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321.P1.59 Blood Coagulation and Fibrinolytic Factors: Poster I

Program: Oral and Poster Abstracts
Type: Poster
Saturday, December 4, 2010: 5:30 PM-7:30 PM
Hall A3/A4 (Orange County Convention Center)
Role of the N-Terminal Amino Acid Region of Factor Va Light Chain In Prothrombinase Assembly and Function

John L. Vaughn, BS1*, Jamila Hirbawi, PhD2*, Michael A. Bukys, PhD3* and Michael Kalafatis, PhD4

1College of Medicine, The Ohio State University, Columbus, OH
2Molecular Cardiology, Cleveland Clinic, Cleveland, OH
3Stem Cell Biology and Regenerative Medicine, Cleveland Clinic, Cleveland, OH
4Dept. of Chemistry, Cleveland State University, Cleveland, OH

Poster Board Number: I-107
Nematode Antocoagulant Protein c2 (NAPc2) Interferes with Factor Xa Dimerization: Structural Alteration of Factor Xa Upon Dimerization

Rima Chattopadhyay1*, Tilen Koklic1*, Rinku Majumder, PhD2 and Barry R. Lentz1*

1Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC
2Biochemistry & Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC

Poster Board Number: I-108
Identification of a Novel Coagulation Factor X Compound Heterozygous Mutation Associated with Differential Initiating Clotting Pathway Function

Amanda L Vanden Hoek, BSc1*, Kimberley Talbot, MPhil1*, Isis Carter, BSc2*, Linda Vickars, MD, FRCP(C)3, Cedric John Carter, MD4, Ross T.A. MacGillivray, PhD5* and Ed L.G. Pryzdial, PhD1

1Research and Development, Canadian Blood Services, Centre for Blood Research/Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada
2Research and Development, Canadian Blood Services, Centre for Blood Research/Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada
3Hematology, St. Paul's Hospital, Providence Health Care, Medicine/Hematology, University of British Columbia, Vancouver, BC, Canada
4Centre for Blood Research/Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada
5Centre for Blood Research/Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada

Poster Board Number: I-109
Lyso-Sulfatide Binds Factor Xa and Potently Inhibits Thrombin Generation

Subramanian Yegneswaran, PhD1*, Yajnavalka Banerjee, PhD2*, Jose A. Fernandez, MD, PhD1, Hiroshi Deguchi, MD, PhD2* and John H. Griffin, PhD2

1Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA
2Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA

Poster Board Number: I-110
Calcium Ionophore-Induced Tissue Factor (TF) Decryption Induces TF Immobilization Into Lipid Rafts and Negative Regulation of TF Procoagulant Activity

Narcis I. Popescu, PhD*, Cristina Lupu, PhD* and Florea Lupu, PhD

Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK

Poster Board Number: I-111
Tissue Factor (TF) Activation by Antithymocyte Globulin Is Inhibited by a Novel Monoclonal Antibody Specific for Reduced TF Mutated at the Allosteric Cys186-Cys209 Disulfide

Florian Langer, MD1*, Brigitte Spath1*, Francis Ayuketang Ayuk, MD2*, Nicolaus Kroeger, MD2*, Barbara Eifrig, MD1*, Carsten Bokemeyer, MD1* and Wolfram Ruf, MD3

1Hematology and Oncology, University Hospital Eppendorf, Hamburg, Germany
2Bone Marrow Transplantation, University Hospital Eppendorf, Hamburg, Germany
3Immunology and Microbial Sciences, The Scripps Research Institute, La Jolla, CA

Poster Board Number: I-112
Increased Platelet Binding of NN1731, a Factor VIIa Variant with Enhanced Tissue Factor-Independent Activity

Maureane R. Hoffman, MD, PhD1, Egon Persson, PhD2*, Mirella Ezban2* and Dougald M. Monroe III, PhD3

1Pathology and Lab. Medicine, Durham VA Medical Center, Durham, NC
2Haemostasis Biochemistry, Novo Nordisk, Måløv, Denmark
3Hematology/Oncology, University of North Carolina, Chapel Hill, NC

Poster Board Number: I-113
TFPI Antagonist Aptamer ARC19499 Is a Procoagulant In Vitro and In Vivo

Kathleen E. McGinness, PhD*, Emily K. Waters, PhD, Ryan M. Genga*, Karen A. Olson, PhD*, Jennifer A. Nelson, PhD*, Jeffrey C. Kurz, PhD and Robert G. Schaub, PhD

Archemix Corporation, Cambridge, MA

Poster Board Number: I-114
Evaluation of Non-Proteolytic Functions of Murine Alternatively Spliced Tissue Factor 

Richard Godby1*, Yascha van den Berg, MD2*, Ramprasad Srinivasan, PhD3*, Evgeny Ozhegov, MD, PhD3*, Henri H Versteeg, PhD2* and Vladimir Bogdanov, PhD3

1B.S./M.D. Dual Admissions Program, Division of Hematology/Oncology, Internal Medicine, University of Cincinnati College of Medicine / College of Engineering & Applied Science, Cincinnati, OH
2Experimental Vascular Medicine, Leiden University Medical Center, Leiden, Netherlands
3Hematology/Oncology, Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH

Poster Board Number: I-115
Anthracycline Treatment of the Monocytic Leukemia Cell Line THP-1 Increases Phosphatidylserine Exposure and Tissue Factor Activity

Jeremiah Boles, MD, