Session: 613. Acute Lymphoblastic Leukemias: Therapies Excluding Allogeneic Transplantation: Poster III
Methods: Adult patients in morphologic remission with detectable MRD (due to failure of MRD response or due to MRD relapse) were eligible. Patients in complete remission (CR) with MRD ≥1x10-4 after ≥3 months from frontline therapy or one month from salvage therapy were enrolled in this phase II trial. In patients with Philadelphia chromosome (Ph)-negative ALL, MRD positivity was defined by at least 1x10-4 by multiparameter flow cytometry (MFC) and/or any level by next-generation sequencing (NGS) for IG/TR. In patients with Ph-positive ALL, MRD positivity was defined as a ratio of BCR::ABL1 to ABL1 by polymerase chain reaction (PCR) of ≥0.01% or detectable MRD at a level of at least 1x10-4 by MFC and/or any level by NGS for IG/TR. INO was administered at 0.6 mg/m2 on Day 1 and 0.3 mg/m2 on Day 8 of Cycle 1, and 0.3 mg/m2 on Days 1 and 8 of Cycles 2-6. For patients with Ph-negative ALL, MRD negativity was defined as undetectable MRD by MFC with a sensitivity of ≥ 1x10-4 For patients with Ph-positive ALL, MRD negativity was defined as undetectable MRD by MFC with a sensitivity of ≥ 1x10-4 and the absence of quantifiable BCR::ABL1 transcripts by PCR with a sensitivity of ≥1 x10-5. Furthermore, MRD negativity by NGS was defined as undetectable MRD with a sensitivity of ≥ 1x10-6 when applicable All patients received ursodiol for hepatic sinusoidal obstruction syndrome (SOS) prophylaxis. Patients with Ph-positive ALL received a concomitant BCR::ABL1 tyrosine kinase inhibitor. MRD negativity was evaluated throughout induction and consolidation therapy using 6-color MFC and/or PCR. The primary efficacy endpoint was relapse-free survival (RFS).
Results: Thirty patients with a median age of 46 years (range, 19-70) were treated; 15 (50%) were males. Twenty-two (73%) were in CR1 and eight (27%) in CR2+; 17 (57%) had Ph-positive ALL. Seventeen patients (57%) had received prior blinatumomab. A median of three cycles (range, 1-6) were administered. Twenty patients (67%) responded and became MRD negative. In patients with Ph-negative ALL and positive MRD by MFC, the MRD negativity rate was 82% (9/11) with a median time to MRD negativity of 26 days (range, 22-44). In those with Ph-positive ALL, 65% (11/17) achieved CMR with a median time to CMR of 28 days (range, 20-51). The 2 patients with Ph-negative ALL and negative MRD by MFC but positive by NGS did not respond.
After a median follow-up of 36 months (range, 1-59), the 3-year OS rate was 56% and the RFS rate 42%. The 3-year OS rates were 60% versus 53%, and the 3-year RFS rates 50% versus 36%, in patients with Ph-negative ALL and Ph-positive ALL, respectively. Patients treated in CR1 had a 3-year OS of 63% compared with 38% in CR2+ (P=0.13).
The 3-year RFS rate was 40% in patients with baseline MRD ≥1x10-3 and 44% in those with MRD between 1x10-4 and 1x10-3 (P=0.97). Seven (35%) responders underwent stem cell transplantation, with no difference in survival compared with those who did not (p=0.75). Most adverse events were of low grade. SOS was seen in three patients: one pt with Ph-negative ALL developed SOS 2 weeks following transplant (recovered after defibrotide therapy), and two pts both with Ph-positive ALL on concurrent ponatinib (of whom one died).
Conclusion: INO resulted in high survival and MRD negativity rates in most patients with B-cell ALL and persistent MRD or MRD recurrence, with a favorable safety profile.
