components of complement C1Q (C1qa/b/c genes). Circulating C1Q was almost undetectable in Csf1rko rats and rapidly restored to normal levels following BMT. Tissue-specific macrophage signatures were also identified, notably including sinus macrophage populations in the lymph nodes that have not previously been identified. Their loss in Csf1rko rats was confirmed by immunohistochemical localisation of CD209B (SIGNR1). By 6-12 weeks, Csf1rko rats succumb to emphysema-like pathology associated with the selective loss of interstitial macrophages and granulocytosis. This pathology was prevented by BMT. Along with physiological rescue, BMT precisely regenerated the abundance and expression profiles of resident macrophages. The exception was the brain, where BM-derived microglia-like cells had a distinct expression profile compared to resident microglia. In addition, the transferred BM failed to restore blood monocyte or CSF1R-positive bone marrow progenitors. Considering the integrated data we provide insight into the inter-related systemic consequences of developmental delay in bone, liver and pituitary and potential contributions to somatic growth deficiency in Csf1rko rats.

Disclosures: Summers: Sanius Health: Current Employment.

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