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Description:
This scientific session will encompass an overview of novel technologies, applications to understanding clonal heterogeneity in tissues, spatial and temporal regulatory mechanism that shape cellular identity and heterogeneity, and high-resolution mapping of chromatin in situ.
Dr. Fei Chen will discuss a toolbox for single-cell spatial genomics. He will provide an overview of recent advances in the technology of single cell sequencing in the context of tissues. Specifically, he will describe advances in situ and single cell transcriptomic sequencing tools which aim to capture both the spatial context of cells within tissues and their gene expression programs. Dr. Chen will provide an overview of a novel approach for single-cell spatial genomics, as well as applications in immune receptor profiling, cell-cell interactions in the tumor microenvironment, and in situ genetic alterations.
Dr. Elham Azizi will discuss spatial and temporal mechanisms that underlie the leukemic microenvironment. Using longitudinal single cell transcriptomic and spatial protein imaging data from patients with relapsed acute myeloid leukemia and chronic myeloid leukemia following hematopoietic Stem Cell Transplantation., Dr. Azizi has designed and implemented computational frameworks for integration and interpretation of this data and a novel machine learning tool for time-resolved prediction of cell-cell interactions. In doing so, Dr. Azizi’s work has revealed disease-specific coordinated immune networks associated with the inhibition of leukemia cells in responder patients, which can be leveraged in the development of next-generation therapeutic strategies.
Dr. Rong Fan will discuss novel methods and technologies for profiling of proteins, transcriptomes, and chromatin at the single-cell level in tissues. While single-cell methods in dissociated tissue samples provide remarkable insights into the identity and function of cells and have been successful in identifying new cell types, spatial multi-omics are predicted to provide novel insights. Dr. Fan will discuss approaches to profile proteins, whole transcriptomes, chromatin accessibility and regulation in situ. He will also highlight potential applications to understanding spatially resolved cellular function in hematopoietic and lymphoid tissues.
Dr. Fei Chen will discuss a toolbox for single-cell spatial genomics. He will provide an overview of recent advances in the technology of single cell sequencing in the context of tissues. Specifically, he will describe advances in situ and single cell transcriptomic sequencing tools which aim to capture both the spatial context of cells within tissues and their gene expression programs. Dr. Chen will provide an overview of a novel approach for single-cell spatial genomics, as well as applications in immune receptor profiling, cell-cell interactions in the tumor microenvironment, and in situ genetic alterations.
Dr. Elham Azizi will discuss spatial and temporal mechanisms that underlie the leukemic microenvironment. Using longitudinal single cell transcriptomic and spatial protein imaging data from patients with relapsed acute myeloid leukemia and chronic myeloid leukemia following hematopoietic Stem Cell Transplantation., Dr. Azizi has designed and implemented computational frameworks for integration and interpretation of this data and a novel machine learning tool for time-resolved prediction of cell-cell interactions. In doing so, Dr. Azizi’s work has revealed disease-specific coordinated immune networks associated with the inhibition of leukemia cells in responder patients, which can be leveraged in the development of next-generation therapeutic strategies.
Dr. Rong Fan will discuss novel methods and technologies for profiling of proteins, transcriptomes, and chromatin at the single-cell level in tissues. While single-cell methods in dissociated tissue samples provide remarkable insights into the identity and function of cells and have been successful in identifying new cell types, spatial multi-omics are predicted to provide novel insights. Dr. Fan will discuss approaches to profile proteins, whole transcriptomes, chromatin accessibility and regulation in situ. He will also highlight potential applications to understanding spatially resolved cellular function in hematopoietic and lymphoid tissues.