Session: 602. Myeloid Oncogenesis: Basic: Poster II
Hematology Disease Topics & Pathways:
Research, Fundamental Science, Acute Myeloid Malignancies, AML, Translational Research, Diseases, Myeloid Malignancies, Biological Processes, Molecular biology, Pathogenesis
Of the nucleocytoplasmic genes, CSE1L was the top differentially expressed exportin in young vs older AML patients. CSE1L is an exportin with a role in recycling importin alpha proteins. Recently, CSE1L was found to export circular RNA. Its function in childhood/young adult AML is unknown. By immunoblotting, we confirmed increased expression of CSE1L protein in childhood/young (n=10, mean age 23) vs older (n=10, mean age 77) AML. CSE1L mRNA expression predicted outcome exclusively in young patients (<40 years), where increased CSE1L predicted for decreased remission rates (P=0.027), event free survival (P <0.0001) and overall survival (P<0.0001), but was not predictive in older patients (TARGET, Beat AML) .
To further understand the dependency of childhood/young adult AML on CSE1L, we knocked down the gene with shRNA in AML cell lines derived from young patients (NB4, MV4-11, THP-1) and TEX cells, derived from transducing cord blood with a high-risk pediatric fusion, FUS-ERG. CSE1L knockdown reduced AML growth, viability and clonogenicity. We confirmed loss of AML viability and clonogenicity using CSE1L CRISPR knockout. Gene dependency scores indicated a greater reliance on CSE1L than XPO1 in young AML cell lines (P<0.0001). Knockdown of CSE1L in primary AML cells from a young chemo-refractory patient with high CSE1L expression, reduced leukemic engraftment into mouse marrow. CSE1L depletion also reduced AML engraftment in secondary transplants, demonstrating its essentially for leukemic stem cells. In contrast, knockdown of CSE1L did not impair engraftment of normal human hematopoietic cells into mouse marrow.
To understand the function of CSE1L, we interrogated a BioID database (PXD007976) and identified proteins that interact with CSE1L. Among the CSE1L interactors, ribosome biogenesis was the top biologic process identified. We validated the interaction with ribosome biogenesis proteins and BioID hits, RRS1 and RPL29 using Co-IP and proximity ligation assay. Knockdown of CSE1L in TEX cells led to nuclear accumulation of RPL29 and a reduction in cytoplasmic RPL29 (3.3 fold). Using a GFP reporter assay that incorporates into the large ribosomal subunit precursor (pre-60S), we induced depletion of CSE1L and confirmed decreased cytoplasmic RPL29-GFP. Pre-ribosome export is closely coordinated with rRNA maturation and pre-ribosome assembly. CSE1L knockdown decreased the expression of pre-60S and ribosome biogenesis pathways and quantitively reduced rRNA transcription rates by PCR (3 fold), consistent with a role for CSE1L in large ribosome subunit export and ribosome biogenesis.
Given increased CSE1L expression in young patients and its role in ribosome biogenesis, next we investigated ribosome biogenesis in AML patients. Ribosome biogenesis pathways were the top differentially expressed gene sets in young vs older AML patients (BeatAML P=1e-10, FDR=7.9e-8, TCGA P=2.34e-4, FDR=3.4e-3). Expression of ribosome biogenesis pathways positively correlated with expression of the nucleocytoplasmic transport pathway.
In summary, nucleocytoplasmic transport and ribosome biogenesis are upregulated in young AML patients. The nuclear exportin, CSE1L, is a regulator of ribosome biogenesis and its depletion leads to impaired ribosome biogenesis and leukemic cell death in childhood/young adult AML. Thus, our findings reinforce growing evidence that childhood and older adult AML are fundamentally different diseases, driven by different biologic processes.
Disclosures: Dick: Bristol-Myers Squibb/Celgene: Research Funding; Pfizer/Trillium Therapeutics: Patents & Royalties: IP interest in SIRP-a therapeutics. Schimmer: Takeda: Consultancy, Research Funding; BMS: Research Funding; Medivir AB: Research Funding; Novartis: Consultancy; Jazz: Consultancy; Otsuka Pharmaceuticals: Consultancy; UHN: Patents & Royalties: DNT cells.