64th ASH Annual Meeting and Exposition: 506. Bone Marrow Microenvironment: Extrinsic Regulators of Hematopoiesis

Symposia: Bone Marrow Microenvironment Oral and Poster Abstracts
Oral

Research, Fundamental Science, Acute Myeloid Malignancies, AML, Translational Research, assays, MPN, bioinformatics, hematopoiesis, Chronic Myeloid Malignancies, Diseases, Myeloid Malignancies, Biological Processes, emerging technologies, Technology and Procedures, multi-systemic interactions, imaging, omics technologies

260-262 (Ernest N. Morial Convention Center)

Moderators:

Marta Derecka, PhD, St Jude Children's Research Hospital and Nicki Panoskaltsis, MD, PhD, Trinity College Dublin

Disclosures:

Panoskaltsis: SANICKA, Inc: Current equity holder in private company; Emory University: Patents & Royalties; Beacon Targeted Therapies: Other: Key Opinion Leader.

This session investigates how hematopoiesis is extrinsically regulated. The session includes endothelial cells, stromal cells and modeling of organoids.

10:30 AM

697

Endothelial mTOR Preserves Hematopoietic Stem Cell Fitness By Suppressing Thrombospondin1

Pradeep Ramalingam, MD, PhD, Jason Butler, PhD^* and Michael Poulos, PhD

Department of Hematology/Oncology, University of Florida, Gainesville, FL

10:45 AM

698

Human Bone Marrow Organoids Enable the Study of Hematopoietic Cell-Stromal Interactions and Support the Survival of Malignant Cells from Patients

Abdullah Khan, PhD^1,2, Antonio Rodriguez-Romera²^*, Michela Colombo²^*, Jasmeet S Reyat³^*, Guanlin Wang, PhD^4,5^*, Wei Xiong Wen^1,6^*, Lauren C Murphy²^*, Rebecca Ling²^*, Natalina Elliott, PhD⁷^*, Nikolaos Sousos, MD²^*, Samuel Kemble, PhD, BSc⁸^*, Beata Grygielska³^*, Christopher Mahoney⁸^*, Andrew P. Stone, PhD⁹^*, Adam P Croft⁸^*, David Bassett¹⁰^*, Gowsihan Poologasundarampillai¹¹^*, Adele K. Fielding, MBBS, PhD¹², Emily A. Cutler, MSc¹³^*, Anindita Roy, MD, PhD⁷, Sarah Gooding, MD, PhD^14,15^*, Julie Rayes, PhD³^*, Kellie Machlus, PhD¹⁶ and Bethan Psaila, MD, PhD^1,15

¹Medical Research Council Weatherall Institute of Molecular Medicine and NIHR Biomedical Research Centre, University of Oxford, Oxford, United Kingdom
²Radcliffe Department of Medicine and National Institute of Health Research (NIHR), MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, Oxford, United Kingdom
³Institute of Cardiovascular Science, University of Birmingham, Birmingham, United Kingdom
⁴MRC WIMM Centre for Computational Biology, University of Oxford, U.K, Oxford, United Kingdom
⁵Radcliffe Department of Medicine and National Institute of Health Research (NIHR), MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom
⁶MRC WIMM Centre for Computational Biology, University of Oxford, Oxford, United Kingdom
⁷Department of Paediatrics and MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom
⁸Rheumatology Research Group, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, United Kingdom
⁹Vascular Biology Program, Harvard Medical School and Boston Children's Hospital, Boston, MA
¹⁰Healthcare Technologies Institute, School of Chemical Engineering, University of Birmingham, Birmingham, United Kingdom
¹¹School of Dentistry, Institute of Clinical Sciences, University of Birmingham, Birmingham, United Kingdom
¹²UCL Cancer Institute - Department of Haematology, University College London, London, United Kingdom
¹³UCL Cancer Institute, London, United Kingdom
¹⁴Radcliffe Department of Medicine and National Institute of Health Research (NIHR), MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, Oxford, ENG, United Kingdom
¹⁵Oxford University Hospitals NHS Trust, Oxford, United Kingdom
¹⁶Boston Children's Hospital, Boston, MA

