Type: Oral
Session: 637. Myelodysplastic Syndromes: Clinical and Epidemiological: Defining and Treating Chronic Myelomonocytic Leukemia
Hematology Disease Topics & Pathways:
Research, Acute Myeloid Malignancies, AML, MDS, Artificial intelligence (AI), Adult, Elderly, Clinical Practice (Health Services and Quality), Translational Research, APL, MPN, Clinical Research, Genomics, Chronic Myeloid Malignancies, CMML, Diseases, Real-world evidence, Myeloid Malignancies, Biological Processes, Molecular biology, Technology and Procedures, Study Population, Human, Machine learning, Omics technologies, Pathology
Methods. In the TITAN study, we retrospectively studied 20,054 patients with MN in which clinical and morphological data, together with cytogenetics, mutational screening and outcome were available. We included 7104 AML, 8410 MDS, 2986 MDS/MPN and 1554 MF. We used MOSAIC, an AI-based framework to define unsupervised clusters according to homogeneous morphological and genomic features. Shapley Additive exPlanations (SHAP) was applied to investigate features of importance and their effects on cluster assignment
Results. We identified 34 distinct clusters and created a dashboard (https://titan-xkb3corsxq-ew.a.run.app/) where the most relevant features, clinical phenotypes, and outcomes are summarized. SHAP revealed that genomics often outweighs morphology in defining disease entities
Six clusters had splicing mutations as dominant features, albeit with different hematological phenotypes (mainly including MDS and MDS/MPN pts). The presence of additional high-risk genomic features (RUNX1/ASXL1 mutations, del(7q)/-7, abn(3q26.2) and complex karyotype) identified patients with poor survival within each category. A separate cluster including MN with splicing mutations and increased blasts (mainly including MDS and AML patients) was characterized by poor response to treatments and dismal outcome
MN with TP53 mutations constituted a distinct entity, regardless of clinical phenotype and blast count and had the poorest prognosis. Mutational and CNV analysis showed biallelic inactivation of TP53 in the majority of patients. Most monoallelic TP53 were assigned to a different cluster. Patients that evolved to AML often acquired biallelic inactivation, suggesting that TP53 allelic status may identify different disease phases within the same entity
Among morphological features, marrow fibrosis held significant hierarchical importance in defining clusters, driving patient assignment into three distinct groups characterized by 1) JAK/STAT mutations alone, 2) JAK/STAT mutations with high-risk molecular features, 3) wildtype JAK/STAT. The latter group included both MDS and MF patients who exhibited a higher risk of clonal evolution and shorter survival
Overall, 10 out of 34 clusters included patients from different MN subtypes as defined by ICC/WHO criteria, accounting for 38.1% of the entire population. This suggests that current classifications do not efficiently capture boundaries between different entities. Importantly, among 1482 patients with RNAseq data from BM progenitors available, the newly identified clusters exhibited distinct gene expression profiles, thus providing evidence for the biological consistency of the proposed classification
The 34 clusters were characterized by different risks of clonal progression and survival. Based on this finding and the observation that, in most cases, heterogeneous phenotypes contributed to each cluster, we developed a pan-MN prognostic score. This score is based on clinical and molecular parameters and is designed to be applicable to all patients, regardless of clinical labels. The performance of the score (c-index 0.75) was comparable to that of currently available disease-specific tools
Conclusion. The integrative analysis of genomic profiling and morphological features provides a proof of concept for a novel classification approach for MN, that may refine the overlapping areas among disease entities and more effectively identify patients with a homogeneous biological background. It could serve as a foundation for an innovative prognostic assessment of MN patients, independent of traditional clinical labels. The clinical implementation of pan-MN tools is expected to improve the effectiveness and quality of personalized diagnosis, treatment decisions and clinical trial eligibility
