-Author name in bold denotes the presenting author
-Asterisk * with author name denotes a Non-ASH member
Clinically Relevant Abstract denotes an abstract that is clinically relevant.

PhD Trainee denotes that this is a recommended PHD Trainee Session.

Ticketed Session denotes that this is a ticketed session.

1005 Artificial-Intelligence, Data-Driven, Comprehensive Classification of Myeloid Neoplasms Based on Genomic, Morphological and Histological FeaturesClinically Relevant Abstract

Program: Oral and Poster Abstracts
Type: Oral
Session: 637. Myelodysplastic Syndromes: Clinical and Epidemiological: Defining and Treating Chronic Myelomonocytic Leukemia
Hematology Disease Topics & Pathways:
Research, Acute Myeloid Malignancies, AML, MDS, Artificial intelligence (AI), Adult, Elderly, Clinical Practice (Health Services and Quality), Translational Research, APL, MPN, Clinical Research, Genomics, Chronic Myeloid Malignancies, CMML, Diseases, Real-world evidence, Myeloid Malignancies, Biological Processes, Molecular biology, Technology and Procedures, Study Population, Human, Machine learning, Omics technologies, Pathology
Monday, December 9, 2024: 5:00 PM

Luca Lanino, MD1,2, Saverio D'Amico, MSc3,4*, Giulia Maggioni, MD3*, Najla H. Al Ali, Ms5*, Yu-Hung Wang, MD6*, Carmelo Gurnari, MD, PhD7, Nico Gagelmann, MD8*, Jan Philipp Bewersdorf, MD9, Somedeb Ball, MD10, Paola Guglielmelli, MD11, Manja Meggendorfer, PhD12, Anthony M. Hunter, MD13, Anne Sophie Kubasch, MD14, Erica Travaglino, BS15*, Alessia Campagna, MD3*, Marta Ubezio, MD3*, Antonio Russo, MD1*, Gabriele Todisco, MD2*, Cristina Astrid Tentori, MD3*, Alessandro Buizza16*, Elisabetta Sauta, PhD3*, Matteo Zampini, PhD1*, Elena Riva, PhD1*, Gianluca Asti, MSc3*, Mattia Delleani3*, Francesca Ficara, PhD3*, Armando Santoro, MD16*, Claudia Sala, PhD17*, Daniele Dall'Olio, PhD18*, Lorenzo Dall'Olio, PhD19*, Tariq Kewan, MD20, Ilaria Carola Casetti, MD, PhD21*, Hussein Awada22, Blanca Xicoy, MD23*, Vladan Vucinic, MD24*, Hsin-An Hou, M.D.; Ph.D.25, Wen-Chien Chou, MD, PhD26, Chi-Yuan Yao, MD26*, Chien-Chin Lin, MD, PhD27*, Hwei-Fang Tien, MD, PhD28, Angela Consagra, MD29*, David Sallman, MD5, Wolfgang Kern, MD30, Massimo Bernardi, MD31*, Patrizia Chiusolo, MD, PhD32*, Lorenza Maria Borin, MD33*, Maria Teresa Voso, MD34, Lisa Pleyer, MD35*, Laura Palomo, PhD36*, David Quintela37*, Andres Jerez38*, Elena Cornejo39*, Paloma Garcia Martin39*, Marina Díaz-Beyá, MD, PhD40*, Alejandro Avendaño Pita41*, Veronica Roldan42*, Dolly Viviana Fiallo Suarez43*, Estefania Cerezo Velasco, MD44*, Marisa Calabuig45*, Guillermo Garcia-Manero, MD46, Sanam Loghavi, MD47, Uwe Platzbecker, MD48, Francesc Sole, PhD49, Maria Diez-Campelo, MD, PhD50*, Jaroslaw Maciejewski22, Nicolaus Kröger, MD Prof51*, Pierre Fenaux, MD52, Michaela Fontenay, MD, PhD53*, Valeria Santini, MD54, Torsten Haferlach, MD30, Ulrich Germing, MD55*, Eric Padron, MD5, Marie Robin, MD56*, Francesco Passamonti, MD57*, Eric Solary, MD58*, Alessandro M. Vannucchi, MD, PhD59, Gastone Castellani, PhD60*, Amer M. Zeidan, MD61, Rami S. Komrokji, MD62 and Matteo Giovanni Della Porta, MD2,3*

