Program: Oral and Poster Abstracts
Session: 101. Red Cells and Erythropoiesis, Structure and Function, Metabolism, and Survival, Excluding Iron: Poster II
Methods:To get further insight into the different responses to Eculizumab treatment among patients, we have generated a PNH cohort including 12 PNH patients treated with Eculizumab. All patients included in the study had previously a minimum of ≥12 months on treatment with the drug (ten receiving 900 mg every 14 days, and two patients on 1200 mg every 14 days because breakthrough hemolysis). We have collected samples during two Eculizumab cycles (4 weeks) at different points during treatment: before and after Eculizumab injection and in the intermediate week. We have searched for correlations between clinical and hematological parameters, complement genetic findings, and deposition of C3 fragments on erythrocytes and plasma levels of complement activation components.
Results: We report the CR1 genotypes, complement and hematological determinations in 12 PNH patients during their treatment with Eculizumab. We have established that concentrations of free Eculizumab above 110 µg/mL completely inhibit serum hemolytic activity. Consistent with this, in our patient cohort, most patients with levels of free Eculizumab close or below 110 µg/mL exhibited traces of C5b9 and/or CH50. In general, lowest levels of free Eculizumab correlate with higher levels of C5b9. Our results indicate that proper monitoring of PNH patients would be useful to optimize Eculizumab dosage. We have 3 patients that showed symptoms of intravascular hemolysis (high LDH and hemosiderinuria) despite having a negative CH50 accompanied with sufficient free Eculizumab levels. Further studies are currently being done to elucidate the causes. We have found a correlation between CR1 haplotype, red cells opsonization and C3 plasma levels: H/L individuals present higher % of C3 positive erythrocytes and low C3 plasma levels. Two of these patients also had low C4 plasma levels, one of them presenting also low levels of FH. These individuals are currently being studied to determine whether these low levels are due to genetic factors or consumption. We found a correlation between levels of bilirubin and % of reticulocytes; patients who had more than the 30% of their erythrocytes opsonized presented higher percentage of reticulocytes and bilirubin in plasma, which could be taken as indicatives of slight extravascular hemolysis. In this regard, individuals carrying the CR1 L allele might require special attention.
Disclosures: Ojeda: Alexion Pharmaceuticals: Consultancy , Membership on an entity’s Board of Directors or advisory committees , Speakers Bureau . Fores: Alexion Pharmaceuticals: Consultancy , Speakers Bureau . Villegas-Martinez: Alexion Pharmaceuticals: Consultancy , Speakers Bureau . Rodriguez-Cordoba: Alexion Pharmaceuticals: Consultancy , Membership on an entity’s Board of Directors or advisory committees , Speakers Bureau .
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