Session: 617. Acute Myeloid Leukemias: Biomarkers, Molecular Markers and Minimal Residual Disease in Diagnosis and Prognosis: Molecular Features and Response to Treatment in AML
Hematology Disease Topics & Pathways:
AML, adult, Acute Myeloid Malignancies, Diseases, Myeloid Malignancies
Methods: This is a retrospective, registry-based, multicenter analysis from the EBMT with the approval of the EBMT Acute Leukemia Working Party. Adult patients aged more than 18 years with a diagnosis of AML who received an allo-HSCT between 2014-2019, with an available pretransplant genetic profile by next generation sequencing (NGS) were included.
Results: We identified 362 allografted AML patients who had NGS performed before allo-HSCT. Most of these patients had de novo AML (85%), with a median age of 56 years (range: 18-78 years). The most frequent detectable mutations by frequency were DNMT3A (33%), FLT3 (27%), NPM1 (26%), TET2 (24%), RUNX1 (22%), BCOR (22%), NRAS (19%), WT1 (18%), ASXL1 (16%), IDH2 (16%,) STAG2 (16%), SRSF2 (14%), IDH1 (12%), TP53 (12%), KRAS (12%), and CEBPA (10%). By multiple correspondence analysis, two independent groups of co-occurring mutations were identified, the first group includes DNMT3A, NPM1 and FLT3, the second group includes ASXL1, SRSF2 and RUNX1. Outcome analysis was performed on the subset of 145 patients allografted in first complete remission with available data for the aforementioned six genes (DNMT3A, NPM1, FLT3, ASXL1, SRSF2 and RUNX1). Most of these patients had de novo AML (86%), with a median age of 54 years (range: 19-75 years). Patients received primarily myeloablative conditioning (58%) and peripheral blood stem cells (91%) from matched sibling donors (35%), matched unrelated (29%), and haploidentical donors (22%). Sixty five percent of these patients had intermediate-risk cytogenetics, while 32% were classified as adverse-risk. Median follow up calculated by reverse Kaplan-Meier was 2.7 years. Overall, the 2-year relapse incidence (RI), leukemia-free survival (LFS) and overall survival (OS) were 25%, 58%, and 68%, respectively. The 2-year RI, LFS and OS were 22%, 72% and 81%, respectively, for patients with NPM1 mutation, 20%, 65% and 70% for patients with RUNX1 mutation, 29%, 64% and 79% for patients with ASXL1 mutation, 33%, 57% and 65% for patients with DNMT3A mutation, 23%, 69% and 69% for patients with SRSF2 mutation, and 28%, 66% and 77% for patients with FLT3 mutation. When mutations were investigated in groups, the 2-year RI, LFS and OS were 22%, 72% and 81%, respectively, for patients with NPM1 mutation regardless of other co-mutations, 37%, 48% and 64% for patients with FLT3 and/or DNMT3A mutation, wild type NPM1, regardless of other co-mutations, 23%, 69% and 77% for patients with RUNX1 and/or ASXL1 and/or SRSF2 mutation and wild type NPM1, FLT3 and DNMT3A, and 21%, 50% and 57% for patients with all six genes unmutated. In conclusion, NGS at diagnosis can be extremely useful in risk stratification of AML patients undergoing allo-HSCT, potentially allowing adequate post-transplant interventions.
Disclosures: Labopin: Jazz Pharmaceuticals: Honoraria. Bug: Jazz: Honoraria; Pfizer: Consultancy; Celgene /BMS: Consultancy, Honoraria; Gilead: Consultancy, Honoraria; Novartis: Consultancy. Ribera: Pfizer: Membership on an entity's Board of Directors or advisory committees, Other: Research support; AMGEN: Membership on an entity's Board of Directors or advisory committees, Other: Research support; Shire: Membership on an entity's Board of Directors or advisory committees; Takeda: Membership on an entity's Board of Directors or advisory committees; Incyte: Membership on an entity's Board of Directors or advisory committees, Other: Research support; Novartis: Membership on an entity's Board of Directors or advisory committees. Schmid: Novartis Pharmaceuticals: Honoraria, Speakers Bureau; Neovii: Honoraria, Speakers Bureau. Ciceri: Kite Pharma: Consultancy. Mohty: Oncopeptides: Honoraria; GSK: Honoraria; Pfizer,: Honoraria; Adaptive Biotechnologies: Honoraria; Novartis: Honoraria; Astellas: Honoraria; Amgen: Honoraria; Takeda: Honoraria; Jazz Pharmaceuticals: Honoraria, Research Funding; Celgene: Honoraria; Bristol Myers Squibb: Honoraria; Gilead: Honoraria; Sanofi: Honoraria, Research Funding; Janssen: Honoraria, Research Funding.
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