Session: 503. Clonal Hematopoiesis, Aging and Inflammation: Poster I
Hematology Disease Topics & Pathways:
Acute Myeloid Malignancies, AML, Research, adult, CHIP, Translational Research, Diseases, Therapies, Biological Processes, Myeloid Malignancies, Human, Study Population
Here, we performed an in-depth analysis of CH in whole-blood (WB) and plasma of patients with relapsed high-grade ovarian cancer (HGOC) receiving platinum-based chemotherapy and PARPi treatment, elucidating the mutational landscape of CH, gene-specific dynamics, as well as the potential interference of CH with putative tumor-derived mutations in cell-free DNA (cfDNA). Serial specimen of 103 patients with relapsed, platinum-sensitive HGOC participating in the European Trial on Enhanced DNA Repair Inhibition in Ovarian Cancer (EUDARIO) study (ENGOT-ov48/NCT03783949) were analyzed using error-corrected targeted sequencing with a custom panel covering 45 myeloid and 27 HR-related genes. Study treatment consisted of six cycles of carboplatin-based chemotherapy followed by maintenance therapy with Niraparib ± the HSP90 inhibitor Ganetespib.
A total of 128 somatic mutations with variant allele frequencies (VAFs) ≥ 1% were identified in 58 patients (56%) with a high fraction of DDR mutations (51 PPM1D mutations in 27 patients, 7 TP53 mutations in 7 patients). Age (median 62 years) and the number of previous therapy lines (median 1, range 1-5 lines) were significantly associated with the presence of CH. Specifically, prior exposure to PARPi treatment was strongly associated with both the presence of CH and the number of CH mutations. CH-positive patients had significantly shorter progression-free survival (median 7.9 months vs 10.6 months, p = 0.021 in log-rank test), and a trend for shorter overall survival (median 21.1 months vs 27.7 months, p = 0.16) in univariate survival analysis. With respect to adverse events, CH-positive patients more frequently had infections during study treatment (49% vs 26%, p = 0.025).
All but 5 CH mutations with VAF ≥ 1% were also detectable in cfDNA with significant correlation of VAFs (R = 0.84, p < 0.0001). Mutations occurring solely in cfDNA (putatively tumor-derived) had a high fraction of TP53 and HR-related mutations, while DNMT3A and PPM1D, were rarely mutated (Figure 1). However, 23/87 (26%) of TP53 mutations and 31/98 (32%) of mutations in HR-related genes detected in cfDNA were of hematopoietic origin, underlining the importance of parallel sequencing of WB in liquid biopsies to avoid false positive results.
The dynamics of CH clones under carboplatin and PARPi treatment were assessed in paired WB samples at initiation/end of treatment, which were available for 62 patients. 96 clones that initially had a VAF < 1% emerged during the treatment, including 56 PPM1D- and 10 TP53-mutated clones. Gene-wise comparison revealed a significantly higher median clonal fitness in PPM1D- and TP53-mutated clones compared to TET2- or DNMT3A-mutated clones (Figure 2). PPM1D-mutated clones exhibited lower fitness during PARPi treatment compared to carboplatin treatment (p = 0.0015 in Wilcoxon rank sum test), which was not the case for TP53-mutated clones.
Finally, 8 samples with multiple mutations were subjected to single-cell genotyping on the Mission Bio Tapestri platform to investigate their clonal architecture. Preliminary data indicate clonal exclusiveness of co-occurring DDR mutations.
In summary, our data reveals a high prevalence of CH in patients with relapsed HGOC and provides novel insights into the clonal architecture and dynamics of CH under carboplatin and PARPi treatment with a differential selection of DDR-driven clones. Moreover, we report a relevant interference of CH-derived mutations with tumor-derived mutations in cfDNA.