1IRCCS Humanitas Research Hospital, Rozzano, Milano, Italy
2Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy
3IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy
4Train s.r.l., Milan, Italy
5Department of Malignant Hematology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL
6Department of Internal Medicine, Fra-Eastern Memorial Hospital, Taipei, Taiwan
7Cleveland Clinic Foundation, Cleveland, OH
8Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany
9Memorial Sloan Kettering Cancer Center, New York, NY
10Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN
11Department of Experimental and Clinical Medicine, Centro di Ricerca e Innovazione Malattie Mieloproliferative (CRIMM), AOU Careggi, University of Florence, Florence, Italy
12MLL Munich Leukemia Laboratory, Munich, Bavaria, Germany
13Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA
14Department of Hematology, Cellular Therapy, Hemostaseology and Infectious Diseases, University Leipzig Medical Center, Leipzig, Germany
15Policlinico San Matteo, Pavia, Italy
16IRCCS Humanitas Research Hospital, Rozzano, Italy
17University of Bologna, Bologna, Italy
18IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy
19Data Science and Bioinformatics Laboratory, IRCCS Institute of Neurological Sciences of Bologna, Bologna, Italy
20Department of Internal Medicine, Section of Hematology, Yale University, New Haven, CT
21Hematology Unit, ASST Fatebenefratelli Sacco, Milan, Italy
22Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, Cleveland Clinic, Cleveland, OH
23Hematology Service, ICO-Hospital Germans Trias i Pujol, Institut de Recerca contra la Leucèmia Josep Carreras (IJC), Universitat Autònoma de Barcelona, Badalona, Spain
24Department for Hematology, Cell Therapy, Hemostaseology and Infectious Diseases, University of Leipzig, Leipzig, Saxony, Germany
25Division of Hematology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
26Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan
27Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
28Department of Internal Medicine, Far-Eastern Memorial Hospital, New Taipei, Taiwan
29MDS Unit, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy
30MLL Munich Leukemia Laboratory, Munich, Germany
31Hematology and Bone Marrow Transplantation Unit, San Raffaele Scientific Institute, Milano, Italy
32UOC Ematologia e Trapianto Cellule Staminali Emopoietiche, Fondazione Policlinico Universitario A. Gemelli IRCCS Roma, Rome, Italy
33Hematology Division and Bone Marrow Unit, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy
34Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy
353rd Medical Department with Hematology, Medical Oncology, Hemostaseology, Rheumatology and Infectiology, Oncologic Center, Salzburg, Austria
36Department of Hematology, Vall d’Hebron University Hospital, Barcelona, Spain
37Institut Català d’Oncologia, Badalona, Spain
38Cleveland Clinic Taussig Cancer Institute, Cleveland, OH
39Hospital Clínico San Cecilio, Granada, Spain
40Cooperative Group for the Study and Treatment of Acute and Myelodysplastic Leukemias (CETLAM), Barcelona, Spain
41Hospital Universitario de Salamanca, Instituto de Investigacion Biomedica de Salamanca (IBSAL), Centro de Investigación del Cancer (IBMCC-USAL, CSIC), Salamanca, Spain
42Hospital Universitario Cruces, Bilbao, Spain
43Hospital Negrin, LAS PALMAS, Spain
44Hematology Department, Consorcio Sanitario de Terrassa, Terrassa, Spain
45Hematology Department, Hospital Clínico de Valencia. Instituto de Investigación Sanitaria INCLIVA de Valencia, Valencia, Spain., Valencia, Spain
46Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX
47Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX
48Department for Hematology, Cell Therapy, Hemostaseology and Infectious Diseases, University of Leipzig Medical Center, Leipzig, Germany
49Myelodysplastic Syndromes Research Group, Institut De Recerca Josep Carreras, Badalona, Barcelona, Spain
50Hospital Clínico Universitario de Salamanca, Salamanca, Spain
51Department of Stem Cell Transplantation, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
52Hôpital Saint-Louis, Université de Paris 7, Paris, France
53Laboratory of Hematology, Université Paris Cité and Assistance Publique-Hôpitaux de Paris Centre, Hôpital Cochin, Groupe Francophone des Myelodysplasies, Paris, France
54MDS Unit, Hematology, AOUC, University of Florence, Florence, Italy
55Department of Hematology, Oncology and Clinical Immunology, University Medical Center Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany
56Hopital Saint Louis, APHP, Paris, France
57Dipartimento di Oncologia ed Ematologia, Università degli Studi di Milano, Policlinico di Milano, Ospedale Maggiore, Fondazione I.R.C.C.S. Ca Granda, Milano, Italy
58Inserm UMR866, Villejuif Cedex, France
59Department of Clinical and Experimental Medicine, University of Florence, Florence, Italy
60Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy
61Yale School of Medicine, Smilow Cancer Hospital at Yale New Haven, New Haven, CT
62Department of Malignant Hematology, Moffitt Cancer Center, Tampa, FL

