Session: 704. Cellular Immunotherapies: Clinical: Poster II
Hematology Disease Topics & Pathways:
Clinical Trials, Clinical Research, Registries
A total of 175 infection events were detected, with 126 being detected by mNGS and 67 by routine (including 18 events detected by both,Figure 1A). It was found that 77.45% (79/102) of patients experienced an infection, with viral infections (72.55%, 74/102) being the most common infection type, followed by bacterial (26.47%, 27/102) and fungal (9.80%, 10/102) infections (Figure 1B-E). Stenotrophomonas maltophilia (4.90%) was the most common bacterium detected in febrile CAR-T recipients. Among these bacterial infections, mNGS detected a greater number of infection events and a wider variety of bacterial species. Human beta herpes virus 5 (CMV) was the most common virus, detected in 42.16% (43 in 102) patients.An average of 1.05 mNGS tests detected more microbes that were not detected by a combination of 16.10 routine tests. Fungal infection probability was 5.26% in mild CRS and 23.08% in severe CRS (Figure 1F, p<0.05). Fungal infection density was 0.05 in mild CRS and 0.23 in severe CRS (Figure 1G, p<0.05). In addition, bacterial infection density was higher in severe CRS (0.70 vs 0.30, Figure 1G, p<0.05). Multiple infection, defined as being infected with 2 or more microbes of any type, occurred in 53.95% (41 in 76) of patients in the mild CRS and 61.54% (16 in 26) of patients in the severe CRS. Complex infections, defined as being infected with 2 or more microbes of different types, occurred in 25.00% (19 in 76) of patients in the mild CRS and 38.46% (10 in 26) of patients in the severe CRS (Figure 1H). These findings indicate that complex infection was slightly more common in severe CRS.
This study, at first time, reveals that in most febrile CAR-T recipients, infection is a major etiology, with 77.45% (79/102) patients having at least one species of microbe in their peripheral blood. Of all the microbic species, viral infection was the most common, particularly in patients with mild CRS, in contrast to patients with severe CRS who had an increased risk of fungal infection. Due to its enhanced spectrum of microbiological diagnosis and efficiency, mNGS could represent an important complement to routine tests in standard clinical application.
Disclosures: No relevant conflicts of interest to declare.
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