Session: 102. Iron Homeostasis and Biology: Poster I
Hematology Disease Topics & Pathways:
Biological Processes
The aim of this study was to evaluate ZPP as a bona fide marker of iron deficiency in MPN. Between September 2020 and June 2024 blood samples of MPN patients were analyzed. Data from 233 MPN patients and a cohort of 4420 individuals without diagnosis of MPN from the Study of Health In Pomerania (SHIP; SHIP-TREND-0, 2008-2012) were analyzed. Regarding the MPN diagnoses, 93 (40%) had been diagnosed with polycythemia vera (PV), 36 (15%) with essential thrombocythemia, 87 (37%) with myelofibrosis, and 17 (7%) with unclassifiable MPN. For quantitative ZPP measurement, fluorescence spectroscopy-based FIDscreen (prototype device, FerroSens GmbH) was used. ZPP levels < 40 µmol/mol heme were considered normal, 40-60 µmol/mol as indicator of mild, >60 µmol/mol as manifest, and >100 µmol/mol as severe iron deficiency. Standard descriptive statistics were used to describe the data. Associations between two continuous variables were determined using Pearson’s correlation coefficient.
In the control SHIP-study cohort a positive correlation between hemoglobin (HGB) and ferritin (Pearson correlation coefficient R = 0.27 (CI: 0.24, 0.29), p < 0.001) could be shown. In MPN patients, however, the correlation between HGB and ferritin was negative (R = -0.50 (CI: -0.63, -0.33), p < 0.001), thus limiting the use of ferritin as indicator for iron deficiency. One third of MPN patients had ZPP levels below 40 µmol/mol heme. ZPP levels were elevated in all MPN subgroups and values above 100 µmol/mol heme, indicating severe iron deficiency, were found in 45/233 (19%) patients. The highest ZPP levels were found in PV patients, where 25% (23/93) of patients had ZPP levels of 100 µmol/mol heme or higher. Data on phlebotomy-dependence were available for 53 patients with PV. In this subset of PV patients, mean ZPP values were significantly higher in patients who underwent phlebotomies than in patients who did not (101 vs. 64 µmol/mol heme, p = 0.035). Consistently, ZPP and HGB were negatively associated in MPN patients (R = -0.29 (CI: -0.42, -0.14), p < 0.001), whereas ZPP and ferritin did not show an association (R = -0.04 (CI: -0.23, 0.16), p = 0.709).
We report on the suitability of ZPP as a reliable marker of iron deficiency in MPN patients. In contrast to the general population, ferritin is not a suitable iron deficiency marker in MPN patients, as many MPN patients may have elevated ferritin levels due to inflammatory processes. ZPP can therefore detect functional iron deficiency in MPN patients, which correlates closely with hemoglobin levels.
Disclosures: Al-Ali: Stemline: Honoraria; Blueprint: Consultancy, Honoraria; Blueprint: Honoraria; Incyte: Research Funding; Otsuka: Consultancy, Honoraria; AOP: Consultancy, Honoraria; GSK: Consultancy, Honoraria; Abbvie: Consultancy, Honoraria, Other: Travel grant; BMS: Consultancy, Research Funding; MSD: Honoraria; Alexion: Other: Travel grant; Novartis: Consultancy, Honoraria. Isfort: AOP Orphan: Honoraria, Other: Travel support; Hexal: Other: Travel support; Mundipharma: Other: Travel support; Incyte: Honoraria; Pfizer: Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Travel support; Silence Therapeutics: Membership on an entity's Board of Directors or advisory committees; Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Travel support; GSK: Honoraria, Membership on an entity's Board of Directors or advisory committees; Roche: Other: Travel support; Alexion: Other: Travel support. Thol: Rigel: Membership on an entity's Board of Directors or advisory committees; Astellas: Honoraria; Menarini: Membership on an entity's Board of Directors or advisory committees; AbbVie: Honoraria, Membership on an entity's Board of Directors or advisory committees; Novartis: Membership on an entity's Board of Directors or advisory committees; BMS: Membership on an entity's Board of Directors or advisory committees. Stauch: Novartis: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Amgen: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Sobi: Research Funding, Speakers Bureau; Argenx: Research Funding; Grifols: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Alexion: Speakers Bureau; Janssen: Speakers Bureau; AOP: Speakers Bureau; AstraZeneca: Speakers Bureau; Celgene/BMS: Other. Hoffmann: Pfizer: Honoraria. Hochhaus: Novartis: Honoraria, Other: Advisory Board, Research Funding; Enliven: Honoraria, Other: Advisory Board; Terns: Honoraria, Other: Advisory Board. Palandri: AOP: Consultancy, Honoraria; BMS/Celgene: Consultancy, Honoraria; Constellation-Morphosys: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Sobi: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Sierra Oncology: Consultancy, Honoraria; Telios: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Abbvie: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; CTI: Consultancy, Honoraria; Incyte: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Novartis: Consultancy, Honoraria. Homann: FerroSens GmbH: Current Employment, Current equity holder in private company. Heidel: BMS/Celgene, Novartis, CTI: Research Funding; BMS/Celgene, AOP, Novartis, CTI, Janssen, Abbvie, GSK, Merck, Kartos, Telios: Consultancy.