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1484 Secondary AML Mutations Confer Poor Prognosis in Patients with ELN Favorable Risk NPM1-Mutated AML

Program: Oral and Poster Abstracts
Session: 617. Acute Myeloid Leukemias: Biomarkers, Molecular Markers and Minimal Residual Disease in Diagnosis and Prognosis: Poster I
Hematology Disease Topics & Pathways:
Research, Acute Myeloid Malignancies, AML, adult, Clinical Research, Diseases, real-world evidence, Myeloid Malignancies, Study Population, Human, Minimal Residual Disease , molecular testing
Saturday, December 10, 2022, 5:30 PM-7:30 PM

Onyee Chan, MD1, Najla Al Ali, MD1*, Hammad Tashkandi, MBBS2, Austin Ellis, MD3*, Somedeb Ball, MD1, Justin Grenet, MD4, Caroline Hana, MBBCh5, Yehuda E. Deutsch, MD6, Ling Zhang, MD7*, Mohammad O Hussaini, MD8*, Jinming Song, MD, PhD9*, Seongseok Yun, MD, PhD1, Chetasi Talati, MD1*, Andrew Kuykendall, MD10, Eric Padron, MD11, Alison R. Walker, MD1, Gail J. Roboz, MD12, Pinkal Desai, MD, MPH12, David A. Sallman, MD11, Kendra Sweet, MD11, Rami S. Komrokji1 and Jeffrey E. Lancet, MD1

1Department of Malignant Hematology, Moffitt Cancer Center, Tampa, FL
2Moffitt Cancer Center, Tampa, FL
3Department of Pathology, Moffitt Cancer Center, Tampa, FL
4New York Presbyterian/Weill-Cornell Medical Center, New York, NY
5University of Miami/JFK Palm Beach Regional Campus, Boynton Beach, FL
6Department of Malignant Hematology and Cellular Therapy at Memorial Health System, Moffitt Cancer Center, Pembroke Pines, FL
7Department of Hematopathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL
8Department of Hematopathology and Lab Medicine, Moffitt Cancer Center, Tampa, FL
9Department of Hematopathology, Moffitt Cancer Center, Tampa, FL
10Department of Malignant Hematology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL
11Department of Malignant Hematology, H. Lee Moffitt Cancer Center, Tampa, FL
12Division of Hematology and Oncology, Weill Cornell Medicine and The New York-Presbyterian Hospital, New York, NY

Background: NPM1 is mutated in approximately 30% of patients with acute myeloid leukemia (AML) and is one of the mutations that defines favorable risk by European LeukemiaNet (ELN) 2017 criteria. Mutations that are highly specific for secondary AML including ASXL1, BCOR, EZH2, RUNX1, SF3B1, SRSF2, STAG2, U2AF1, and ZRSR2 (sMut) (Lindsley et al.) have been shown to confer poor prognosis. The overall impact of these mutations in the setting of NPM1-mutated AML remains unclear.

Objective: In this study, we examined the outcomes in patients with NPM1-mutated AML with other potential adverse prognostic factors including sMut and measurable residual disease (MRD).

Methods: This was a multicenter, retrospective study (Moffitt Cancer Center, Weill Cornell, and Memorial Healthcare System) of NPM1-mutated AML patients who were diagnosed and treated from 2013 to June 2022. Inclusion was restricted to patients with NPM1-mutated AML who had mutation analysis (NGS) performed at diagnosis (n=233). Kaplan-Meier, univariate (log-rank), and multivariate (Cox regression) analyses were performed.

Results: Among 233 patients (111M/122F, median age 63 (22-86) at diagnosis), 81.1% had de novo AML (dAML). By ELN 2017 criteria, 58.8% (137/233) had favorable risk, 29.2% (68/233) had intermediate risk, and 9.4% (22/233) had adverse risk disease. Over 88% had intermediate risk cytogenetics at the time of diagnosis. Common co-mutations included DNMT3A (48.9%), FLT3-ITD (33.9%), TET2 (28.7%), IDH1 (15.8%), FLT3-TKD (15%), NRAS (12.4%), and IDH2 (11.6%) (Table 1).