Background. Recent advancements in genome characterization have transformed the study of myeloid neoplasms (MN). Accordingly, there is a shift from traditional classification schemes, based on morphological and clinical information, to next-generation systems (2022 WHO/ICC) which incorporate genomic features. However, current classifications do not clearly define the hierarchical importance of genomic vs morphological features in determining disease entities, leading to inconsistencies in clinical practice; moreover, they do not specifically address areas of overlap among different MN

Methods. In the TITAN study, we retrospectively studied 20,054 patients with MN in which clinical and morphological data, together with cytogenetics, mutational screening and outcome were available. We included 7104 AML, 8410 MDS, 2986 MDS/MPN and 1554 MF. We used MOSAIC, an AI-based framework to define unsupervised clusters according to homogeneous morphological and genomic features. Shapley Additive exPlanations (SHAP) was applied to investigate features of importance and their effects on cluster assignment

Results. We identified 34 distinct clusters and created a dashboard (https://titan-xkb3corsxq-ew.a.run.app/) where the most relevant features, clinical phenotypes, and outcomes are summarized. SHAP revealed that genomics often outweighs morphology in defining disease entities

Six clusters had splicing mutations as dominant features, albeit with different hematological phenotypes (mainly including MDS and MDS/MPN pts). The presence of additional high-risk genomic features (RUNX1/ASXL1 mutations, del(7q)/-7, abn(3q26.2) and complex karyotype) identified patients with poor survival within each category. A separate cluster including MN with splicing mutations and increased blasts (mainly including MDS and AML patients) was characterized by poor response to treatments and dismal outcome

MN with TP53 mutations constituted a distinct entity, regardless of clinical phenotype and blast count and had the poorest prognosis. Mutational and CNV analysis showed biallelic inactivation of TP53 in the majority of patients. Most monoallelic TP53 were assigned to a different cluster. Patients that evolved to AML often acquired biallelic inactivation, suggesting that TP53 allelic status may identify different disease phases within the same entity

Among morphological features, marrow fibrosis held significant hierarchical importance in defining clusters, driving patient assignment into three distinct groups characterized by 1) JAK/STAT mutations alone, 2) JAK/STAT mutations with high-risk molecular features, 3) wildtype JAK/STAT. The latter group included both MDS and MF patients who exhibited a higher risk of clonal evolution and shorter survival

Overall, 10 out of 34 clusters included patients from different MN subtypes as defined by ICC/WHO criteria, accounting for 38.1% of the entire population. This suggests that current classifications do not efficiently capture boundaries between different entities. Importantly, among 1482 patients with RNAseq data from BM progenitors available, the newly identified clusters exhibited distinct gene expression profiles, thus providing evidence for the biological consistency of the proposed classification

The 34 clusters were characterized by different risks of clonal progression and survival. Based on this finding and the observation that, in most cases, heterogeneous phenotypes contributed to each cluster, we developed a pan-MN prognostic score. This score is based on clinical and molecular parameters and is designed to be applicable to all patients, regardless of clinical labels. The performance of the score (c-index 0.75) was comparable to that of currently available disease-specific tools

Conclusion. The integrative analysis of genomic profiling and morphological features provides a proof of concept for a novel classification approach for MN, that may refine the overlapping areas among disease entities and more effectively identify patients with a homogeneous biological background. It could serve as a foundation for an innovative prognostic assessment of MN patients, independent of traditional clinical labels. The clinical implementation of pan-MN tools is expected to improve the effectiveness and quality of personalized diagnosis, treatment decisions and clinical trial eligibility

