65th ASH Annual Meeting & Exposition: 651. Multiple Myeloma and Plasma Cell Dyscrasias: Basic and Translational: Spatial Dissection and Multiomics Analysis of the Multiple Myeloma Tumor and Immune Microenvironment

Symposia: Multiple Myeloma and Plasma Cell Dyscrasias: Basic and Translational Oral and Poster Abstracts
Oral

genomics, bioinformatics, immune mechanism, immunology, computational biology, Biological Processes, Technology and Procedures

Harbor Ballroom (Manchester Grand Hyatt San Diego)

Moderators:

Jerome Moreaux, PhD, Institute of Human Genetics, UMR 9002 CNRS-UM and David Coffey, MD, University of Miami

Disclosures:

No relevant conflicts of interest to declare.

This session addresses the comprehensive characterization of the immune microenvironment within the bone marrow and peripheral blood of patients with multiple myeloma. Utilizing cutting-edge research methodologies, including spatial dissection, single-cell RNA-Seq, ATAC-Seq, and multi-omics analysis, the investigators report on the intricate interactions within the tumor microenvironment, the evolution of the disease, and the role of immune effector cells.

9:30 AM

Spatial Dissection of the Bone Marrow Microenvironment in Multiple Myeloma By High Dimensional Multiplex Tissue Imaging

Marc-Andrea Baertsch, MD^1,2,3^*, Alexander Brobeil, MD⁴^*, John Hickey, PhD³^*, Maximilian Haist, MD³^*, Alexandra Maria Poos, PhD⁵^*, Guolan Lu, PhD³^*, Wilson Kuswanto, MD, PhD³^*, Christian Schuerch, MD, PhD⁶^*, Harald Voehringer, PhD⁷^*, Wolfgang Huber⁸^*, Gunhild Mechtersheimer, MD⁹^*, Carsten Mueller-Tidow, MD¹⁰^*, Peter Schirmacher, MD⁴^*, Katja Weisel, MD¹¹, Roland Fenk, MD, PhD¹²^*, Hartmut Goldschmidt^13,14, Yury Goltsev, PhD¹⁵^*, Marc S. Raab^10,14,16^*, Niels Weinhold, PhD¹⁰^* and Garry P. Nolan, PhD¹⁷^*

¹Department of Hematology, Oncology and Rheumatology, University Hospital Heidelberg, Heidelberg, Germany
²Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), Heidelberg, Germany
³Department of Microbiology and Immunology, Stanford University, Stanford, CA
⁴Department of Pathology, Heidelberg University Hospital, Heidelberg, Germany
⁵Heidelberg Myeloma Center, Department of Internal Medicine V, University Hospital Heidelberg, Heidelberg, Germany
⁶Department of Pathology and Neuropathology, Tuebingen University Hospital, Tuebingen, Germany
⁷European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
⁸Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
⁹Institute of Pathology, Heidelberg, Germany
¹⁰Department of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, Heidelberg, Germany
¹¹Department of Oncology, Hematology and Bone Marrow Transplantation with Section of Pneumology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, Hamburg, Germany
¹²Department of Hematology, Oncology and Clinical Immunology,, University Hospital Düsseldorf, Duesseldorf, Germany
¹³Internal Medicine V, GMMG-Study Group, University Hospital Heidelberg, Heidelberg, Germany
¹⁴National Center for Tumor Diseases (NCT), Heidelberg University Hospital, Heidelberg, Germany
¹⁵Stanford Medical School, Stanford, CA
¹⁶Clinical Cooperation Unit (CCU) Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), Heidelberg, Germany
¹⁷Department of Pathology, Stanford University, Stanford, CA

9:45 AM

Integrative Single-Cell RNA-Seq and Single-Cell ATAC-Seq Analysis of Human Plasma Cell Differentiation

Elina Alaterre, PhD^1,2^*, Sara Ovejero, PhD³^*, Marion Espéli, PhD^4,5^*, Thierry Fest, MD, PhD^6,7^*, Michel Cogné, MD, PhD^6,8^*, Pierre Milpied, PhD⁹^*, Giacomo Cavalli, PhD²^* and Jerome Moreaux, PhD^10,11,12

¹Diag2tec, Montpellier, France
²Institute of Human Genetics, CNRS, Montpellier, France
³Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology, Montpellier University Hospital Center, Montpellier, France
⁴Institut de Recherche Saint-Louis, INSERM UMR 1160, Paris, France
⁵OPALE Carnot Institute, Hôpital St-Louis, Paris, France
⁶Inserm, Rennes University, Rennes, France
⁷INSERM, Établissement Français du Sang de Bretagne, Team B_DEVIL, UMR_S1236, Université de Rennes 1, Rennes, France
⁸Centre National de la Recherche Scientifique (CNRS), Limoges University, Limoges, France
⁹Aix Marseille Université, CNRS, INSERM, Centre d'Immunologie de Marseille-Luminy, Marseille, France
¹⁰Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology, Montpellier University Hospital, Montpellier, France
¹¹Université Montpellier, Montpellier, France
¹²Institute of Human Genetics, CNRS UM UMR 9002, University of Montpellier, MONTPELLIER, France

10:00 AM

Single Nuclei Multiomics Analysis of Transcriptional and Chromatin Accessibility of Tumor Cells Uncovers Molecular Signatures and Regulatory Elements of Malignant Clonal Evolution in Multiple Myeloma

Visanu Wanchai, PhD¹^*, Hongwei Xu, DDS²^*, Cody Ashby, PhD³^*, Frits van Rhee, MD, PhD³, Guido Tricot, MD¹^*, John D Shaughnessy, Jr, PhD⁴^* and Fenghuang Zhan, MD, PhD³

