Type: Oral
Session: 704. Cellular Immunotherapies: Early Phase Clinical Trials and Toxicities: Emerging Targeting Approaches of Cell Therapies for Hematologic Malignancies
Hematology Disease Topics & Pathways:
Clinical trials, Lymphoid Leukemias, Research, ALL, Acute Myeloid Malignancies, AML, MDS, Adult, Elderly, Clinical Research, Chronic Myeloid Malignancies, Diseases, Treatment Considerations, Biological therapies, Immunotherapy, Lymphoid Malignancies, Young adult , Myeloid Malignancies, Miscellaneous Cellular Therapies, Transplantation (Allogeneic and Autologous)
The ALLOHA Study (TSCAN-001, NCT05473910) is a multi-center, biologically controlled, Phase 1 study evaluating TSC-100 and TSC-101 in adults with AML, ALL or MDS undergoing RIC HCT with a haploidentical donor. HLA-A*02:01-positive subjects receive either one or two infusions of TSC-100 or TSC-101 after count recovery, ~days 21 and 61 post HCT. Control subjects receive RIC HCT as per standard of care. Endpoints include dose limiting toxicities, safety, efficacy and exploratory biomarkers including chimerism and minimal residual disease (MRD).
As of a 08 Jul 2024 data cut, 27 subjects enrolled and underwent HCT: 16 in the treatment arm [TSC-101 (8), TSC-100 (8)] and 11 in the control arm. Three subjects had ALL (all TSC), 9 had MDS (4 TSC, 5 controls) and 15 had AML (9 TSC, 6 controls). Median age was 69 yrs in the TSC arm and 70 in controls. Most subjects had high-risk molecular or cytogenetic abnormalities, including 4 with mTP53 (2 TSC, 2 controls). Median follow-up was 5.8 months (TSC) vs 5.3 months (control).
No DLTs occurred following TSC-100/TSC-101 infusions. Safety was similar in the treatment and control arms with expected post-HCT adverse events. Grade 2-4 acute graft-versus-host disease (GvHD) was similar in treatment (2 G2 events) and control arms (1 G2, 1 G3). Similarly, one mild chronic GVHD event occurred in the treatment arm and one in the control arm. No cytokine release syndrome or neurotoxicity occurred after TSC-100/TSC-101 infusions and no TSC-100/TSC-101-related deaths occurred. Two non-relapse deaths occurred in the treatment arm: one prior to any TSC infusion and related to an adenoviral infection, and another 15 months post-HCT from a wound infection. Three deaths occurred in the control arm: one non-relapse (septic shock) and two relapse-related.
No relapses have occurred in the treatment arm vs three in the control. Median time to relapse is not evaluable in TSC-treated subjects, where no relapses occurred, vs 159 days in control arm subjects. Additionally, there was a higher probability of being relapse free at 1 year post HCT in treatment arm vs control arm subjects (p=0.047). A hazard ratio 0.09 for EFS (p=0.025) was observed at 1 year and was associated with a significantly shorter time to death or relapse event in the control arm (p=0.0049).
Translational analysis was performed in 18 subjects (11 TSC, 7 controls) with >60-days post HCT follow-up. Peak TSC expansion occurred 7-14 days post dosing, with persistence >360 days in all 5 subjects with >1-year data. The highest dose level (DL3) had substantially higher blood TSC levels. High-sensitivity chimerism analysis with NGS in whole blood, CD33+, or CD3+ cells showed complete donor chimerism (>99.8%) at Day 42 in all cells in all 11 TSCs subjects (100%) compared with 2 of 7 control subjects (29%). Post-HCT MRD by NGS (LOD <0.1% in myeloid, <0.01% in lymphoid cancers) was negative in all TSC subjects post-TSC infusion, and positive in 2 control subjects post HCT. Five TSC subjects reached 1-year follow-up with no relapse, no detectable MRD and complete donor chimerism in the malignant lineage, including a subject with mTP53/del5q MDS and other subjects with high-risk genetics/cytogenetics.
In summary, no DLTs were identified after TSC-100/TSC-101 infusions of with post-infusion safety generally consistent with HCT. All TSC subjects remained relapse free, MRD negative, with full donor chimerism in the malignant lineage, consistent with effective elimination of residual diseased cells post-HCT. The data support the safety and potential of TSC-100/TSC-101 to reduce relapses and increase event-free and relapse-free survival in RIC HCT patients. Enrollment continues and updated data will be presented.
Disclosures: Al Malki: Tscan: Consultancy; Stemline therapeutics: Research Funding; Tr1X: Consultancy; Incyte: Research Funding; CareDx: Consultancy; NexImmune: Consultancy, Research Funding. Popat: Bayer: Research Funding; Incyte: Research Funding; Abbvie: Research Funding; T Scan: Research Funding. Chen: Incyte: Consultancy; Ironwood Pharmaceuticals, Inc.: Consultancy; Vor: Consultancy; Garuda: Consultancy; Editas: Consultancy; Alexion: Consultancy. Jain: Bristol Myers Squibb, Incyte, Abbvie, CTI, Kite, Cogent Biosciences, Blueprint Medicine, Telios pharma, Protagonist therapeutics, Galapagos, Tscan therapeutics, Karyopharm, Morphosys: Membership on an entity's Board of Directors or advisory committees; CTI Biopharma, Kartos therapeutics, Incyte, Bristol Myers Squibb: Research Funding. Solh: GlaxoSmithKline: Speakers Bureau; Bristol Myers Squibb: Consultancy, Speakers Bureau; Sanofi: Consultancy. Gill: Novartis: Patents & Royalties, Research Funding; Carisma: Current holder of stock options in a privately-held company; Interius: Current holder of stock options in a privately-held company, Research Funding; Asher Biotherapeutics: Research Funding; Mission Bio: Membership on an entity's Board of Directors or advisory committees. Buonomo: TScan Therapeutics: Current Employment. Wang: TScan Therapeutics: Current Employment. Nabilsi: TScan Therapeutics: Current Employment, Current equity holder in publicly-traded company. White: TScan Therapeutics: Current Employment. Nguyen: TScan Therapeutics: Consultancy. Murray: TScan Therapeutics: Current Employment, Current equity holder in publicly-traded company. MacBeath: TScan Therapeutics: Current Employment, Current equity holder in publicly-traded company. Louis: TScan Therapeutics: Current Employment, Current equity holder in publicly-traded company; Zentalis Pharmaceuticals, CRISPR Therapeutics: Ended employment in the past 24 months. Chattopadhyay: TScan Therapeutics: Current Employment, Current equity holder in publicly-traded company. Matzko: TScan Therapeutics: Current Employment; Allovir: Divested equity in a private or publicly-traded company in the past 24 months, Ended employment in the past 24 months. Reshef: Atara Biotherapeutics: Research Funding; Immatics: Research Funding; Sanofi: Research Funding; CareDx: Research Funding; Bayer: Consultancy; BMS: Research Funding; Incyte: Consultancy, Research Funding; Takeda: Research Funding; TCR2: Research Funding; TScan: Consultancy, Research Funding; Orca Bio: Consultancy; Quell Biotherapeutics: Consultancy; Abbvie: Research Funding; Sana Biotechnology: Consultancy; Autolus: Consultancy; Cabaletta: Research Funding; Synthekine: Research Funding; J&J: Research Funding; Genentech: Research Funding; Gilead Sciences: Consultancy, Research Funding; Allogene: Consultancy; Precision Biosciences: Research Funding.
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