11:00 AM

699

Generation of Human Induced Pluripotent Stem Cell-Derived Bone Marrow Organoids

Stephanie Frenz, MD¹^*, Isabel Goek, MSc²^*, Maximilian Buser, PhD³^*, Kirill Salewskij, MSc⁴^*, Savannah Fairley²^*, Raffaele Conca, MSc²^*, Nicole Drexler⁵^*, Gustav Jonsson⁴^*, Moritz Thomas, MSc³^*, Yoko Mizoguchi, MD PhD⁶^*, Martina Rudelius, MD⁷^*, Thomas Heuser, PhD⁵^*, Carsten Marr, PhD³^*, Josef M Penninger, MD⁴^* and Christoph Klein, MD PhD²

¹Department of Pediatrics, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-Universität München, München, Germany
²Department of Pediatrics, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-Universität München, Munich, Germany
³Institute of AI for Health, Helmholtz Munich – German Research Center for Environmental Health, Neuherberg, Germany
⁴Institute of Molecular Biotechnology Austria (IMBA), Vienna, Austria
⁵Electron Microscopy Facility at Vienna BioCenter Core Facilities GmbH (VBCF), Vienna, Austria
⁶Department of Pediatrics, Hiroshima University Hospital, Hiroshima, Japan
⁷Institute of Pathology, Faculty of Medicine, Ludwig-Maximilians-Universität München, Munich, Germany

11:15 AM

700

Haemogenic Gastruloids Recapitulate Developmental Haematopoiesis and Provide an Ontogeny-Relevant Context to Dissect the Origins of Infant Leukemia

Denise Ragusa^1,2^*, Chun-Wai Suen³^*, Gabriel Torregrosa⁴^*, Liza Dijkhuis²^*, Connor Byrne²^*, Tina Balayo⁴^*, Andrea Tavosanis⁵^*, Jozef Durko⁵^*, Kristen Place³^*, Ana Filipa Domingues⁶^*, Kamil Kranc⁵^*, Jordi Garcia-Ojalvo⁴^*, Alfonso Martinez Arias⁴^* and Cristina Pina, MD^1,2^*

¹Genome Engineering and Maintenance Network (CenGEM), Institute of Environment, Health and Societies, Brunel University London, Uxbridge, United Kingdom
²College of Health, Medicine and Life Sciences, Division of Biosciences, Brunel University London, Uxbridge, United Kingdom
³Department of Genetics, University of Cambridge, Cambridge, United Kingdom
⁴Universidad Pompeu Fabra, Barcelona, Spain
⁵Barts Cancer Institute, Queen Mary University of London, London, United Kingdom
⁶Department of Haematology, University of Cambridge, Cambridge, United Kingdom

11:30 AM

701

First Documentation of Cell-to-Cell Peroxisome Transfer in Hematopoietic Stem and Progenitor Cells: A Method to “Relieve Stress”?

Wilaiwan Durose¹^*, Ashish O. Gupta, MBBS, MPH², Paul J. Orchard, MD²^*, Erin Nolan¹^*, Ashley C. Kramer, BS³^* and Troy C. Lund, MD, PhD, MS²

¹University of Minnesota, Minneapolis, MN
²Division of Pediatric Blood and Marrow Transplantation, Department of Pediatrics, University of Minnesota, Minneapolis, MN
³Wayne State University - School of Medicine, Detroid, MI

11:45 AM

702

Using Spatial Transcriptomics to Reveal Fetal Liver Hematopoietic Stem Cell-Niche Interactions

Ruochen Dong, PhD, BM¹, Jonathon Russell¹^*, Hua Li¹^*, Seth Malloy¹^*, Kate Hall¹^*, Kaitlyn Petentler¹^*, Allison Scott¹^*, Cathy Mckinney²^*, Sarah E Smith¹^*, Yongfu Wang¹^*, Anoja Perera¹^*, Sean McKinney¹^*, Brian Slaughter¹^*, Jay Unruh¹^*, Xi C He¹ and Linheng Li, PhD¹

¹Stowers Institute for Medical Research, Kansas City, MO
²Stowers Insititue for Medical Reserach, Kansas City, MO

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^*signifies non-member of ASH

506.O1.6 506. Bone Marrow Microenvironment: Extrinsic Regulators of Hematopoiesis