Session: 622. Lymphomas: Translational–Non-Genetic: Poster I
Hematology Disease Topics & Pathways:
Research, Translational Research, immunology, Biological Processes, Technology and Procedures, profiling, pathogenesis
We have previously shown that high T-cell gene expression signature and high tumor-infiltrating lymphocyte (TIL) content translate to better survival in testicular but not in systemic DLBCL. We have also described a subpopulation of T-bet+FoxP3+ Tregs that have a significant adverse impact on survival in T-DLBCL but not in in systemic DLBCL.
In order to further characterize the differences in the TME between T- and systemic DLBCL, we used NanoString digital gene expression profiling to assess the expression of 770 immune response genes in 60 T-DLBCL and 84 systemic DLBCL samples. In addition, we used multiplex immunohistochemistry (mIHC) to characterize TILs, tumor-associated macrophages (TAMs), NK-cells, checkpoint molecules, cell proportions, and cell interactions in 80 T-DLBCLs and 192 systemic DLBCLs.
Unsupervised hierarchical clustering of the gene expression data revealed clusters of genes with differential expression between T- and systemic DLBCLs. In T-DLBCL, 11 HLA genes were downregulated and genes related to B-cell receptor (BCR) signaling upregulated compared to systemic DLBCL. All T-DLBCL patients that clustered among the patients with systemic DLBCL had high T-cell signature expression, and systemic DLBCL patients that clustered among patients with T-DLBCL had significantly higher Ki-67 expression (p=0.00085). Based on the mIHC data, we observed not only a significantly lower proportion of FoxP3+ Tregs altogether but also a higher proportion of T-bet+FoxP3+ double positive Tregs in T-DLBCL compared to systemic DLBCL, confirming our previous findings on the significant role of T-bet+FoxP3+ double positive Tregs in the T-DLBCL TME. We also observed less TAMs in T-DLBCL TME. Unsupervised hierarchical clustering of the cell interaction data verified that distinct interaction clusters are found and separate the patients into different subgroups. Interactions of cytotoxic T-cells were more commonly seen in T-DLBCL whereas interactions of PD-1 expressing cells and interactions between TILs and TAMs were more frequent in systemic DLBCL.
Taken together, our results reveal differences in the TME between T- and systemic DLBCL, and further emphasize the impact of immune escape and distinct immune cells in T-DLBCL. Future studies are ongoing in order to further characterize the cell-to-cell interactions, their association with survival, and TME differences between T- and systemic DLBCL, in particular non-GCB DLBCL.
Disclosures: Leppa: Bayer: Research Funding; Orion Pharma: Consultancy; Pfizer: Consultancy; Beigene: Consultancy; Genmab: Research Funding; BMS: Consultancy, Research Funding; Gilead Sciences: Consultancy, Honoraria; Roche: Consultancy, Research Funding; Novartis: Consultancy, Honoraria, Research Funding; Incyte: Consultancy, Honoraria; Nordic Nanovector: Research Funding.
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