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2810 Allogeneic Hematopoietic Stem Cell Transplantation Following CAR T Therapy in Relapsed/Refractory Acute Lymphoblastic Leukemia

Program: Oral and Poster Abstracts
Session: 613. Acute Lymphoblastic Leukemias: Therapies Excluding Allogeneic Transplantation: Poster II
Hematology Disease Topics & Pathways:
Research, Lymphoid Leukemias, ALL, Adult, Clinical Research, Pediatric, Diseases, Therapy sequence, Real-world evidence, Treatment Considerations, Lymphoid Malignancies, Young adult , Study Population, Human, Measurable Residual Disease
Sunday, December 8, 2024, 6:00 PM-8:00 PM

Natalie Rafaeli, MD1, David Marin, MD2, Celina Ledesma2*, Nitin Jain, MD3, Priti Tewari, MD4*, Issa F. Khouri, MD2, Amanda L. Olson, MD5, Gheath Alatrash, PhD, DO6, Nicholas J. Short, MD3, Elias Jabbour, MD5, Katy Rezvani, MD7, Amin M. Alousi, MD2*, Uday Popat, MD2, Richard E. Champlin, MD2, Elizabeth J. Shpall, MD2 and Partow Kebriaei, MD1

1MD Anderson Cancer Center, Houston, TX
2Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas MD Anderson Cancer Center, Houston, TX
3Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX
4Department of Pediatrics, Pediatric Stem Cell Transplantation and Cellular Therapy, University of Texas MD Anderson Cancer Center, Houston, TX
5The University of Texas MD Anderson Cancer Center, Houston, TX
6Department of Stem Cell Transplantation and Cellular Therapy, MD Anderson Cancer Center, Pearland, TX
7Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas M D Anderson Cancer Center, Houston, TX

In patients with relapsed/refractory (R/R) acute lymphoblastic leukemia (ALL), CAR-T cells have shown very high complete remission (CR) rates with two therapies approved in this setting, tisagenlecleucel and brexucabtagene autoleucel with overall response rates of 81% and 71%, respectively (1,2). Despite being a highly efficacious therapy, relapse is still an issue among a subset of patients (3). Park, et al. evaluated 17 among 53 patients who received consolidative HSCT with no significant difference in event free survival (EFS) or OS compared to the non-transplanted group (3). Other retrospective studies have evaluated the effect of consolidative HSCT following CAR T therapy; however, most of these studies have smaller patient sizes and discordant findings (1,4–10). Due to conflicting findings, the role of transplant in this setting remains unclear.

A total of 30 patients with R/R ALL with prior CAR T therapy followed by HSCT at MDACC from January 2014 through December 2023 were identified. Patients who had received prior HSCT or previous CAR-T therapies were included. HSCT consolidation was defined as no relapse or intervening therapy between CAR-T and transplant therapy.

The median age was 24 years, nine pts (30%) were BCR::ABL1-like (Ph like) and 13 (43.4%) pts had additional high-risk features. MRD by multiparameter flow cytometry, or PCR in Ph positive patients, was detected in 9 patients (30%) prior to HSCT. The patients were a heavily pretreated population with 9 (30%) patients with a prior HSCT and the median number of prior lines of therapy being 4; 17 patients (57%) were in CR 3 or more advanced remission at time of HSCT. Fifteen patients (50%) received HSCT as consolidation following CAR-T therapy. The median time between CAR-T therapy to HSCT was 5.5 months for all patients, and 3.4 months in the consolidative transplant group.

The 12-month OS, PFS and TRM rates for the entire cohort was 51.1%, 35.0% and 29.3%, respectively. Using multivariate analysis, a significant difference in OS was found for patients who were in CR ≤2 as opposed to ≥3 (HR-4.787, CI 1.159-19.775, p=0.031) at time of transplant, in patients who had received fewer lines of chemotherapy (≤3 vs. ≥4) (HR-7.021, CI 1.720-28.659, p=0.007) and in the older patient population (age ≥25 vs. <25) (HR-0.123, CI-0.028-0.538, p=0.005). Using univariate analysis, patients who had received ≥4 lines of chemotherapy had significantly worse OS and PFS (12-month OS 70.1 vs. 35.2, p=0.02; 12-month PFS 48.2 vs. 23.4, p=0.041). There was a difference in 12-month OS, PFS and TRM rates in patients in CR≤2 compared to ≥3 (741. Vs. 33.6, p=0.024; 59.3 vs. 17.6, p=0.011, 100 vs. 52.5, p=0.004). There was no difference found in OS in patients who were MRD positive, although there was a worse 12-month PFS and 12-month relapse (42.5 vs. 0, p=0.024; 37.6 vs. 100, p=0.003). HSCT as use of consolidation showed a trend in 12-month OS (33.3 vs. 72 mos., p=0.055). A shorter duration between CAR-T to HSCT compared by <4 months or ≥4 months showed a trend towards significance in 12-month OS (77.1 vs. 40 mos., p=0.071).

