Session: 653. Multiple Myeloma: Clinical and Epidemiological: Poster III
Hematology Disease Topics & Pathways:
Research, Fundamental Science, Adult, Translational Research, Bioinformatics, Immunology, Biological Processes, Technology and Procedures, Study Population, Human, Omics technologies
Methods: Most current studies rely on BM aspirates, which often get contaminated by peripheral blood and lose mature granulocytes during processing. To address this limitation, we evaluated the BM cell content using a whole BM gene expression-based computational technique, CIBERSORT, and investigated the correlation of immune cell proportions with patient outcome after 5-20 years of follow-up. We also analyzed BM immune cell functional marker expression using mass cytometry in a large cohort of patients and examined BM immune cell gene expression in paired stable or progressive MM precursor conditions and MM samples using single-cell RNA sequencing (sc-RNA seq).
Results: By integrating results from CIBERSORT, mass cytometry, and sc-RNA seq, we demonstrate an altered balance of innate and adaptive immunity persisting from MGUS to MM. Specifically, the proportion of neutrophils decreasing in the tumor microenvironment (TME), predicts SMM progression and MM outcomes. HLA-DR is reduced in CD16+ monocytes and plasmacytoid dendritic cells. Myeloid dendritic cells are also decreased, and their expression of stress and immune-response genes is inhibited in MM and its precursor stages. We find that NK cells and CD8 T cells show common features with a shift from GZMK+ to GZMB+ cytotoxic phenotype in the TME compared to normal BM and this is accompanied by increased expressions of inhibitory markers TIM3 and TIGIT. Finally, we show that patient-specific GZMB+ CD8 T cell subtypes demonstrate limited changes in paired precursor and MM samples despite the increased numbers and heterogeneity of MM subclones in overt MM.
Conclusion: Overall, these results provide a comprehensive immune landscape of MM and its precursor stages. Enhancing neutrophil and NK cell cytotoxicity, tumor antigen presentation, and CD8 T cell versatility in precursor stages may prevent MM progression.
Disclosures: Alapat: EUSA Pharma: Consultancy. Al Hadidi: Janssen: Consultancy; Pfizer: Consultancy; Sanofi: Consultancy. Schinke: OncLive: Honoraria; Cancer Network: Honoraria; Pfizer: Consultancy, Honoraria, Speakers Bureau; Arcellx: Consultancy; Janssen: Consultancy, Honoraria, Speakers Bureau. Zangari: Janssen Pharmaceuticals, Inc: Consultancy, Membership on an entity's Board of Directors or advisory committees; Karyopharm: Consultancy, Membership on an entity's Board of Directors or advisory committees. van Rhee: Secura Bio: Membership on an entity's Board of Directors or advisory committees; Takeda: Consultancy; Kite Pharma: Membership on an entity's Board of Directors or advisory committees; GlaxoSmithKline: Consultancy, Membership on an entity's Board of Directors or advisory committees; EUSA Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees; Janssen Pharmaceuticals: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Castleman Disease Collaborative Network: Membership on an entity's Board of Directors or advisory committees; Karyopharm: Consultancy, Membership on an entity's Board of Directors or advisory committees; Bristol Myers Squibb: Research Funding; Adicet Bio: Membership on an entity's Board of Directors or advisory committees.
See more of: Oral and Poster Abstracts