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3539 Protein Language Model-Based Intra-Gene Disparity of Donor HLA Alleles Informs Prognostic Risk in Hematologic Malignancy Patients Undergoing Haplo-Identical HSCT: A Combined Xinqiao-CIBMTR Analysis

Program: Oral and Poster Abstracts
Session: 723. Allogeneic Transplantation: Long-term Follow-up, Complications, and Disease Recurrence: Poster II
Hematology Disease Topics & Pathways:
Research, Lymphoid Leukemias, ALL, Acute Myeloid Malignancies, AML, Artificial intelligence (AI), Clinical Research, Bioinformatics, Health disparities research, Diseases, Real-world evidence, Immunology, Lymphoid Malignancies, Survivorship, Myeloid Malignancies, Biological Processes, Emerging technologies, Technology and Procedures, Study Population, Human
Sunday, December 8, 2024, 6:00 PM-8:00 PM

Yimei Feng1*, Yijie Wang, PhD2*, Xiaobo Li2*, Da Di, PhD3*, Xiangjun Liu, PhD2* and Xi Zhang, PhD1

1Medical Center of Hematology, Xinqiao Hospital of Army Medical University, Chongqin, China
2Beijing BFR Gene Diagnostics LTD., Beijing, China
3Department of Genetics and Evolution–Anthropology Unit, University of Geneva, Geneva, Switzerland

It has been suggested that the intra-gene disparity in human leukocyte antigen (HLA) within a donor or recipient may play an important immunological role in haplo-identical hematopoietic stem cell transplantation (haplo-HSCT). Current methods to estimate HLA disparity primarily rely on epitope analysis and amino acid index scores. Recently, AlphaFold and emerging protein language models (PLMs) have highlighted the potential of large-scale deep learning techniques for predicting protein structures and functions. In this study, we utilized the newly proposed Protein-Vec PLM (PMID: 38045331) to extract functional embedding vectors from the amino acid sequences encoded by HLA alleles. With these vectors, we defined a novel PLM-based measure of intra-gene disparity for any allele pair of the same HLA gene within an individual and explored its prognostic value in haplo-HSCT.

We collected data from 425 hematologic malignancy (HM) patients undergoing haplo-HSCT between 2015 and 2022 at Xinqiao Hospital in Chongqing, China. We analyzed the association of PLM-based intra-gene HLA disparity (HLA-A/B/C/DRB1/DQB1) in a donor or recipient with overall survival (OS), disease-free survival (DFS), non-relapse mortality (NRM), and relapse. We validated our results by using a publicly available dataset from CIBMTR IB20-02 (PMID: 34774819), which included 985 HM patients who underwent haplo-HSCT between 2013 and 2017.

Using Cox regression to calculate proportional hazard ratios (HR), with p<0.01 as the threshold for statistical significance, our analysis of the Xinqiao dataset showed that higher disparity of donor HLA-B alleles was significantly associated with worse OS (HR=2.0, 95%CI 1.34-3.0, p=0.0007), worse DFS (HR=1.78, 95%CI 1.27-2.49, p=0.0008), higher NRM (HR=1.77, 95%CI 1.1-2.86, p=0.019), and higher relapse risk (HR=1.78, 95%CI 1.11-2.86, p=0.017). The result was further verified by the CIBMTR dataset, where higher disparity of donor HLA-B alleles was associated with worse OS (HR=1.3, 95%CI 1.09-1.57, p=0.0046), worse DFS (HR=1.20, 95%CI 1.02-1.42, p=0.03), higher NRM (HR=1.33, 95%CI 1.0-1.78, p=0.053), higher relapse risk (HR=1.14, 95%CI 0.93-1.40, p=0.2). Multivariate analysis demonstrated that PLM-based disparity of donor HLA-B alleles was an independent prognostic factor for OS in both Xinqiao and CIBMTR dataset. In contrast, we did not find significant results for intra-gene disparity of HLA-A/C/DRB1/DQB1 alleles in either donors or recipients.

In summary, PLM-based disparity of donor HLA-B alleles might be a potential prognostic factor in haplo-HSCT, and might be used as a novel tool assisting donor selection.

Disclosures: No relevant conflicts of interest to declare.

*signifies non-member of ASH