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3498 Disparities in Microbiota Diversity Impacting Non-European Recipients of 8/8 HLA-Matched Unrelated Donor Transplantation

Program: Oral and Poster Abstracts
Session: 721. Allogeneic Transplantation: Conditioning Regimens, Engraftment, and Acute Toxicities: Poster II
Hematology Disease Topics & Pathways:
Research, Translational Research, Health outcomes research, Clinical Research, Health disparities research, Diversity, Equity, and Inclusion (DEI)
Sunday, December 8, 2024, 6:00 PM-8:00 PM

Natalie Grier Smith, BA1,2*, Warren B. Fingrut, MD2,3*, Nicholas Waters, PhD4*, Katarina Piasevoli5*, Teng Fei, PhD6*, John Slingerland5*, Jonathan U. Peled, MD, PhD2,7 and Doris M. Ponce, MD, MS2,7,8

1Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, Los Angeles, CA
2Equal Contribution, New York, NY
3Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas MD Anderson Cancer Center, Houston, TX
4Department of Immunology, Memorial Sloan Kettering Cancer Center, New York, NY
5Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY
6Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY
7Adult Bone Marrow Transplantation Service, Memorial Sloan Kettering Cancer Center, New York, NY
8Weill Cornell Medical College, New York, NY

Introduction. Although microbiome diversity, patient ancestry, and socioeconomic status (SES) are each associated with allogeneic hematopoietic cell transplantation (allo-HCT) outcomes, the relationships between these variables in the alloHCT context has not been described. We hypothesized that patient ancestry and SES associates with microbiome patterns post-HCT.

Methods. We evaluated alloHCT recipients at our Center 1/2015-6/2020 who provided ≥1 stool sample from D-40 to D+365. Patient SES was classified as low if they had an area deprivation index ≥60% or were on medicaid or patient financial assistance. Microbial diversity was examined at 4 time points: pre-HCT (D-40 to D-10), engraftment (D+10 to D+19), acute GVHD onset (aGVHD, +/- 7 days), and chronic GVHD landmark (cGVHD, D+100 to D+365). To examine microbial diversity, stool samples underwent 16S V4-V5 region amplification and sequencing, with diversity computed using Simpson’s reciprocal (S) for the most representative samples per patient in each timepoint. Butyrate producing anaerobes and strict vs facultative anaerobe ratios were measured. General estimating equations (GEE) modeling was used to investigate interactions between donor type with ancestry and SES. A FLORAL model was used to estimate taxonomic level markers and compare log ratios with a Wilcoxon test.

Results. Overall, 535 patients providing 6017 stool samples were included (median age 61 years, range 50-67; 51% with acute leukemia). 377 (70%) had European and 158 (30%) non-European ancestry (51 African, 40 non-Black Hispanic, 38 Asian, 5 Middle Eastern, 24 mixed non-European). 110 received HLA-matched sibling, 232 8/8 matched unrelated (URD) and 193 HLA-disparate grafts (81 cord blood, 78 haploidentical, 34 ≤7/8 unrelated donors). 151 (28%) received myeloablative and 384 (72%) non-myeloablative/ reduced-intensity HCT. Compared with Europeans, non-Europeans were younger (median 58 vs 65 years, p=.019) and received fewer 8/8 URD grafts (33/158, 21% vs 199/377, 53% p<.001).

≥ 1 stool sample was provided by 54 patients (32 European, 22 non-European) pre-HCT and by 371 (259 European, 112 non-European) at engraftment, 138 (89 European, 49 non-European) at aGVHD, and 206 (138 European, 68 non-European) at cGVHD timepoints.

Pre-HCT, there were no differences in microbiome diversity by patient ancestry.

Using GEE modeling, considering all timepoints, compared with Europeans, non-European 8/8 URD recipients had lower alpha-diversity (p=.007), whereas alpha-diversity was similar by ancestry for all other donor types.

Next we analyzed microbial diversity in 8/8 URD recipients by timepoint. At engraftment, compared with Europeans, non-Europeans had lower alpha diversity (p = 0.0432) and lower strict vs facultative anaerobe ratio (p < .001) but no difference in butyrate producing anaerobes. When observing taxonomic markers at engraftment by ancestry, non-Europeans had a lower ratio of favorable microbial bacteria including Lactobacillus/Scardovia ( p =.005) and Akkermansia (p = 0.0018), and a higher abundance of the unfavorable Escherichia/Phascolarctobacterium (p = 0.0023). At aGVHD and cGVHD timepoints, there were no differences in alpha diversity by patient ancestry. However, at aGVHD, compared with Europeans, non-Europeans had lower strict versus facultative anaerobe ratio (p = 0.0156). Non-Europeans also had decreased butyrate producing anaerobes at both the aGVHD (p<.001) and cGVHD (p=.0175) timepoints.

There were no differences in microbiome composition between groups by SES at any time point.

Conclusions. Our analyses identified disparities in microbiome diversity across multiple timepoints post-HCT which impact non-European ancestry 8/8 URD recipients. Prospective research is needed to investigate potential causes for these differences, considering antibiotic exposure, diet/lifestyle factors, and more detailed SES evaluation.

Disclosures: Peled: Seres Therapeutics: Patents & Royalties, Research Funding; DaVolterra: Consultancy; Crestone Inc: Consultancy; CSL Behring: Consultancy; Canaccord Genuity, Inc: Consultancy; MaaT Pharma: Consultancy; Postbiotics Plus Research: Current equity holder in publicly-traded company, Membership on an entity's Board of Directors or advisory committees; Prodigy Biosciences: Current equity holder in publicly-traded company, Membership on an entity's Board of Directors or advisory committees. Ponce: Evive: Consultancy; Sanofi: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Incyte: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; OncLive: Consultancy.

*signifies non-member of ASH