Disclosures: Gagelmann: J&J: Honoraria, Other: Travel support; BMS: Honoraria. Guglielmelli: AOP: Honoraria; Abbvie: Honoraria, Membership on an entity's Board of Directors or advisory committees; GSK: Honoraria, Membership on an entity's Board of Directors or advisory committees; Incyte: Honoraria, Membership on an entity's Board of Directors or advisory committees; Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees. Hunter: GSK: Consultancy, Honoraria; PharmaEssentia: Consultancy, Honoraria; Blueprint Medicines: Consultancy, Honoraria, Research Funding; Sobi (formerly CTI biopharma): Consultancy, Honoraria; Incyte: Consultancy, Honoraria, Research Funding; Cogent Biosciences: Research Funding; Ascentage Pharma: Research Funding; Syntrix Biosystems: Research Funding; Novartis: Research Funding; PharmaEssentia: Research Funding. Kubasch: Novartis: Honoraria, Research Funding; BMS: Honoraria; Curis: Research Funding; Janssen: Honoraria, Research Funding. Santoro: Sandoz: Speakers Bureau; Novartis: Speakers Bureau; Beigene: Speakers Bureau; Arqule: Speakers Bureau; Astrazeneca: Speakers Bureau; Celgene: Speakers Bureau; Amgen: Speakers Bureau; Abb-vie: Speakers Bureau; Roche: Speakers Bureau; Takeda: Speakers Bureau; MSD: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Bayer: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; EISAI: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Pfizer: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Gilead: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Servier: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; BMS: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Incyte: Consultancy; Sanofi: Consultancy; Lilly: Speakers Bureau. Xicoy: BMS: Honoraria. Vucinic: Gilead/Kite, Janssen, BMS Celgene, Novartis: Consultancy, Honoraria; Amgen: Honoraria, Other: Travel grant. Sallman: Abbvie: Consultancy; Agios: Consultancy; Axiom: Consultancy; Gilead: Consultancy; Celyad: Consultancy; Froghorn: Consultancy; Incyte: Consultancy; Intellisphere, LLC: Consultancy; Johnson & Johnson: Consultancy; Kite: Consultancy, Membership on an entity's Board of Directors or advisory committees; Magenta Therapeutics: Consultancy, Membership on an entity's Board of Directors or advisory committees; NextTech: Consultancy; Novartis: Consultancy, Membership on an entity's Board of Directors or advisory committees; AvenCell: Membership on an entity's Board of Directors or advisory committees; Astellas: Membership on an entity's Board of Directors or advisory committees; BlueBird Bio: Membership on an entity's Board of Directors or advisory committees; BMS: Membership on an entity's Board of Directors or advisory committees; Dark Blue Therapeutics: Membership on an entity's Board of Directors or advisory committees; Intellia: Membership on an entity's Board of Directors or advisory committees; Jasper Therapeutics: Membership on an entity's Board of Directors or advisory committees; NKARTA: Membership on an entity's Board of Directors or advisory committees; Orbital Therapeutics: Membership on an entity's Board of Directors or advisory committees; Rigel Pharmaceuticals: Membership on an entity's Board of Directors or advisory committees; Shattuck Labs: Membership on an entity's Board of Directors or advisory committees; Servier: Membership on an entity's Board of Directors or advisory committees; Syndax: Membership on an entity's Board of Directors or advisory committees; Syros: Membership on an entity's Board of Directors or advisory committees; Apera: Research Funding; Jazz: Research Funding. Voso: Astra Zeneca: Speakers Bureau; Abbvie: Speakers Bureau; Syros: Other: Advisory Board; Jazz: Other: Advisory Board, Speakers Bureau; Astellas: Speakers Bureau; Novartis: Other: Research support, Speakers Bureau; Celgene/BMS: Other: Research support, Advisory Board, Speakers Bureau. Pleyer: BMS: Honoraria; AbbVie: Honoraria; Otsuka: Honoraria. Jerez: Astrazeneca: Research Funding. Díaz-Beyá: BMS: Consultancy; Abbvie: Consultancy; Takeda: Consultancy; Novartis: Consultancy; Jazz Pharmaceuticals: Consultancy. Garcia-Manero: Novartis: Research Funding; Onconova: Research Funding; Helsinn: Other: Personal fees; Aprea: Research Funding; Astex: Research Funding; AbbVie: Research Funding; Genentech: Other: Personal fees; Astex: Other: Personal fees; Amphivena: Research Funding; H3 Biomedicine: Research Funding; Curis: Research Funding; Janssen: Research Funding; Genentech: Research Funding; Forty Seven: Research Funding; Helsinn: Research Funding; Bristol Myers Squibb: Other: Personal fees, Research Funding; Merck: Research Funding. Loghavi: Pathology Education Partners; VJ HemeOnc, College of American Pathologists, OncLive, ICCS, MD