¹Myeloma Center, Winthrop P. Rockefeller Cancer Institute, University of Arkansas For Medical Sciences, Little Rock, AR
²Myeloma Center, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical, Little Rock, AR
³Myeloma Center, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR
⁴University of Arkansas For Medical Sciences, Little Rock, AR

10:15 AM

Comprehensive Characterization of the Bone Marrow Microenvironment Transcriptional Remodeling in the Progression from MGUS to Smoldering and Multiple Myeloma

Itziar Cenzano, PhD¹^*, Miguel Cocera¹^*, Azari Bantan²^*, Marta Larrayoz, PhD³^*, Amaia Vilas-Zornoza¹^*, Patxi San-Martin¹^*, Paula Aguirre-Ruiz³^*, Beñat Ariceta¹^*, Jin Ye²^*, Diego Alignani¹^*, Aitziber Lopez¹^*, Aleksandra Kurowska²^*, Fatimah AlSultan²^*, Raghad Shuwaikan¹^*, Vincenzo Lagani, PhD²^*, Jesper Tegner, PhD²^*, Iñaki I. Martin Subero, PhD^4,5^*, Jose A. A Martinez-Climent, MD¹, Xabier Agirre, PhD¹^*, Borja Saez-Ochoa, PhD³^*, Bruno Paiva¹^*, Jesus San-Miguel, MD, PhD^6,7, Mikel Hernaez, PhD⁸^*, Isabel A Calvo, PhD³^*, David Gomez-Cabrero, PhD^2,9^* and Felipe Prosper, MD, PhD^10,11,12

¹Hematology and Oncology Program, Centre for Applied Medical Research (CIMA), Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Cancer Center Clinica Universidad de Navarra (CCUN), Pamplona, Spain
²Bioscience Program, Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology KAUST, Thuwal, Saudi Arabia
³Hematology and Oncology Program, Centre for Applied Medical Research (CIMA), Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Cancer Center Clinica Universidad de Navarra (CCUN), Pamplona, Spain, Pamplona, Spain
⁴Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain
⁵Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
⁶Hematology and Cell Therapy Department. Clinica Universidad de Navarra, Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Cancer Center Clinica Universidad de Navarra (CCUN), Pamplona, Spain
⁷Centro de Investigacion Biomedica en Red de Cancer (CIBERONC), Madrid, Spain
⁸Computational Biology Program. Cima Universidad de Navarra. IdiSNA., Pamplona, Spain
⁹Translational Bioinformatics Unit, Navarrabiomed, Universidad Pública de Navarra (UPNA), Pamplona, Spain
¹⁰Cancer Center Clinica Universidad de Navarra, Centro de Investigación Médica Aplicada (CIMA), IDISNA, CIBER-ONC number CB16/12/00369 and CB16/12/00489, Pamplona, Spain
¹¹Centro de Investigacion Biomedica en Red de Cancer (CIBERONC)., Madrid, Spain
¹²Hematology and Cell Therapy Department. Clinica Universidad de Navarra, IdiSNA., Pamplona, Spain

10:30 AM

Single Cell Multi-Omic Analysis of Tumor Microenvironment Evolution across the Disease Spectrum of Multiple Myeloma Identifies Differential Mechanisms of Immune Suppression

Minghao Dang, PhD¹^*, Hima Bansal, PhD²^*, Maria Jose Acevedo-Calado, PhD²^*, Li Qin, PhD²^*, Wei Tan, PhD²^*, Luz Yurany Moreno Moreno Rueda, PhD, MSc²^*, Mei Huang, MS²^*, David Berrios Nolasco²^*, Hans Lee, MD³, Krina K. Patel, MD, MSc², Pei Lin, MD, DM, MDPC⁴, Sheeba K. Thomas, MD², Donna M. Weber, MD², Linghua Wang, MD, PhD¹^*, Elisabet E. Manasanch, MD³ and Robert Z. Orlowski, MD, PhD^2,5

¹Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX
²Department of Lymphoma & Myeloma, The University of Texas MD Anderson Cancer Center, Houston, TX
³Department of Lymphoma/Myeloma, The University of Texas MD Anderson Cancer Center, Houston, TX
⁴Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX
⁵Department of Experimental Therapeutics, MD Anderson Cancer Center, Houston, TX

10:45 AM

The Peripheral Blood Stem Cell Immunome Demonstrates Abnormal Immune Effector Cells Early in the Disease Course of Multiple Myeloma and Contributes to Inferior Outcomes and Secondary Myeloid Neoplasms

Saurabh Zanwar, M.B.B.S., M.D.¹, Eapen K. Jacob, MD², Carl Greiner³^*, Kevin Pavelko, Ph.D.⁴^*, Michael Strausbauch⁴^*, Emilie Anderson, M.S.⁵^*, Arini Arsana⁶^*, Megan Weivoda, PhD⁵, Mithun V Shah, MD, PhD⁵ and Taxiarchis Kourelis, MD⁵

¹Division of Hematology, Department of Internal Medicine, Mayo Clinic, Rochester, MN
²Mayo Clinic College of Medicine, Rochester, MN
³Mayo Clinic, Rocheser, MN
⁴Mayo Clinic, Rochester, MN
⁵Division of Hematology, Mayo Clinic, Rochester, MN
⁶Mayo Clinic, Rochester

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651A.O1.6 651. Multiple Myeloma and Plasma Cell Dyscrasias: Basic and Translational: Spatial Dissection and Multiomics Analysis of the Multiple Myeloma Tumor and Immune Microenvironment