62nd ASH Annual Meeting and Exposition: 201. Granulocytes, Monocytes, and Macrophages

Oral and Poster Abstracts
Oral

Adult, Biological, HSCs, AML, apoptosis, bone marrow, Diseases, bioengineering, iPSCs, cell regulation, Bone Marrow Failure, Therapies, Pediatric, Genetic Disorders, white blood cells, Biological Processes, enzyme inhibitors, gene therapy, Technology and Procedures, Young Adult, immune cells, Cell Lineage, immunotherapy, Study Population, gene editing, Clinically relevant, Myeloid Malignancies, hematopoiesis, immune mechanism, inflammation, stem cells, microenvironment, RNA sequencing, pathways

Moderators:

Julia T Warren, MD, PhD, Washington University in St. Louis and Jan Palmblad, MD, PhD, Karolinska Institutet

Disclosures:

No relevant conflicts of interest to declare.

1:30 PM

682

Efficient Correction of HAX1 Mutations in Primary HSPCs of Severe Congenital Neutropenia Patients Using CRISPR/CAS9 GENE-Editing

Malte U Ritter¹^*, Benjamin Secker¹^*, Masoud Nasri, MSc¹^*, Maksim Klimiankou¹^*, Benjamin Dannenmann, MSc²^*, Diana Amend³^*, Jeremy Haaf, Msc¹^*, Perihan Mir¹^*, Regine Bernhard¹^*, Ingeborg Steiert¹^*, Cornelia Zeidler^4,5^*, Karl Welte^6,7 and Julia Skokowa, MD, PhD¹

¹Department of Oncology, Hematology, Immunology, Rheumatology and Pulmonology, University Hospital Tuebingen, Tuebingen, Germany
²Department of Oncology, Hematology, Immunology, Rheumatology and Pulmonology, University Hospital Tuebingen, Tuebingen, Baden-Wuerttemberg, Germany
³Helmholtz Zentrum München, Ludwigs-Maximilian Universität München, München, Germany
⁴SCNIR, Hannover Medical School, Hannover, Germany
⁵Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, Hannover, Germany
⁶Pediatric Hematology and Oncology, University Children Hospital Tuebingen Germany, Tuebingen, Germany
⁷Severe Chronic Neutropenia International Registry, Department of Molecular Hematopoiesis, Medical School Hannover, Hannover, Germany

1:45 PM

683

CRISPR Mediated ELANE Single-Allele Knock-out Restores Proliferation and Myeloid Differentiation of Neutropenia Patient Derived BM HSCs

Peter Sabo¹^*, Vahagn Makaryan, MD¹, Tanoya Poulsen¹^*, Lital Povodovski²^*, Yosef Dicken²^*, Asael Herman, PhD²^*, Rafi Emmanuel²^* and David C. Dale, MD¹

¹Department of Medicine, University of Washington, Seattle, WA
²Emendo Biotherapeutics, Ness Ziona 741400, Israel

2:00 PM

684

Transcriptome Analysis of Severe Congenital Neutropenia Associated Leukemia with Different Types of RUNX1 Mutations in an iPSC-Based Model

Benjamin Dannenmann, MSc¹^*, Benedikt Oswald¹^*, Malte U Ritter¹^*, Masoud Nasri, MSc¹^*, Regine Bernhard¹^*, Cornelia Zeidler²^*, Karl Welte³, Julia Skokowa, MD, PhD¹ and Maksim Klimiankou¹^*

¹Department of Oncology, Hematology, Immunology, Rheumatology and Pulmonology, University Hospital Tuebingen, Tuebingen, Germany
²SCNIR, Hannover Medical School, Hannover, Germany
³Pediatric Hematology and Oncology, University Children Hospital Tuebingen Germany, Tuebingen, Germany

2:15 PM

685

Systems-Level Analysis of the Immune Repertoire in Neutropenia Reveal Arrested NK Cell Differentiation and Exhaustion

Ebba Sohlberg¹^*, Aline Pfefferle, Ph.D.²^*, Eivind Heggernes Ask, MD³^*, Astrid Tschan-Plessl, MD⁴^*, Benedikt Jacobs, MD⁵^*, Suzanne Lorenz, PhD⁶^*, Stephan Meinke⁷^*, Petter Hoglund, MD, PhD⁸^*, Mikael Sundin, MD, PhD⁹, Goran Karlsson, MD.PhD.¹⁰^*, Jan Palmblad, MD, PhD¹¹ and Karl-Johan Malmberg, MD, PhD¹²^*

¹CIM, Karolinska Institutet, Stockholm, Sweden
²Karolinska Institute, Stockholm, Sweden
³Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, 0376, Norway
⁴University Hospital of Basel, Basel, CHE
⁵Department of Hematology and Oncology, University Hospital Erlangen, Erlangen, Germany
⁶Genomics Core Facility, Department of Core Facilities, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
⁷Center for Hematology and Regenerative Medicine, Karolinska Institutet, Stockholm, Sweden
⁸Department of Medicine, Karolinska Institutet, stockholm, Sweden
⁹Division Pediatric Hematology, Immunology and HCT, Karolinska University Hospital, Stockholm, SWE
¹⁰Astrid Lindgren´s Childrens Hospital, Stockholm, Sweden
¹¹Karolinska Institutet, Stockholm, Sweden
¹²Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway

2:30 PM

686

Myeloid-Specific SHP-2 Ablation Induces Robust Anti-Tumor Immunity That Is Not Further Enhanced By PD-1 Blockade

Anthos Christofides, MD¹^*, Natalia M Tijaro-Ovalle, MD¹^*, Halil-Ibrahim Aksoylar, PhD¹^*, Rinku Pal, MS²^*, Abdelrahman AA Mahmoud³^*, Laura Strauss, PhD²^*, Nikolaos Patsoukis, PhD¹^* and Vassiliki A Boussiotis, MD, PhD²

¹Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA
²Hematology-Oncology, Beth Israel Deaconess Medical Center, Boston, MA
³Faculty of Biosciences, Heidelberg University, Heidelberg, Germany

2:45 PM

687

Frequency and Functional Analysis of Myeloid-Derived Suppressor Cells (MDSCs) in the Peripheral Blood and Bone Marrow of Patients with Chronic Idiopathic Neutropenia (CIN)

Nikoleta Bizymi, MD, MSc¹^*, Athina Damianaki¹^*, Maria Velegraki, MD, PhD¹^*, Konstantina Zavitsanou, MSc¹^*, Anastasios Karasachinidis¹^*, Anthie Georgopoulou, PhD¹^*, Irene Mavroudi, PhD¹^*, John Sperelakis, MD²^*, George Kontakis, MD, PhD²^*, Charalampos Pontikoglou, MD, PhD¹^* and Helen A. Papadaki, MD, PhD¹

¹Hemopoiesis Research Laboratory, School of Medicine, University of Crete and Department of Hematology, University Hospital of Heraklion, Heraklion, Greece
²Department of Orthopedics, University Hospital of Heraklion, Heraklion, Greece

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^*signifies non-member of ASH

201.O1.6 201. Granulocytes, Monocytes, and Macrophages