Session: 603. Oncogenes and Tumor Suppressors: Poster I
Hematology Disease Topics & Pathways:
Diseases, Non-Biological, Therapies, chemotherapy, B-Cell Lymphoma, Biological Processes, DNA damage, Lymphoid Malignancies
Methods: Immunohistochemistry staining (IHC) for AMOT expression was performed on tissues of 52 newly diagnosed DLBCL and 25 reactive hyperplasia cases (controls). Peripheral blood mononuclear cells (PBMCs) were isolated from three healthy volunteers. Expression levels of AMOT mRNA and protein in DLBCL cells and PBMCs (controls) were detected by RT-PCR and western blot. Stable overexpressed cell lines (LV-AMOT) were generated by lentiviral infection. Effects of cell viability, cycle and apoptosis were assessed by cell counting kit-8 assay, propidium iodide and Annexin V-PE/7AAD staining, respectively. Moreover, we examined the effect of AMOT on the key protein of DDR by western blot.
Results: The percentage of AMOT-positive cells in DLBCL samples was statistically less than in reactive hyperplasia of lymph nodes tissues (Fig. 1A). The expression of AMOT was negatively associated with serum lactate dehydrogenase (LDH, P=0.023) and international prognostic index (IPI) score (P=0.017). Furthermore, Kaplan-Meier analysis indicated that the median survival of patients was significantly longer in AMOT-positive group than AMOT-negative group (Fig. 1B). DLBCL cells (LY1, LY8 and Val) exhibited remarkably lower mRNA as well as protein levels of AMOT compared to the control groups (Fig. 1C and D). AMOT-overexpression caused substantial growth suppression (Fig.1E) and increased proportion of cells in the G1 phase, with a concomitant decrease in the S phase of DLBCL cells (Fig. 1F). However, no significant change in the apoptosis rate after AMOT upregulation was observed.
The effect of AMOT on the activating phosphorylation of several key checkpoint proteins was examined by western blot assay. AMOT upregulation attenuated the activating phosphorylation of ATM(Ser1981), ATR (Ser428), Chk1 (Ser345), Chk2 (Thr68), and H2AX (Ser139) (Fig. 1G). However, the mRNA expression level of these genes did not change.
To investigate the effect of AMOT on the chemosensitivity of DLBCL cells, we incubated the DLBCL cells with doxorubicin for different concentrations or different time. The LV-AMOT group showed increased sensitivity to doxorubicin compared to the LV-Con group (Fig. 1H). Compared with the control, the LV-AMOT group showed an increased accumulation of cells in the G1 phase (Fig. 1I) and improved apoptosis rate (Fig. 1J). Western blotting showed that AMOT upregulation attenuated the activating phosphorylation of ATM (Ser1981)、ATR (Ser428)、Chk1 (Ser345)、Chk2 (Thr68) and H2AX (Ser139) after doxorubicin (160nM) treatment for 24 hours(Fig. 1K).
Conclusion: AMOT acts as a tumor suppressor via inhibition of the DDR, thus reducing the viability while increasing chemosensitivity in DLBCL. AMOT may be a potential target for DLBCL patients with doxorubicin resistance.
Disclosures: No relevant conflicts of interest to declare.