There was a significant increase in OS in patients who were in an earlier remission status (CR≤2), patients who had received fewer lines of treatment (≤3) and patients who were older (ages >25). Increased TRM is likely due to being a very heavily treated pediatric population. The use of HSCT as consolidation had a trend towards significance in OS as did shorter duration (<4 months) between the CAR T and HSCT. Although the PFS and relapse rates were improved with MRD negativity prior to HSCT, likely due to the small patient cohort, no significant improvement in OS was found. For the patients who received HSCT as consolidation vs. those who did not, the TRM was 21% vs. 39.8%, respectively, p=0.355. The relapse rate was 44.1% vs. 53.6%, respectively, p=0.517.

Our data shows that CAR T therapy with HSCT used earlier in the patient’s treatment course is associated with improved survival, with trend for improved survival in patients who received consolidative HSCT following CAR T and a trend towards decreased TRM and lower relapse rates with consolidative HSCT. Standard approach regarding sequencing of therapies still remains with a critical knowledge gap. Further larger trials are needed to determine if consolidation with transplant can ultimately cure patients who remain MRD positive after CAR T therapy, and to identify patient subsets who may benefit from HSCT following CAR T therapy.

Disclosures: Jain: TG Therapeutics: Consultancy, Honoraria, Other: Travel Support; Bristol Myers Squibb: Consultancy, Honoraria, Other: Travel Support, Research Funding; Dialectic Therapeutics: Research Funding; Cellectis: Consultancy, Honoraria, Other: Travel Support, Research Funding; CareDx: Consultancy, Honoraria, Other: Travel Support; BeiGene: Consultancy, Honoraria, Other: Travel Support; Medisix: Research Funding; MingSight: Honoraria, Research Funding; MEI Pharma: Consultancy, Honoraria, Other: Travel Support; Kite, a Gilead Company: Consultancy, Honoraria, Other: Travel Support, Research Funding; TransThera Sciences: Research Funding; Precision Biosciences: Consultancy, Honoraria, Other: Travel Support, Research Funding; Pfizer: Research Funding; ADC Therapeutics: Research Funding; Takeda: Research Funding; Incyte: Research Funding; Genentech: Consultancy, Honoraria, Other: Travel Support, Research Funding; Newave: Research Funding; Fate Therapeutics: Research Funding; Aprea Therapeutics: Research Funding; Loxo Oncology: Research Funding; AstraZeneca: Consultancy, Honoraria, Other: Travel Support, Research Funding; NovalGen: Research Funding; Ipsen: Consultancy, Honoraria, Other: Travel Support; Janssen: Consultancy, Honoraria, Other: Travel Support; Servier: Research Funding; Pharmacyclics: Consultancy, Honoraria, Other: Travel Support, Research Funding; Adaptive Biotechnologies: Consultancy, Honoraria, Other: Travel Support, Research Funding; AbbVie: Consultancy, Honoraria, Other: Travel Support, Research Funding. Khouri: Pfizer: Research Funding; Bristol Myers Squibb: Research Funding. Short: Takeda Oncology: Honoraria, Research Funding; NextCure: Research Funding; Sanofi: Honoraria; Autolus: Honoraria; BeiGene: Honoraria; Novartis: Honoraria; GSK: Consultancy, Research Funding; Amgen: Honoraria; Xencor: Research Funding; Adaptive Biotechnologies: Honoraria; Pfizer Inc.: Honoraria; Astellas Pharma, Inc.: Honoraria, Research Funding; Stemline Therapeutics: Research Funding. Jabbour: AbbVie, Adaptive Biotechnologies, Amgen, Astellas Pharma, BMS, Genentech, Incyte, Pfizer, Takeda: Consultancy; AbbVie, Adaptive Biotechnologies, Amgen, Ascentage Pharma Group, Pfizer, Takeda: Research Funding. Rezvani: Takeda: Other: License agreement and Research Agreement, Patents & Royalties; Affimed: Other: License agreement and Research Agreement, Patents & Royalties; Avenge Bio: Membership on an entity's Board of Directors or advisory committees; Navan Technologies Inc: Membership on an entity's Board of Directors or advisory committees; Caribou: Membership on an entity's Board of Directors or advisory committees; Virogin Biotech Canada Limited: Membership on an entity's Board of Directors or advisory committees; Bit Bio Limited: Membership on an entity's Board of Directors or advisory committees; Replay Holdings: Membership on an entity's Board of Directors or advisory committees; oNKo Innate: Membership on an entity's Board of Directors or advisory committees; The Alliance for Cancer Gene Therapy ACGT: Membership on an entity's Board of Directors or advisory committees; Innate Pharma: Membership on an entity's Board of Directors or advisory committees. Popat: Incyte: Research Funding; Bayer: Research Funding; T Scan: Research Funding; Abbvie: Research Funding. Shpall: Zelluna Immunotherapy: Other: Scientific Advisor; Axio Research: Current Employment, Other: Scientific Advisor; FibroBiologics: Other: Scientific Advisor; Adaptimmune Limited: Other: Scientific Advisor; National Marrow Donor Program: Other: Board of Directors/Management. Kebriaei: Jazz Pharmaceuticals: Consultancy, Honoraria; Pfizer: Consultancy, Honoraria.

*signifies non-member of ASH