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4027 An Erythrocyte-Based Delivery Platform for Leukemia Treatment

Program: Oral and Poster Abstracts
Session: 401. Blood Transfusion: Poster III
Hematology Disease Topics & Pathways:
Research, Fundamental Science
Monday, December 9, 2024, 6:00 PM-8:00 PM

Mengran Chen, MD1*, Lei Zhao, MD2*, Ying Qu, PhD3* and Yu Wu, MD2

1Department of Hematology and Hematology Research Laboratory, West China Hospital,Sichuan Universtiy, Chengdu, China
2Department of Hematology and Hematology Research Laboratory, West China Hospital, Sichuan University, Chengdu, China
3department of Hematology and Hematology Research Laboratory, West China Hospital, Sichuan University, China, chengdu, sichuan, China

Background:Leukemia is a highly malignant hematologic neoplasm commonly treated with intensive chemotherapy as frontline therapy, albeit associated with significant side effects that may be poorly tolerated by elderly patients. Erythrocytes, the most abundant cells in blood, possess excellent biocompatibility and provide ample storage capacity due to their lack of organelles. Their biconcave disc morphology confers elastic properties beneficial for drug encapsulation. Utilizing erythrocyte as carrier can significantly extend drug half-life and address challenges in the in vivo distribution of water-soluble drugs across the blood-brain and bone marrow barriers, presenting numerous advantages in leukemia therapy. At present, there is still a lack of exploration on using intact erythrocyte to deliver chemotherapy drugs for leukemia treatment. Thus we designed an erythrocyte-based drug delivery system using intact and biologically active erythrocytes to encapsulate the chemotherapeutic agent Doxorubicin (E-Dox), with the goal of achieving enhanced therapeutic efficacy and reduced toxicity.

Methods:E-Dox was prepared using a hypotonic-hypertonic method. Characterization and release assays confirmed the successful preparation of E-Dox. Subsequent investigations evaluated the tumor inhibitory capacity, pharmacokinetics, and biodistribution in vivo. We also analyzed the immune environment in the bone marrow and spleen using flow cytometry and IHC staining. Furthermore, the safety profile of the system was validated.

Results:E-Dox preparation preserved the biconcave disc morphology, with minimal differences in cellular protein, notably CD47, compared to normal erythrocyte as determined by Western Blot analysis. Furthermore, the system exhibited high encapsulation efficiency, reaching 80%. E-Dox also demonstrated significant tumor inhibitory effects. Following treatment with E-Dox, there was an increase in early apoptotic cells among tumor cells and elevated production of reactive oxygen species within the tumor cells compared with free Dox. Importantly, E-Dox markedly prolonged drug half-life, achieving a four-fold extension compared to free drug formulations. Leukemia mice treated with E-Dox showed attenuated tumor progression and prolonged survival period. Interestingly, by analyzing the immune environment in the bone marrow and spleen, we observed an increase in activated T cells and cytotoxic T cells in mice treated with E-Dox. And Hepatic and renal function tests, along with histopathological analysis, indicated favorable biocompatibility of E-Dox and alleviated doxorubicin-induced cardiac toxicity.

Conclusion:This study elucidates an optimal strategy for preparing E-Dox, successfully creating an intact and functional erythrocyte-based delivery platform. The system significantly extends drug action duration, effectively suppresses leukemia in mouse models, and demonstrates biocompatibility, thereby mitigating chemotherapy-induced organ toxicity.

Disclosures: No relevant conflicts of interest to declare.

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*signifies non-member of ASH