Disclosures: Gagelmann: J&J: Honoraria, Other: Travel support; BMS: Honoraria. Guglielmelli: AOP: Honoraria; Abbvie: Honoraria, Membership on an entity's Board of Directors or advisory committees; GSK: Honoraria, Membership on an entity's Board of Directors or advisory committees; Incyte: Honoraria, Membership on an entity's Board of Directors or advisory committees; Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees. Hunter: GSK: Consultancy, Honoraria; PharmaEssentia: Consultancy, Honoraria; Blueprint Medicines: Consultancy, Honoraria, Research Funding; Sobi​ (formerly CTI biopharma): Consultancy, Honoraria; Incyte: Consultancy, Honoraria, Research Funding; Cogent Biosciences: Research Funding; Ascentage Pharma: Research Funding; Syntrix Biosystems: Research Funding; Novartis: Research Funding; PharmaEssentia: Research Funding. Kubasch: Novartis: Honoraria, Research Funding; BMS: Honoraria; Curis: Research Funding; Janssen: Honoraria, Research Funding. Santoro: Sandoz: Speakers Bureau; Novartis: Speakers Bureau; Beigene: Speakers Bureau; Arqule: Speakers Bureau; Astrazeneca: Speakers Bureau; Celgene: Speakers Bureau; Amgen: Speakers Bureau; Abb-vie: Speakers Bureau; Roche: Speakers Bureau; Takeda: Speakers Bureau; MSD: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Bayer: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; EISAI: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Pfizer: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Gilead: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Servier: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; BMS: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Incyte: Consultancy; Sanofi: Consultancy; Lilly: Speakers Bureau. Xicoy: BMS: Honoraria. Vucinic: Gilead/Kite, Janssen, BMS Celgene, Novartis: Consultancy, Honoraria; Amgen: Honoraria, Other: Travel grant. Sallman: Abbvie: Consultancy; Agios: Consultancy; Axiom: Consultancy; Gilead: Consultancy; Celyad: Consultancy; Froghorn: Consultancy; Incyte: Consultancy; Intellisphere, LLC: Consultancy; Johnson & Johnson: Consultancy; Kite: Consultancy, Membership on an entity's Board of Directors or advisory committees; Magenta Therapeutics: Consultancy, Membership on an entity's Board of Directors or advisory committees; NextTech: Consultancy; Novartis: Consultancy, Membership on an entity's Board of Directors or advisory committees; AvenCell: Membership on an entity's Board of Directors or advisory committees; Astellas: Membership on an entity's Board of Directors or advisory committees; BlueBird Bio: Membership on an entity's Board of Directors or advisory committees; BMS: Membership on an entity's Board of Directors or advisory committees; Dark Blue Therapeutics: Membership on an entity's Board of Directors or advisory committees; Intellia: Membership on an entity's Board of Directors or advisory committees; Jasper Therapeutics: Membership on an entity's Board of Directors or advisory committees; NKARTA: Membership on an entity's Board of Directors or advisory committees; Orbital Therapeutics: Membership on an entity's Board of Directors or advisory committees; Rigel Pharmaceuticals: Membership on an entity's Board of Directors or advisory committees; Shattuck Labs: Membership on an entity's Board of Directors or advisory committees; Servier: Membership on an entity's Board of Directors or advisory committees; Syndax: Membership on an entity's Board of Directors or advisory committees; Syros: Membership on an entity's Board of Directors or advisory committees; Apera: Research Funding; Jazz: Research Funding. Voso: Astra Zeneca: Speakers Bureau; Abbvie: Speakers Bureau; Syros: Other: Advisory Board; Jazz: Other: Advisory Board, Speakers Bureau; Astellas: Speakers Bureau; Novartis: Other: Research support, Speakers Bureau; Celgene/BMS: Other: Research support, Advisory Board, Speakers Bureau. Pleyer: BMS: Honoraria; AbbVie: Honoraria; Otsuka: Honoraria. Jerez: Astrazeneca: Research Funding. Díaz-Beyá: BMS: Consultancy; Abbvie: Consultancy; Takeda: Consultancy; Novartis: Consultancy; Jazz Pharmaceuticals: Consultancy. Garcia-Manero: Novartis: Research Funding; Onconova: Research Funding; Helsinn: Other: Personal fees; Aprea: Research Funding; Astex: Research Funding; AbbVie: Research Funding; Genentech: Other: Personal fees; Astex: Other: Personal fees; Amphivena: Research Funding; H3 Biomedicine: Research Funding; Curis: Research Funding; Janssen: Research Funding; Genentech: Research Funding; Forty Seven: Research Funding; Helsinn: Research Funding; Bristol Myers Squibb: Other: Personal fees, Research Funding; Merck: Research Funding. Loghavi: Pathology