Type: Oral
Session: 636. Myelodysplastic Syndromes: Basic and Translational: Disease Mechanisms and Therapeutic Vulnerabilities in Molecular Genetic Subtypes of MDS
Hematology Disease Topics & Pathways:
Research, Fundamental Science, Translational Research, CHIP, Diseases, Myeloid Malignancies, Biological Processes
To elucidate the clonal origin and evolutionary trajectory of t-MNs post-ASCT, we studied 9 patients with MM who developed t-MNs following ASCT, with a median latency of 3 years (range: 1-8). We generated single-cell derived colonies from mobilized PBSCs of these patients and performed whole-genome sequencing (WGS) on these. A median of 86 colonies per patient was sequenced, totaling 1,032 colonies. For the matched t-MN samples, we conducted bulk WGS on bone marrow samples with a median coverage of 50x. Utilizing genome-wide somatic mutations identified in each colony, we constructed phylogenetic trees of PBSCs. Subsequently, we integrated the genome of t-MN samples within the PBSC phylogeny to identify the clonal origin (i.e., most recent common ancestor [MRCA]) of t-MNs at the single stem cell resolution.
In the phylogenetic trees derived from PBSC samples, parallel evolution of distinct TP53 and PPM1D mutations was pervasive. Single-cell WGS data facilitated clone-specific analyses of mutation burden and mutation signatures. The number of somatic mutations was comparable among TP53, PPM1D, and wild-type colonies. Cells harboring TP53 or PPM1D mutations did not exhibit specific mutation signatures. Among 84 TP53-mutated cells, 82 (97.6%) exhibited normal copy number profiles, with only 2 cells displaying copy number alterations in 17p. These findings suggest that TP53-mutated cells do not yet manifest genomic instability at the clonal hematopoiesis stage.
Integrated phylogenetic analysis of PBSC colonies and t-MN genomes identified the MRCA of t-MNs in 5 of 9 (56%) patients' PBSC samples. In these patients, the origin of t-MNs could be traced to a single stem cell carrying TP53 mutations, which later acquired chromosomal abnormalities or biallelic alteration of TP53 at the time of transformation. The MRCA was not identifiable in the PBSCs of 4 patients. In two of these patients, melphalan-related mutation signatures were detected in their t-MN samples. Since these patients did not receive melphalan therapy other than during ASCT conditioning, the presence of melphalan-related signatures in t-MN samples suggests that their clonal origin lies in bone marrow HSCs that were not mobilized.
Our findings indicate the existence of two distinct pathways for t-MN development post-ASCT: one originating from mutant stem cells in mobilized PBSCs, and the other from bone marrow stem cells that were not mobilized. Further studies are warranted to elucidate the clinical implications of these distinct evolutionary pathways in t-MNs.
Disclosures: Nangalia: Astra Zeneca: Consultancy, Speakers Bureau; Bioskryb: Consultancy. DiNardo: Servier: Consultancy, Honoraria, Other: meetingsupport, Research Funding; Abbvie: Consultancy, Honoraria, Research Funding; AstraZeneca: Honoraria; GSK: Consultancy, Honoraria; Immunogen: Honoraria; BMS: Consultancy, Honoraria, Research Funding; Rigel: Research Funding; Loxo: Research Funding; Astellas: Consultancy, Honoraria; Cleave: Research Funding; Schrodinger: Consultancy, Honoraria; ImmuneOnc: Research Funding; Astex: Research Funding; Notable Labs: Honoraria; Stemline: Consultancy; Amgen: Consultancy; Riegel: Honoraria; Jazz: Consultancy, Honoraria; GenMab: Consultancy, Honoraria, Other: data safety board; Genetech: Honoraria; Foghorn: Research Funding; Gilead: Consultancy. Daver: Menarini Group: Consultancy; Gilead: Consultancy, Research Funding; Novimmune: Research Funding; Trovagene: Research Funding; FATE Therapeutics: Other: Consulting Fees, Research Funding; Pfizer: Consultancy, Research Funding; Bristol Myers Squibb: Consultancy, Research Funding; Daiichi-Sankyo: Consultancy, Research Funding; Syndax: Consultancy; Shattuck Labs: Consultancy; Jazz: Consultancy; Genentech: Consultancy, Research Funding; Celgene: Consultancy; Astellas: Consultancy, Research Funding; Arog: Consultancy; Hanmi: Research Funding; Trillium: Consultancy, Research Funding; Novartis: Consultancy; Agios: Consultancy; Servier: Consultancy, Research Funding; KITE: Research Funding; Glycomimetics: Research Funding. Pemmaraju: Novartis: Honoraria, Research Funding; ClearView Healthcare Partners: Consultancy; Mustang Bio: Honoraria, Other: Travel Expenses, Research Funding; Protagonist Therapeutics: Consultancy; Roche Molecular Diagnostics: Honoraria; Stemline Therapeutics: Honoraria, Other: Travel Expenses, Research Funding; Affymetrix/Thermo Fisher Scientific: Research Funding; DAVA Oncology: Honoraria, Other: Travel Expenses; Pacylex: Consultancy; CareDx: Honoraria; Immunogen: Consultancy; Bristol-Myers Squibb: Consultancy; CTI BioPharma: Consultancy; LFB Biotechnologies: Honoraria; Celgene: Honoraria, Other: Travel Expenses; AbbVie: Honoraria, Other: Travel Expenses, Research Funding; Aptitude Health: Honoraria; Blueprint Medicines: Consultancy, Honoraria; Astellas: Consultancy; Cellectis: Research Funding; Springer Science + Business Media: Honoraria; Incyte: Honoraria; Triptych Health Partners: Consultancy; Neopharm: Honoraria; Daiichi Sankyo: Research Funding; Samus Therapeutics: Research Funding; Plexxikon: Research Funding; Blueprint Medicines OncLive PeerView Institute for Medical Education: Consultancy, Other: advisory board; ASH Committee on Communications ASCO Cancer.NET Editorial Board: Other: Leadership; Karger Publishers: Other: Licenses; National Institute of Health/National Cancer Institute (NIH/NCI): Research Funding; HemOnc Times/Oncology Times: Other: uncompensated. Jain: Janssen: Consultancy, Honoraria, Other: Travel Support; BeiGene: Consultancy, Honoraria, Other: Travel Support; Loxo Oncology: Research Funding; Kite, a Gilead Company: Consultancy, Honoraria, Other: Travel Support, Research Funding; MEI Pharma: Consultancy, Honoraria, Other: Travel Support; Pharmacyclics: Consultancy, Honoraria, Other: Travel Support, Research Funding; Precision Biosciences: Consultancy, Honoraria, Other: Travel Support, Research Funding; TG Therapeutics: Consultancy, Honoraria, Other: Travel Support; ADC Therapeutics: Research Funding; Aprea Therapeutics: Research Funding; Dialectic Therapeutics: Research Funding; Fate Therapeutics: Research Funding; Medisix: Research Funding; NovalGen: Research Funding; MingSight: Honoraria, Research Funding; Newave: Research Funding; Pfizer: Research Funding; Servier: Research Funding; Takeda: Research Funding; TransThera Sciences: Research Funding; Ipsen: Consultancy, Honoraria, Other: Travel Support; Genentech: Consultancy, Honoraria, Other: Travel Support, Research Funding; Incyte: Research Funding; Cellectis: Consultancy, Honoraria, Other: Travel Support, Research Funding; Bristol Myers Squibb: Consultancy, Honoraria, Other: Travel Support, Research Funding; AstraZeneca: Consultancy, Honoraria, Other: Travel Support, Research Funding; CareDx: Consultancy, Honoraria, Other: Travel Support; Adaptive Biotechnologies: Consultancy, Honoraria, Other: Travel Support, Research Funding; AbbVie: Consultancy, Honoraria, Other: Travel Support, Research Funding. Ravandi: Amgen: Research Funding; Xencor: Research Funding; Syndax: Honoraria; Prelude: Consultancy, Honoraria, Research Funding; Astellas: Consultancy, Honoraria; Abbvie: Consultancy, Honoraria; BMS: Consultancy, Honoraria; Syros: Consultancy, Honoraria, Research Funding; Astyex/Taiho: Research Funding. Orlowski: AbbVie Inc, Adaptive Biotechnologies Corporation, Asylia Therapeutics Inc, BioTheryX Inc, Bristol Myers Squibb, Karyopharm Therapeutics, Meridian Therapeutics, Monte Rosa Therapeutics, Nanjing IASO Biotherapeutics, Neoleukin Therapeutics, Oncopeptides, Pf: Membership on an entity's Board of Directors or advisory committees; Bristol Myers Squibb, CARsgen Therapeutics, Exelixis Inc, Heidelberg Pharma, Janssen Biotech Inc, Sanofi, Takeda Pharmaceuticals USA Inc; Laboratory Research Funding: Asylia Therapeutics Inc, BioTheryX Inc, Heidelberg Pharma: Research Funding; Asylia Therapeutics Inc.: Current equity holder in private company, Patents & Royalties; BioTheryX: Membership on an entity's Board of Directors or advisory committees, Research Funding; Bristol-Myers Squibb Pharmaceuticals: Membership on an entity's Board of Directors or advisory committees; DEM BioPharma, Inc., Karyopharm Therapeutics, Lytica Therapeutics, Meridian Therapeutics, Monte Rosa Therapeutics, Myeloma 360, Nanjing IASO Biotherapeutics, Neoleukin Corporation, Oncopeptides AB, Pfizer, Inc., Regeneron Pharmaceuticals, Inc., Sporos Bio: Membership on an entity's Board of Directors or advisory committees; Sanofi, Takeda Pharmaceuticals: Membership on an entity's Board of Directors or advisory committees, Research Funding. Qazilbash: NexImmune: Research Funding; Angiocrine Bioscience: Research Funding; BioLineRx: Research Funding; Amgen: Research Funding; Janssen Pharmaceuticals: Research Funding. Kantarjian: AbbVie, Amgen, Ascentage, Ipsen Biopharmaceuticals, KAHR Medical, Novartis, Pfizer, Shenzhen Target Rx, Stemline,Takeda: Consultancy, Honoraria. Shpall: FibroBiologics: Other: Scientific Advisor; National Marrow Donor Program: Other: Board of Directors/Management; Adaptimmune Limited: Other: Scientific Advisor; Zelluna Immunotherapy: Other: Scientific Advisor; Axio Research: Current Employment, Other: Scientific Advisor. Garcia-Manero: Forty Seven: Research Funding; Astex: Research Funding; AbbVie: Research Funding; Helsinn: Other: Personal fees; Merck: Research Funding; Astex: Other: Personal fees; Genentech: Research Funding; H3 Biomedicine: Research Funding; Onconova: Research Funding; Genentech: Other: Personal fees; Curis: Research Funding; Janssen: Research Funding; Amphivena: Research Funding; Novartis: Research Funding; Helsinn: Research Funding; Aprea: Research Funding; Bristol Myers Squibb: Other: Personal fees, Research Funding.