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3441 L-Arginine Reinforces NK Cell-Mediated GVL Effect in Bone Marrow of AML Patients with Early Relapse Post-Allo-HSCT

Program: Oral and Poster Abstracts
Session: 703. Cellular Immunotherapies other than CAR-T Cells: Basic and Translational: Poster II
Hematology Disease Topics & Pathways:
Research, Fundamental Science
Sunday, December 8, 2024, 6:00 PM-8:00 PM

Dongyao Wang, PhD1,2,3*, Chuang Yuan4*, Yue Wu5*, Xingchi Chen5*, Guangyu Sun6*, Aijie Huang, MD7*, Xiaoyu Zhu8,9 and Haiming Wei, MD10*

1Department of Hematology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei City, Anhui Province, China
2Center for Cancer Immunology and Cutaneous Biology Research Center, Department of Dermatology and Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, MA
3Institute of Immunology, School of Basic Medicine and Medical Center, University of Science and Technology of China, Hefei, China
4Peking University, Beijing, China
5Department of Hematology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230027, China, Hefei, China
6Blood and Cell Therapy Institute, Division of Life Sciences and Medicine, University of Science and Technology of China, Heifei, China
7The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Shanghai, China
8Department of Hematology, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China
9Blood and Cell Therapy Institute, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
10Institute of Immunology, School of Basic Medicine and Medical Center, University of Science and Technology of China, Hefei, Anhui, 230001, China., Hefei, CHN

Relapse is a leading cause of death after allogeneic hematopoietic stem cell transplantation (allo-HSCT) for acute myeloid leukemia (AML). However, the underlying mechanisms remain poorly understood. Natural killer (NK) cells play a crucial role in tumor surveillance and cancer immunotherapy, and NK cell dysfunction has been observed in various tumors. Here, we performed ex vivo experiments to systematically characterize the mechanisms underlying the dysfunction of bone marrow-derived NK (BMNK) cells isolated from AML patients experiencing early relapse after allo-HSCT. Using high-resolution mass spectrometry, we demonstrated that the level of L-arginine decreased in the bone marrow and was associated with BMNK cell paucity and dysfunction in these AML patients. In addition, highly expressed arginase-1 in M2 macrophages induced L-arginine deficiency in the bone marrow microenvironment and suppressed mitochondrial oxidative phosphorylation and cytotoxicity of BMNK cells. Additionally, L-arginine deficiency triggered the formation of TFEB-containing transcriptional condensates, and induced liquid-liquid phase separation of TFEB, which resulted in abnormal autophagy and apoptosis of BMNK cells. Furthermore, the supplementation with L-arginine restored NK cell-mediated anti-leukemic responses in the leukemia xenograft mouse model and patient-derived xenografted (PDX) model. Thus, our study reveals a mechanism through which L-arginine deficiency compromises NK cells, highlighting the potential of L-arginine supplementation to improve NK cell-based cancer treatments.

Disclosures: No relevant conflicts of interest to declare.

*signifies non-member of ASH