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Hematology Disease Topics & Pathways:
Research, Clinical Practice (Health Services and Quality), Translational Research, genomics, Diseases, Immune Disorders, immune mechanism, Therapies, immunology, Lymphoid Malignancies, computational biology, Biological Processes, Myeloid Malignancies, emerging technologies, Technology and Procedures, profiling, machine learning, omics technologies
Description:
Rapid advances in Next Generation Sequencing (NGS) have led to a revolution in the genomic analysis of tumors and other pathologic conditions. During the same time there have also been innovations in spatial biology facilitating multiparameter studies that monitor complex protein expression patterns in tissue. NGS genomics data are now being directly combined with spatial analysis to enable the study of single cells and cell types within their tissue context. This session will highlight recent cutting-edge research on the development and application of spatial biology and spatial transcriptomics to tumor biology with a focus on morphology and hematopoietic neoplasms.
Dr. Rong Fan will describe the emerging technologies developed in his laboratory for spatially resolved genome-wide tissue profiling at the cellular level via deterministic barcoding in tissue (DBiT). This omics approach encompasses the spatial mapping of proteins, transcriptome, chromatin accessibility and histone modifications. He will also discuss the application of these technologies to the human lymph node, tonsil, and bone marrow tissues from both healthy donors and patients with hematological malignancies. Spatially resolved multi-omics atlases from these tissues will provide a valuable resource to the field for examining the role of cell-cell interaction and niches in health and disease, and as a potential strategy for therapeutic intervention.
Dr. Peter Sorger will describe the use of highly multiplexed tissue imaging and spatial transcriptomics to study key features of tumor-immune interaction with an emphasis on the invasive margins of solid cancers. The formation of specific immune niches in tumors will be described, as well as the relationships of these niches to the overall organization of spatial tumors. Applications of spatial profiling to bone marrow and liquid tumors will be discussed, as will the impact of highly multiplexed methods on histopathology more generally.
Dr. Simon Haas will describe the application of single-cell and spatially-resolved technologies to gain a holistic understanding of the micro- and macro-anatomy of hematological organs, such as the lymph node. His studies will focus on the spatial evolution of lymphomas and will address the long-standing question of why some lymphomas grow in an organized manner, whereas others grow diffusely- an important topic that has profound clinical implications. Dr. Haas will demonstrate that a reprogramming of the lymph node microenvironment underlies loss of compartmentalization in aggressive lymphomas, illustrating the power of combined single-cell and spatially-resolved technologies for deciphering molecular pathomechanisms.
Dr. Rong Fan will describe the emerging technologies developed in his laboratory for spatially resolved genome-wide tissue profiling at the cellular level via deterministic barcoding in tissue (DBiT). This omics approach encompasses the spatial mapping of proteins, transcriptome, chromatin accessibility and histone modifications. He will also discuss the application of these technologies to the human lymph node, tonsil, and bone marrow tissues from both healthy donors and patients with hematological malignancies. Spatially resolved multi-omics atlases from these tissues will provide a valuable resource to the field for examining the role of cell-cell interaction and niches in health and disease, and as a potential strategy for therapeutic intervention.
Dr. Peter Sorger will describe the use of highly multiplexed tissue imaging and spatial transcriptomics to study key features of tumor-immune interaction with an emphasis on the invasive margins of solid cancers. The formation of specific immune niches in tumors will be described, as well as the relationships of these niches to the overall organization of spatial tumors. Applications of spatial profiling to bone marrow and liquid tumors will be discussed, as will the impact of highly multiplexed methods on histopathology more generally.
Dr. Simon Haas will describe the application of single-cell and spatially-resolved technologies to gain a holistic understanding of the micro- and macro-anatomy of hematological organs, such as the lymph node. His studies will focus on the spatial evolution of lymphomas and will address the long-standing question of why some lymphomas grow in an organized manner, whereas others grow diffusely- an important topic that has profound clinical implications. Dr. Haas will demonstrate that a reprogramming of the lymph node microenvironment underlies loss of compartmentalization in aggressive lymphomas, illustrating the power of combined single-cell and spatially-resolved technologies for deciphering molecular pathomechanisms.