Education, NCCN, MashUp Media, NCTN, Aptitude Health: Honoraria; Guidepoint; QualWorld; Gerson Lehrman Group, AlphaSight, Arima, Qiagen, Opinion Health: Consultancy; Astellas, Amgen: Research Funding; Abbvie: Current holder of stock options in a privately-held company; Syndx, Servier, BMS: Membership on an entity's Board of Directors or advisory committees; Abbvie, Daiichi Sankyo, BluePrint Medicine, Caris Diagnostics, Recordati, Servier: Consultancy. Platzbecker: Amgen: Consultancy, Research Funding; BMS: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: Travel support, Research Funding; MDS Foundation: Membership on an entity's Board of Directors or advisory committees; Abbvie: Consultancy, Research Funding; Curis: Consultancy, Honoraria, Research Funding; Geron: Consultancy; Janssen: Consultancy, Honoraria, Research Funding; Merck: Research Funding; Novartis: Consultancy, Research Funding. Diez-Campelo: KEROS: Honoraria, Membership on an entity's Board of Directors or advisory committees; AGIOS: Consultancy, Membership on an entity's Board of Directors or advisory committees; Gilead: Other: Travel reimbursement; SYROS: Membership on an entity's Board of Directors or advisory committees; BMS/Celgene: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Advisory board fees; BLUEPRINT MEDICINES: Consultancy, Membership on an entity's Board of Directors or advisory committees; Novartis: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; HEMAVAN: Membership on an entity's Board of Directors or advisory committees; ASTEX/OTSUKA: Membership on an entity's Board of Directors or advisory committees, Other: TRAVEL TO MEETINGS; CURIS: Membership on an entity's Board of Directors or advisory committees; GSK: Consultancy, Membership on an entity's Board of Directors or advisory committees. Kröger: Novartis, BMS, Neovii, Kite/Gilead, Sanofi, Takada: Membership on an entity's Board of Directors or advisory committees; Novartis, Neovii, Kite/Gilead, Therakos, Alexion, Sanofi, Takeda: Other: Speaker honoraria; Novartis, DKMS: Research Funding; Provirex: Consultancy. Fenaux: Servier: Research Funding; Janssen: Research Funding; AbbVie: Honoraria, Research Funding; Jazz Pharmaceuticals: Honoraria, Research Funding; Novartis: Research Funding; Astex: Research Funding; Agios: Research Funding; BMS: Honoraria, Research Funding. Santini: Ascentage, AbbVie, Bristol Myers Squibb, CTI BioPharma, Geron, Gilead, Novartis, Servier, Syros Pharmaceuticals: Other: Advisory Board. Germing: Novatis: Honoraria; BMS: Research Funding; Abbvie: Research Funding; JAZZ: Research Funding; BMS: Honoraria. Robin: Neovii: Other: research support; Novartis: Other: research support; Abbvie: Other: research support; Medac: Other: research support. Passamonti: Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees. Solary: PEPKON: Research Funding; NOVARTIS: Speakers Bureau. Vannucchi: AOP: Consultancy, Speakers Bureau; Blueprint: Consultancy, Speakers Bureau; GSK: Consultancy, Speakers Bureau; Novartis: Consultancy, Speakers Bureau; BMS: Consultancy, Speakers Bureau; Incyte: Consultancy, Speakers Bureau. Komrokji: Novartis: Membership on an entity's Board of Directors or advisory committees; CTI biopharma: Membership on an entity's Board of Directors or advisory committees; Servio: Honoraria; Taiho: Membership on an entity's Board of Directors or advisory committees; Janssen: Consultancy; BMS: Honoraria, Membership on an entity's Board of Directors or advisory committees; DSI: Honoraria, Membership on an entity's Board of Directors or advisory committees; PharmaEssentia: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Rigel: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Jazz Pharmaceuticals: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Geron: Consultancy, Membership on an entity's Board of Directors or advisory committees; Celgene/BMS: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Genentech: Consultancy; Keros: Membership on an entity's Board of Directors or advisory committees; DSI: Consultancy, Membership on an entity's Board of Directors or advisory committees; Sumitomo Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees; Sobi: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; AbbVie: Consultancy, Membership on an entity's Board of Directors or advisory committees; BMS: Research Funding; Servier: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Servio: Membership on an entity's Board of Directors or advisory committees. Della Porta: Bristol Myers Squibb: Consultancy.