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3946 Natural IgM Recognizing Oxidation-Specific Epitopes Attenuate NETosis Induced By Extracellular Vesicles

Program: Oral and Poster Abstracts
Session: 301. Vasculature, Endothelium, Thrombosis and Platelets: Basic and Translational: Poster III
Hematology Disease Topics & Pathways:
Research, Fundamental Science, Bleeding and Clotting, thromboembolism, Diseases, thrombotic disorders, immunology, Adverse Events, Biological Processes
Monday, December 11, 2023, 6:00 PM-8:00 PM

Anna Ondracek1*, Taras Afonyushkin, PhD2*, Adrienne Aszlan1*, Soreen Taqi3*, Thomas Koller, MD2*, Tyler Artner4*, Florentina Porsch, PhD2*, Smriti Sharma4*, Thomas Sherz5*, Thomas M Hofbauer1*, Maria Ozsvar Kozma2*, Veronika Seidl6*, Dietrich Beitzke7*, Christoph Testori8*, Irene Lang4* and Christoph J Binder2*

1Department of Internal Medicine II, Cardiology, Medical University of Vienna, Vienna, Austria
2Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria
3Department of Laboratory Medicine, Medical University of Vienna, Vienna, AUT
4Department of Internal Medicine II, Cardiology, Medical University of Vienna, Vienna, AUT
5Department of Dermatology, Landesklinikum Wiener Neustadt, Wiener Neustadt, Austria
6Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria
7Department of Emergency Medicine,, Medical University of Vienna, Vienna, Austria
8Medical University of Vienna, Vienna, Austria


Neutrophil extracellular traps (NETs) emerged as important contributors to thrombus formation. We have previously demonstrated the role of NETs in acute myocardial infarction (AMI). However, the mechanistic understanding of modulators of NETosis in the context of AMI is scarce. Levels of extracellular vesicles (EV) carrying lipid peroxidation-derived epitopes also called oxidation-specific epitopes (OSE), are increased at the culprit site in AMI. Importantly, natural IgM antibodies with specificity for OSE have been shown to inhibit the pro-coagulatory and pro-inflammatory effects of EV, and low plasma levels predict cardiovascular risk. We hypothesized that large EV, also called microvesicles (MV), induce NETosis during AMI, and that natural anti-OSE IgM are inhibiting this process.


Patients were consented when the decision for primary percutaneous intervention (pPCI) for acute ST-segment elevation myocardial infarction (STEMI) was made, and blood was aspirated from the culprit site and peripheral site as an in-patient systemic control (n=28). Myocardial function was assessed by cardiac magnetic resonance imaging (cMRI) 4±2 days and 195±15 days after pPCI. EV were isolated from patient culprit site plasma and cell culture supernatants and characterized by flow cytometry. Isolated EV were used for neutrophil stimulation in vitro, and in vivo using a murine injection model in the presence of the IgM LR04 recognizing the immunodominant OSE, malondialdehyde (MDA) epitopes, or an isotype control. NETs were visualized by immunofluorescence staining for DNA-histone, citrullinated histone 3 (citH3) and myeloperoxidase (MPO). NET markers and natural IgM recognizing OSE in murine and patient plasma were measured by ELISA.


Culprit site plasma contained more and proportionally higher levels of leukocyte-derived CD45+ MDA-EV compared to the peripheral site plasma. NET markers were associated with CD45+ MDA-EV at the site of occlusion. Decreased levels of MDA-specific IgM at the culprit site indicated consumption of protective IgM. EV isolated from AMI patient plasma induced NETosis in vitro and after injection into C57BL/6 mice in vivo, as determined by NET markers and fluorescence microscopy of histone citrullination in neutrophils. The malondialdehyde-specific IgM LR04, but not a control IgM, reduced the NETogenic effects of EV in vitro and in vivo. Higher circulating levels of EV and lower OSE-IgM were associated with reduced ejection fraction in AMI patients at follow-up.


EV from AMI patients induced NET formation in vitro and in vivo, and natural IgM recognizing malondialdehyde-epitopes attenuated this effect. In summary, the balance between OSE-EV and OSE-IgM at the culprit site during AMI may represent a potential prognostic and therapeutic target. Moreover, the modulatory effect of natural IgM may also apply to other pathologies where NETosis contributes to thrombus formation.

Disclosures: No relevant conflicts of interest to declare.

*signifies non-member of ASH