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4048 Vitamin C Limits Multipotent Progenitor Self-Renewal and Clonal Expansion

Program: Oral and Poster Abstracts
Session: 501. Hematopoietic Stem and Progenitor Cells and Hematopoiesis: Basic and Translational: Poster III
Hematology Disease Topics & Pathways:
Fundamental Science, Research, hematopoiesis, metabolism, Biological Processes
Monday, December 11, 2023, 6:00 PM-8:00 PM

Stefano Comazzetto, PhD1*, Daniel Cassidy1*, Bethany Davis1*, Amanda Reyes1* and Sean Morrison, PhD2

1Children's Research Institute, UT Southwestern Medical Center, Dallas, TX
2University of Texas Southwestern Medical Center, Dallas, TX

A fundamental question is whether physiological variations in diet-derived metabolite levels in vivo influence stem and progenitor cell self-renewal. Ascorbate (vitamin C) is enriched in hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) compared to all other hematopoietic cells, and ascorbate depletion increases HSC function by reducing Tet2 function (Agathocleous et al., 2017). However, whether ascorbate regulates the self-renewal of other hematopoietic progenitors downstream of HSCs is still unknown.

To answer this question, we conditionally deleted Slc23a2, the gene that encodes the main hematopoietic ascorbate transporter, in murine hematopoietic cells using Mx1-Cre. Deletion of Slc23a2 substantially increased the reconstituting potential of bone marrow cells upon competitive transplantation into irradiated mice. Slc23a2 deletion only mildly increased donor chimerism in HSCs (Lin- Kit+ Sca1+ CD150+ CD48-) in the reconstituted mice, but significantly increased donor chimerism in MPPs (Lin- Kit+ Sca1+ CD150- CD48-) and downstream progenitors, suggesting that ascorbate depletion enhanced MPP self-renewal. Deletion of the ascorbate transporter also increased the long-term reconstituting potential of purified MPPs in irradiated mice, but not the long-term reconstituting potential of purified HPC1 (Lin- Kit+ Sca1+ CD150- CD48+) or HPC2 (Lin- Kit+ Sca1+ CD150+ CD48+) progenitor cells. These data showed that ascorbate depletion conferred long-term reconstituting potential upon MPPs.

To understand how ascorbate depletion enhanced MPP self-renewal, we measured the composition and proliferation rate of MPPs. Slc23a2 deletion significantly reduced MPP proliferation, and it increased the frequency of the more quiescent MPP1 (CD229- CD244-) subpopulation while decreasing the frequency of less quiescent MPP2 (CD229+ CD244-) and MPP3 (CD229+ CD244+) subpopulations (Oguro et al., 2013). Single-cell RNA-Seq (scRNA-Seq) analysis revealed that Slc23a2 deletion increased the frequency of MPPs with high expression of self-renewal gene signatures (Rodriguez-Fraticelli et al., 2020). Taken together, these data suggested that ascorbate depletion promoted MPP self-renewal through the expansion of a subpopulation of quiescent MPPs. To test this, we evaluated the division history of wild-type and Slc23a2-deficient MPPs using H2B-GFP reporter mice (Foudi et al., 2009). Slc23a2 deletion significantly increased the percentage of H2B-GFPHigh quiescent MPPs, while reducing the percentage of H2B-GFPNeg proliferating MPPs as compared to controls. Competitive transplantation of purified H2B-GFPHigh or H2B-GFPNeg MPPs revealed that Slc23a2 deletion increased the reconstituting potential of H2B-GFPHigh quiescent MPPs as compared to controls, but not the reconstituting potential of H2B-GFPNeg proliferating MPPs. Our data thus showed that ascorbate depletion enhanced MPP long-term self-renewal by expanding a subpopulation of quiescent MPPs.

Overall, we showed that ascorbate cell-autonomously limited MPP self-renewal potential by negatively regulating MPP quiescence. Our study thus points to a central role for diet-derived nutrients in the regulation of HSC and hematopoietic progenitor self-renewal abilities. Additionally, our data suggest that nutrition might directly impact the expansion of mutated cells found in clonal hematopoiesis.

Disclosures: No relevant conflicts of interest to declare.

*signifies non-member of ASH