Forty-three (18.5%) patients had sMut, 30 (69.8%) of whom had ELN favorable risk disease (median age 67, range 46-86). Patients with sMut achieved a CR/CRi rate of 69.7% (30/43) compared to 85.3% (162/190) of patients without sMut (p=0.025). Approximately 35% of the sMut cohort proceeded to transplant compared to 50% in those without sMut (p=0.093). The median overall survival (mOS) of the entire cohort was 35.5 months with a median follow up of 52.1 months. Those who harbored sMut had worse OS compared to those without sMut (15.3 months vs 43.7 months, p=0.002). Among patients with ELN favorable-risk disease, OS was 14.7 months vs. not reached for those with sMut and without sMut, respectively (p<0.0001) (Figure 1).

For patients with ELN favorable risk, univariate analysis showed age over 60 at diagnosis, therapy-related AML (tAML), and sMut significantly impacted OS (age over 60: HR 2.67, 95% CI: 1.49-4.76, p<0.001; tAML (vs. dAML): HR 4.34, 95% CI: 1.54-12.27, p=0.006; sMut: HR 2.95, 95% CI: 1.76-4.95, p<0.001). Multivariate analysis using covariates including age over 60, AML type, sMut, and mutation burden (3 or more) confirmed the prognostic significance of sMut on OS (sMut: HR 2.12, 95% CI: 1.16-3.87, p=0.015).

Post-treatment MRD testing by qPCR for mutant NPM1 was performed in 96/233 (41.2%) patients. Patients who achieved MRD negativity predicted for longer OS compared to those with MRD positivity (MRD negative: mOS 73.9 months vs. MRD positive: mOS 34.5 months, p=0.009). Similarly, MRD negativity predicted for longer OS in the sMut subset (n=13 [9 MRD negative and 4 MRD positive], mOS 73.9 vs. 12.3 months, p=0.017). Further subgroup analysis using the favorable risk sMut cohort again showed statistical significance based on MRD status (n=8 [6 MRD negative and 2 MRD positive], mOS 27.3 vs. 10.5 months, p=0.009).

Conclusions: Our findings demonstrate that the co-occurrence sMut confers a significantly adverse effect on the survival of NPM1-mutated AML, despite being primarily classified as favorable risk by ELN 2017. These data support the recent ELN 2022 recommendations of sMut as an adverse prognostic indicator, even in the setting of otherwise favorable risk categorization. MRD negativity for NPM1 mutations was associated with improved OS, even in the presence of sMut, suggesting its importance as a prognostic factor across different risk groups.

