Type: Oral
Session: 604. Molecular Pharmacology and Drug Resistance: Myeloid Neoplasms: Novel Approaches to Targeting Epigenetics, Apoptosis and Immune Effectors in Myeloid Malignancies
Hematology Disease Topics & Pathways:
Research, Acute Myeloid Malignancies, AML, Combination therapy, Translational Research, Bioinformatics, Diseases, Treatment Considerations, Immunology, Myeloid Malignancies, Biological Processes, Technology and Procedures
First, we optimized the screening conditions for VEN/AZA pre-treatment, selecting the dose of 10nM VEN with 1μM AZA. This dose induced 32.7±6.8% phagocytosis (calculated as the percentage of Calcein+CD206+ cells from total CD206+ cells) of MOLM-13 cells after 24-hour pre-treatment followed by 1-hour co-culture with macrophages, compared to 24.5±6.0% in DMSO-treated cells (p=0.03). CRISPR screening using the Brunello sgRNA library revealed complementary characteristics in cells treated with VEN/AZA and VEN/AZA/macrophages. Genes including TP53, POU4F2, AKT2, ADIPOR1, etc. were positively selected in VEN/AZA-treated cells (compared to DMSO), but negatively selected in VEN/AZA/macrophages-treated cells (compared to VEN/AZA). This indicated that silencing of these genes confers leukemia cells resistance to VEN/AZA but increased sensitivity to macrophage-mediated phagocytosis. These overlapping genes were enriched in pathway regulating TP53 activity through association with co-factors (such as TP53, POU4F2 and AKT2), which influence p53 stability and the transcription of cell cycle arrest and death genes. Collectively, these data suggest that the activity of VEN/AZA in the presence of macrophages modulates TP53-dependent pathways.
To further test the hypothesis that TP53-mutant AML might derive benefit from triple therapy, we utilized TP53-mutant MOLM13 cell line and TP53-mutant patient-derived xenograft (PDX) models. In MOLM-13 cell line model, 5F9/VEN/AZA induced 40.8±2.9% phagocytosis in TP53-mutant cells and 40.5±3.1% in TP53-wild type cells (p=0.85). In the TP53-mutant PDX MDAM19692 (FLT3-ITD, DNMT3A, NPM1, TP53), mice were randomized into four groups (6-7 mice per cohort) after confirming peripheral blood engraftment by flow cytometry (14 days post-transplant). After two weeks treatment, the 5F9 and 5F9/VEN/AZA demonstrated significantly reduced leukemia burden, with an average hCD45% level of 0.3% for the 5F9 and 0.1% for combination, compared to 33.4% for vehicle, 30.0% for the VEN/AZA. The combination cohort achieved significantly better overall survival (p=0.01) with a median overall survival of 82.5 days, compared to 32, 29, and 37 days for vehicle, VEN/AZA, and 5F9 groups, respectively.
To understand the mechanisms underlying VEN/AZA-induced phagocytosis, we employed the subcellular protein fractionation and enzymatic shaving techniques to isolate different cellular compartments (including cytoplasmic, nuclear, membrane, and cytoskeletal proteins) and the exposed portions of membrane proteins at various time points during VEN/AZA treatment, respectively. Mass spectrometry analysis of the resulting peptides, followed by clustering trend analysis, based on the fuzzy C-mean algorithm, and Gene Ontology analysis of membrane proteins, revealed that VEN/AZA pretreatment primarily increased the expression of proteins involved in cellular transport and localization pathways. The shaving assay demonstrated that many of these upregulated proteins originated from the nucleus.
In summary, our study uncovered the complementary signature as a putative mechanism for the combinatorial activity of VEN/AZA therapy of AML in contact with macrophages. Our data indicate that VEN/AZA activates the translocation of nuclear proteins to the cell surface. These translocated proteins may function as damage-associated molecular patterns (DAMPs), activating macrophage pattern recognition receptors and enhancing phagocytosis. These findings provide a rationale for combining VEN/AZA with macrophage-based therapies in AML treatment, particularly in TP53-mutant cases. Further validation of the identified pathways is ongoing and will be reported.
Disclosures: Abbas: Enzyme By Design: Research Funding; Illumina: Honoraria, Other: Inkind Support, Research Funding; Molecular Partners: Consultancy; Alamar Biosciences: Honoraria; Genentech: Research Funding; GlaxoSmithKline: Research Funding; Blueprint Medicines Corporation: Research Funding; Ascentage: Research Funding. Daver: Bristol Myers Squibb: Consultancy, Research Funding; Novartis: Consultancy; Arog: Consultancy; Menarini Group: Consultancy; Servier: Consultancy, Research Funding; KITE: Research Funding; Pfizer: Consultancy, Research Funding; Gilead: Consultancy, Research Funding; Celgene: Consultancy; Jazz: Consultancy; Trillium: Consultancy, Research Funding; Astellas: Consultancy, Research Funding; Genentech: Consultancy, Research Funding; Agios: Consultancy; Shattuck Labs: Consultancy; Syndax: Consultancy; Daiichi-Sankyo: Consultancy, Research Funding; Hanmi: Research Funding; Trovagene: Research Funding; FATE Therapeutics: Other: Consulting Fees, Research Funding; Novimmune: Research Funding; Glycomimetics: Research Funding. Konopleva: Syndax: Membership on an entity's Board of Directors or advisory committees; Servier: Speakers Bureau; Sanofi Aventis: Consultancy; Janssen: Consultancy, Other: clinical trials; Dark Blue Therapeutics: Membership on an entity's Board of Directors or advisory committees; Novartis: Membership on an entity's Board of Directors or advisory committees; Intellisphere: Speakers Bureau; AbbVie: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: clinical trials, Research Funding; MEI Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees; Curis: Consultancy; Legend Biotech: Membership on an entity's Board of Directors or advisory committees; Klondike Biopharma: Research Funding; Vincerx: Consultancy; Auxenion GmbH: Membership on an entity's Board of Directors or advisory committees; Adaptive: Consultancy; Menarini Group: Consultancy, Membership on an entity's Board of Directors or advisory committees.