Session: 605. Molecular Pharmacology and Drug Resistance: Lymphoid Neoplasms: Poster II
Hematology Disease Topics & Pathways:
Research, Biological therapies, non-Hodgkin lymphoma, Translational Research, Lymphomas, Bispecific Antibody Therapy, Diseases, Therapies, Lymphoid Malignancies
NHL cell lines, SU-DHL-16 and MAVER-1, were engineered using CRISPR-Cas9 system to create CD20 knock out (KO) cell lines. Wild-type (WT) or mutant CD20 were exogenously re-expressed in the CD20 KO cell lines and in the CD20 negative acute lymphoblastic leukemia cell line, REH, to create isogenic cell lines for further analysis. Complete CD20 loss was observed in cells expressing CD20 P160fs (frameshift) and Q187* (truncating) mutations. CD20 missense mutations located in the extracellular domain (C167G, K175E) expressed CD20 protein levels comparable to CD20 WT cells and membrane localization was confirmed by flow cytometry and immunofluorescence microscopy. To test the functional effect of CD20 mutations, in vitro co-culture cell killing assays were carried out using healthy donor CD8+ T cells, isogenic cell lines (3:1 effector to tumor cell ratio) and a proof-of-concept CD20-CD3 bispecific antibody. Differences in T-cell mediated killing were observed across mutation types. CD20 WT cell lines demonstrated 95-99% cell killing following 48 hour treatment while the CD20 KO, frameshift and truncating mutations all showed negligible T-cell mediated killing (~10-17% cell death). Cells expressing the missense mutations in the extracellular domain showed resistance to T-cell mediated killing (~30-45% cell death) compared to CD20 WT cells. T-cell activation was assessed by CD69 (early) and CD25 (late) surface markers. In all co-cultures, the level of CD69+ T cells was equivalent, while the CD69+CD25+ CD8 T cells were only significantly increased in the CD20 WT co-cultures, consistent with the high level of T-cell mediating killing.
We have confirmed that these mutations can play a role in the development of resistance through both loss of target protein expression and interference within the anti-CD20 binding site. These studies help to characterize mechanisms of resistance to mosunetuzumab that could be extended to other anti-CD20 targeted agents.
[1] Schuster et al. Journal of Clinical Oncology 2022, 40 (16), 7526.
Disclosures: Maximov: Genentech, Inc.: Current Employment; F Hoffmann-La Roche Ltd: Current equity holder in publicly-traded company. Bolen: Genentech, Inc.: Current Employment; F Hoffmann-La Roche Ltd: Current equity holder in publicly-traded company. Polson: F Hoffmann-La Roche Ltd: Current equity holder in publicly-traded company; Genentech: Current Employment. Penuel: Genentech, Inc. / F. Hoffman-La Roche Ltd: Current Employment, Current holder of stock options in a privately-held company. Lasater: Genentech: Current Employment; F Hoffmann-La Roche Ltd: Current equity holder in publicly-traded company.
See more of: Oral and Poster Abstracts