Session: 900. Health Services and Quality Improvement: Hemoglobinopathies: Poster II
Hematology Disease Topics & Pathways:
Research, Sickle Cell Disease, Adult, Epidemiology, Clinical Practice (Health Services and Quality), Sickle Cell Trait, Assays, Clinical Research, Health outcomes research, Thalassemia, Health disparities research, Diversity, Equity, and Inclusion (DEI), Hemoglobinopathies, Supportive Care, Diseases, Real-world evidence, Treatment Considerations, Young adult , Emerging technologies, Technology and Procedures, Study Population, Human, Serologic Tests
Methods: The current study was conducted for the detection of abnormal hemoglobin variants using HPLC and microchip electrophoresis technology in two states estimated to have the highest prevalence of SCD in India.
Results: A total of 77 blood samples were processed for population surveillance in these previously unscreened regions and corroborated with HPLC. Out of 37 samples from Odisha, 32 were diagnosed with hemoglobinopathies where 14 cases had sickle cell disease, 7 cases with sickle cell trait, 6 cases with β-thalassemia trait, 4 were diagnosed as β-thalassemia major, and 1 case of HbE disease. Out of 44 samples from West Bengal, 32 were diagnosed with hemoglobinopathies. Among them, 1 case of β-thalassemia major, 8 cases of β-thalassemia trait, 3 cases of HbE disease, 7 cases of HbE trait, 1 case of sickle beta disease, 2 cases of sickle cell anemia (SCA) disease, 1 case of sickle cell trait and 8 cases of HbE β-thalassemia were observed. The results showed 99% concordance for both the testing methods. When compared to HPLC, microchip capillary electrophoresis showed 100% sensitivity, specificity, and accuracy for controls vs disease; 96% sensitivity, 97.78% specificity and 97.14% accuracy for disease vs trait; and 96.55% sensitivity, 97.56% specificity and 97.14% accuracy for trait vs disease (p =0.016).
Conclusion: Our study in a global health LMIC setting demonstrates that the microchip electrophoresis-based technology enables affordable and easy identification of common Hb variants with excellent accuracy in the field at the point of need with the same accuracy as HPLC. It is a adaptable platform for accurate diagnostic testing of SCDs and other hemoglobinopathies in low-resource regions where reliable electricity, lack of trained manpower and high cost are always prohibitive for phlebotomy-based community screening and SCD testing.
Disclosures: Jain: Blue Bird Bio: Other: End point adjudication committee ; Beam Therapeutics: Other: End point adjudication committee; NovoNordisk: Speakers Bureau; Sanofi: Speakers Bureau; Hemex Health: Consultancy. Thota: Hemex Health Inc: Current Employment.
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