Disclosures: Chan: Syntrix Pharmaceuticals: Research Funding. Hussaini: Adaptive Biotechnologies: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Amgen: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Aptitude Health: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Blueprint Oncology: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Celgene: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Decibio: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Diaceutics: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Guidepoint: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Seattle Genetics: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Stemline: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Tegus: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Janssen: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; BMS: Consultancy, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau. Kuykendall: Protagonist: Other: Research Support; Imago Biosciences: Consultancy, Honoraria, Speakers Bureau; Incyte: Consultancy, Honoraria, Speakers Bureau; Blueprint Medicines Corporation: Consultancy, Honoraria, Research Funding, Speakers Bureau; Novartis: Consultancy, Honoraria, Speakers Bureau; Abbvie: Consultancy, Honoraria, Speakers Bureau; GSK - Sierra Oncology: Consultancy, Honoraria, Other: Research Support, Speakers Bureau; Prelude Pharmaceuticals: Other: Research Support; BMS: Consultancy, Honoraria, Other: Research Support, Speakers Bureau; Morphosys: Other: Research Support; Pharmaessentia: Consultancy, Honoraria, Speakers Bureau; CTI Biopharma: Consultancy, Honoraria, Speakers Bureau. Padron: Syntrix Pharmaceuticals: Research Funding; Blueprint: Honoraria; BMS: Research Funding; Incyte: Research Funding; Kura: Research Funding; Stemline: Honoraria; Taiho: Honoraria. Roboz: Mofitt Cancer Center: Research Funding; Otsuka: Consultancy; Astex Pharmaceuticals: Consultancy, Other: Travel and Accommodation expenses, Research Funding; MedImmune: Consultancy, Research Funding; Bayer: Consultancy, Other: Travel and accommodation expenses; Pfizer: Consultancy, Honoraria, Other: Travel and accommodation expenses; Celltrion: Consultancy, Other: Travel and accommodation expenses; Genentech/Roche: Consultancy, Other: Travel and accommodation expenses; Sandoz: Consultancy, Other: Travel and accommodation expenses; Daiichi Sankyo: Consultancy; MEI Pharma: Consultancy, Research Funding; Bristol Myers Squibb: Consultancy; Takeda: Consultancy; Jazz: Consultancy, Other: travel; Jasper Therapeutics: Consultancy; Janssen: Consultancy, Other: travel and accommodation expenses, Research Funding; GlaxoSmithKline: Consultancy; Celgene: Consultancy, Other: travel and accommodation expenses, Research Funding; Clovis Oncology: Other: Travel and accommodation expenses; Array BioPharma: Other: Travel and accommodation expenses; Amphivena Therapeutics: Other: Travel and accommodation expenses, Research Funding; Mesoblast: Consultancy; Novartis: Consultancy, Other: Travel and accommodation expenses, Research Funding; Amgen: Consultancy, Other: travel; Agios: Other: travel, Research Funding; Roche: Consultancy; Helsinn Therapeutics: Consultancy; Sunesis Pharmaceuticals: Other: Travel and accommodation expenses, Research Funding; Eisai: Other: Travel and accommodation expenses; CTI: Research Funding; Karyopharm Therapeutics: Research Funding; Bristol Myers Squibb: Consultancy; Astellas: Consultancy; Amgen: Consultancy; Agios: Consultancy, Research Funding; AbbVie: Consultancy, Other: travel and accommodations, Research Funding; Actinium: Consultancy; Onconova Therapeutics: Research Funding; Tensha Therapeutics: Research Funding. Desai: Janssen Research: Research Funding; Takeda, Bristol Myers Squibb, Agios: Consultancy, Membership on an entity's Board of Directors or advisory committees. Sallman: BMS: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Syndax: Membership on an entity's Board of Directors or advisory committees; Shattuck Labs: Membership on an entity's Board of Directors or advisory committees; Nemucore: Membership on an entity's Board of Directors or advisory committees; Lixte: Patents & Royalties: LB-100; Aprea: Membership on an entity's Board of Directors or advisory committees, Research Funding; Syntrix Pharmaceuticals: Research Funding; AbbVie: Membership on an entity's Board of Directors or advisory committees; Intellia: Membership on an entity's Board of Directors or advisory committees; Magenta: Consultancy; Agios: Membership on an entity's Board of Directors or advisory committees; Takeda: Consultancy; Novartis: Consultancy, Membership on an entity's Board of Directors or advisory committees; Incyte: Speakers Bureau; Kite: Membership on an entity's Board of Directors or advisory committees. Sweet: Gilead Sciences, Inc.: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Mablytics: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Astellas: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Bristol Myers Squibb: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Curis: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Syntrix Pharmaceuticals: Research Funding; berGenBio: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Pfizer: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Novartis: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; AROG: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Incyte: Research Funding. Komrokji: Geron: Consultancy; Servier: Consultancy, Honoraria, Speakers Bureau; CTI BioPharma, Innovent: Honoraria, Membership on an entity's Board of Directors or advisory committees; AbbVie: Consultancy, Honoraria, Speakers Bureau; Acceleron Pharma: Consultancy; Taiho Oncology: Consultancy, Membership on an entity's Board of Directors or advisory committees; PharmaEssentia, Takeda: Honoraria, Membership on an entity's Board of Directors or advisory committees; Novartis: Consultancy, Membership on an entity's Board of Directors or advisory committees; Jazz Pharmaceuticals: Consultancy, Honoraria, Speakers Bureau; Bristol Myers Squibb: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau. Lancet: Syntrix Pharmaceuticals: Research Funding; Astellas: Consultancy; Agios/Servio: Consultancy; Dava Oncology: Consultancy; Boxer Capital: Consultancy; Dedham Group: Consultancy; Jasper Therapeutics: Consultancy; Novartis: Consultancy; Jazz: Consultancy; BerGenBio: Consultancy; Millenium Pharma/Takeda: Consultancy; ElevateBio Management: Consultancy; Daiichi Sankyo: Consultancy; Celgene/BMS: Research Funding; AbbVie: Consultancy; Servier: Consultancy.

*signifies non-member of ASH