Disclosures: Kantarjian: AbbVie, Amgen, Ascentage, Ipsen Biopharmaceuticals, KAHR Medical, Novartis, Pfizer, Shenzhen Target Rx, Stemline,Takeda: Consultancy, Honoraria. Short: Sanofi: Honoraria; Autolus: Honoraria; Stemline Therapeutics: Research Funding; GSK: Consultancy, Research Funding; Novartis: Honoraria; Amgen: Honoraria; Adaptive Biotechnologies: Honoraria; Astellas Pharma, Inc.: Honoraria, Research Funding; Xencor: Research Funding; BeiGene: Honoraria; Takeda Oncology: Honoraria, Research Funding; Pfizer Inc.: Honoraria; NextCure: Research Funding. Jain: Loxo Oncology: Research Funding; Precision Biosciences: Consultancy, Honoraria, Other: Travel Support, Research Funding; ADC Therapeutics: Research Funding; Genentech: Consultancy, Honoraria, Other: Travel Support, Research Funding; Kite, a Gilead Company: Consultancy, Honoraria, Other: Travel Support, Research Funding; Ipsen: Consultancy, Honoraria, Other: Travel Support; CareDx: Consultancy, Honoraria, Other: Travel Support; MEI Pharma: Consultancy, Honoraria, Other: Travel Support; Bristol Myers Squibb: Consultancy, Honoraria, Other: Travel Support, Research Funding; Janssen: Consultancy, Honoraria, Other: Travel Support; MingSight: Honoraria, Research Funding; Cellectis: Consultancy, Honoraria, Other: Travel Support, Research Funding; Servier: Research Funding; Pharmacyclics: Consultancy, Honoraria, Other: Travel Support, Research Funding; TG Therapeutics: Consultancy, Honoraria, Other: Travel Support; Aprea Therapeutics: Research Funding; Dialectic Therapeutics: Research Funding; Fate Therapeutics: Research Funding; Incyte: Research Funding; Medisix: Research Funding; Pfizer: Research Funding; Newave: Research Funding; NovalGen: Research Funding; Takeda: Research Funding; TransThera Sciences: Research Funding; BeiGene: Consultancy, Honoraria, Other: Travel Support; AstraZeneca: Consultancy, Honoraria, Other: Travel Support, Research Funding; Adaptive Biotechnologies: Consultancy, Honoraria, Other: Travel Support, Research Funding; AbbVie: Consultancy, Honoraria, Other: Travel Support, Research Funding. Sasaki: Enliven: Research Funding; Novartis: Consultancy, Research Funding; Chugai: Other: Lecture fees; Otsuka: Other: Lecture fees; Pfizer: Consultancy; Daiichi-Sankyo: Consultancy. Burger: Novartis: Honoraria, Other: Travel, Accommodations, Expenses; Janssen: Honoraria, Other: Travel, Accommodations, Expenses, Speakers Bureau; Janssen: Consultancy; Pharmacyclics LLC, an AbbVie Company: Consultancy, Honoraria, Other: Travel, Accommodations, Expenses, Research Funding, Speakers Bureau; BeiGene: Consultancy, Research Funding, Speakers Bureau; TG Therapeutics: Consultancy, Honoraria, Other: Travel, Accommodations, Expenses, Speakers Bureau; Gilead: Consultancy, Honoraria, Other: Travel, Accommodations, Expenses, Speakers Bureau; AstraZeneca: Research Funding. DiNardo: Immunogen: Honoraria; Abbvie: Consultancy, Honoraria, Research Funding; GenMab: Consultancy, Honoraria, Other: data safety board; Notable Labs: Honoraria; Schrodinger: Consultancy, Honoraria; Amgen: Consultancy; Foghorn: Research Funding; Astex: Research Funding; Cleave: Research Funding; Servier: Consultancy, Honoraria, Other: meetingsupport, Research Funding; Riegel: Honoraria; ImmuneOnc: Research Funding; Loxo: Research Funding; Genetech: Honoraria; BMS: Consultancy, Honoraria, Research Funding; AstraZeneca: Honoraria; Astellas: Consultancy, Honoraria; Jazz: Consultancy, Honoraria; Rigel: Research Funding; Gilead: Consultancy; GSK: Consultancy, Honoraria; Stemline: Consultancy. Masarova: PharmaEssentia: Other: Advisory Board Participant; GSK: Consultancy, Other: Travel support; Cogent: Other: Advisory Board Participant; MorphoSys: Other: Advisory Board Participant. Kadia: Amgen: Research Funding; ASTEX: Research Funding; Sellas: Consultancy, Research Funding; BMS: Consultancy, Research Funding; Novartis: Honoraria; JAZZ: Research Funding; Servier: Consultancy; Rigel: Honoraria; Ascentage: Research Funding; Incyte: Research Funding; Pfizer: Research Funding; DrenBio: Consultancy, Research Funding; Regeneron: Research Funding; Abbvie: Consultancy, Research Funding; Genentech: Consultancy, Research Funding; AstraZeneca: Research Funding; Cellenkos: Research Funding. Garcia-Manero: Forty Seven: Research Funding; H3 Biomedicine: Research Funding; Genentech: Research Funding; Helsinn: Other: Personal fees; Novartis: Research Funding; AbbVie: Research Funding; Janssen: Research Funding; Helsinn: Research Funding; Amphivena: Research Funding; Genentech: Other: Personal fees; Merck: Research Funding; Astex: Research Funding; Curis: Research Funding; Astex: Other: Personal fees; Aprea: Research Funding; Bristol Myers Squibb: Other: Personal fees, Research Funding; Onconova: Research Funding. Ravandi: Prelude: Consultancy, Honoraria, Research Funding; Abbvie: Consultancy, Honoraria; Astellas: Consultancy, Honoraria; Xencor: Research Funding; Amgen: Research Funding; Syros: Consultancy, Honoraria, Research Funding; Syndax: Honoraria; BMS: Consultancy, Honoraria; Astyex/Taiho: Research Funding. Jabbour: AbbVie, Adaptive Biotechnologies, Amgen, Ascentage Pharma Group, Pfizer, Takeda: Research Funding; AbbVie, Adaptive Biotechnologies, Amgen, Astellas Pharma, BMS, Genentech, Incyte, Pfizer, Takeda: Consultancy.