Education Partners; VJ HemeOnc, College of American Pathologists, OncLive, ICCS, MD Education, NCCN, MashUp Media, NCTN, Aptitude Health: Honoraria; Guidepoint; QualWorld; Gerson Lehrman Group, AlphaSight, Arima, Qiagen, Opinion Health: Consultancy; Astellas, Amgen: Research Funding; Abbvie: Current holder of stock options in a privately-held company; Syndx, Servier, BMS: Membership on an entity's Board of Directors or advisory committees; Abbvie, Daiichi Sankyo, BluePrint Medicine, Caris Diagnostics, Recordati, Servier: Consultancy. Platzbecker: Amgen: Consultancy, Research Funding; BMS: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: Travel support, Research Funding; MDS Foundation: Membership on an entity's Board of Directors or advisory committees; Abbvie: Consultancy, Research Funding; Curis: Consultancy, Honoraria, Research Funding; Geron: Consultancy; Janssen: Consultancy, Honoraria, Research Funding; Merck: Research Funding; Novartis: Consultancy, Research Funding. Diez-Campelo: KEROS: Honoraria, Membership on an entity's Board of Directors or advisory committees; AGIOS: Consultancy, Membership on an entity's Board of Directors or advisory committees; Gilead: Other: Travel reimbursement; SYROS: Membership on an entity's Board of Directors or advisory committees; BMS/Celgene: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Advisory board fees; BLUEPRINT MEDICINES: Consultancy, Membership on an entity's Board of Directors or advisory committees; Novartis: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; HEMAVAN: Membership on an entity's Board of Directors or advisory committees; ASTEX/OTSUKA: Membership on an entity's Board of Directors or advisory committees, Other: TRAVEL TO MEETINGS; CURIS: Membership on an entity's Board of Directors or advisory committees; GSK: Consultancy, Membership on an entity's Board of Directors or advisory committees. Kröger: Novartis, BMS, Neovii, Kite/Gilead, Sanofi, Takada: Membership on an entity's Board of Directors or advisory committees; Novartis, Neovii, Kite/Gilead, Therakos, Alexion, Sanofi, Takeda: Other: Speaker honoraria; Novartis, DKMS: Research Funding; Provirex: Consultancy. Fenaux: Servier: Research Funding; Janssen: Research Funding; AbbVie: Honoraria, Research Funding; Jazz Pharmaceuticals: Honoraria, Research Funding; Novartis: Research Funding; Astex: Research Funding; Agios: Research Funding; BMS: Honoraria, Research Funding. Santini: Ascentage, AbbVie, Bristol Myers Squibb, CTI BioPharma, Geron, Gilead, Novartis, Servier, Syros Pharmaceuticals: Other: Advisory Board. Germing: Novatis: Honoraria; BMS: Research Funding; Abbvie: Research Funding; JAZZ: Research Funding; BMS: Honoraria. Robin: Neovii: Other: research support; Novartis: Other: research support; Abbvie: Other: research support; Medac: Other: research support. Passamonti: Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees. Solary: PEPKON: Research Funding; NOVARTIS: Speakers Bureau. Vannucchi: AOP: Consultancy, Speakers Bureau; Blueprint: Consultancy, Speakers Bureau; GSK: Consultancy, Speakers Bureau; Novartis: Consultancy, Speakers Bureau; BMS: Consultancy, Speakers Bureau; Incyte: Consultancy, Speakers Bureau. Komrokji: Novartis: Membership on an entity's Board of Directors or advisory committees; CTI biopharma: Membership on an entity's Board of Directors or advisory committees; Servio: Honoraria; Taiho: Membership on an entity's Board of Directors or advisory committees; Janssen: Consultancy; BMS: Honoraria, Membership on an entity's Board of Directors or advisory committees; DSI: Honoraria, Membership on an entity's Board of Directors or advisory committees; PharmaEssentia: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Rigel: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Jazz Pharmaceuticals: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Geron: Consultancy, Membership on an entity's Board of Directors or advisory committees; Celgene/BMS: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Genentech: Consultancy; Keros: Membership on an entity's Board of Directors or advisory committees; DSI: Consultancy, Membership on an entity's Board of Directors or advisory committees; Sumitomo Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees; Sobi: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; AbbVie: Consultancy, Membership on an entity's Board of Directors or advisory committees; BMS: Research Funding; Servier: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Servio: Membership on an entity's Board of Directors or advisory committees. Della Porta: Bristol Myers Squibb: Consultancy.

*signifies non-member of ASH