Disclosures: Concin: ImmunoGen: Consultancy; Seagen: Consultancy; Akesobio: Consultancy; Ensai: Consultancy; GSK: Consultancy; Astra Zeneca: Consultancy; Mersana: Consultancy; Seattle Genetics: Consultancy; Kartos: Consultancy, Other: Educational fees; eTheRNA immunotherapies NV: Consultancy; Roche: Other: Travel expenses; Genmab: Other: Travel expenses; Amgen: Other: Travel expenses; MSD: Other: Educational fees; Medscape Oncology: Other: Educational fees; TouchIME: Other: Educational fees. Sehouli: Vifor Pharma: Honoraria; Hexal AG: Honoraria; Eisai: Consultancy; Novocure: Consultancy; Oncoinvent: Consultancy; Novartis: Honoraria; MSD: Consultancy; Tesaro: Consultancy, Honoraria, Research Funding; PharmaMar: Consultancy, Honoraria; GlaxoSmith: Consultancy, Honoraria, Research Funding; Lilly: Research Funding; Bayer: Honoraria, Research Funding; Clovis: Consultancy, Honoraria, Research Funding; Roche: Consultancy, Honoraria, Research Funding; AstraZeneca: Consultancy, Honoraria, Research Funding. Ray-Coquard: MSD: Consultancy, Research Funding; Roche: Consultancy, Honoraria, Research Funding; BMS: Research Funding; Novartis: Consultancy, Research Funding; Astra Zeneca: Consultancy, Honoraria, Research Funding; Merck Sereno: Consultancy, Research Funding; GSK: Consultancy, Honoraria; AbbVie: Consultancy; Agenus: Consultancy; Advaxis: Consultancy; ESAI: Consultancy; Daichi: Consultancy; PharmaMar: Consultancy; Genmab: Consultancy; Pfizer: Consultancy; Deciphera: Consultancy; Mersana: Consultancy; Amgen: Consultancy; Tesaro: Consultancy; Clovis: Consultancy. Zamagni: MSD: Membership on an entity's Board of Directors or advisory committees, Research Funding; Celgene: Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Eisai: Membership on an entity's Board of Directors or advisory committees, Research Funding; Roche: Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Novartis: Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Astra Zeneca: Membership on an entity's Board of Directors or advisory committees, Research Funding; Daiichi Sankyo: Membership on an entity's Board of Directors or advisory committees, Research Funding; Amgen: Membership on an entity's Board of Directors or advisory committees; GSK: Membership on an entity's Board of Directors or advisory committees, Research Funding; PharmaMar: Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Lilly: Membership on an entity's Board of Directors or advisory committees, Research Funding; Pfizer: Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Tesaro: Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Pierre Fabre: Other: Travel expenses, Research Funding; Istituto Gentili: Other: Travel expenses, Research Funding; Teva: Research Funding; Seagen: Research Funding; QuintilesIMS: Membership on an entity's Board of Directors or advisory committees. Vergote: Agenus: Consultancy; Akesobio: Consultancy; Astra Zeneca: Consultancy; Bristol Myers Squibb: Consultancy; Deciphera Pharmaceuticals: Consultancy; Karyopharm: Consultancy, Other: Travel expenses; Mersana: Consultancy; MSD: Consultancy; Novocure: Consultancy, Other: Travel expenses; Eisai: Consultancy; Elevar Therapeutics: Consultancy; F. Hoffmann-La Roche: Consultancy; Genmab: Consultancy, Other: Travel expenses; GSK: Consultancy; Immunogen: Consultancy; Jazzpharma: Consultancy; Novartis: Consultancy; Oncoinvent: Consultancy, Other: Contracted research; OncYerna: Consultancy; Sanofi: Consultancy; Regeneron: Consultancy; Seagen: Consultancy; Sotio: Consultancy; Verastem Oncology: Consultancy; Zentalis: Consultancy; Amgen: Research Funding; Roche: Research Funding. Lorusso: Sutro: Membership on an entity's Board of Directors or advisory committees; Novartis: Consultancy, Research Funding; Genmab: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Immunogen: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; MSD: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Roche: Research Funding; Seagen: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Pharmamar: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Oncoinvest: Membership on an entity's Board of Directors or advisory committees; GSK: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Corcept: Membership on an entity's Board of Directors or advisory committees; Clovis Oncology: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding; Astra Zeneca: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: Travel expenses, Research Funding. Bullinger: Bristol-Myers Squibb: Honoraria; Amgen: Honoraria; Janssen: Honoraria, Membership on an entity's Board of Directors or advisory committees; Pfizer: Honoraria, Membership on an entity's Board of Directors or advisory committees; Bayer Oncology: Research Funding; Jazz Pharmaceuticals: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; Abbvie: Honoraria, Membership on an entity's Board of Directors or advisory committees; Gilead: Honoraria, Membership on an entity's Board of Directors or advisory committees; Astellas: Honoraria; Celgene/BMS: Honoraria, Membership on an entity's Board of Directors or advisory committees; Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees; Daiichi Sankyo: Honoraria; Sanofi: Honoraria. Braicu: Bayer: Research Funding; Merck: Honoraria, Research Funding; GSK: Consultancy, Honoraria, Research Funding; Astra Zeneca: Consultancy, Honoraria, Other: Travel expenses, Research Funding; Resolve: Research Funding; Clovis: Research Funding; Eisai: Research Funding. Damm: Astra Zeneca: Honoraria; AbbVie: Honoraria; Novartis: Honoraria; Roche: Honoraria; Gilead: Honoraria; Incyte: Honoraria.
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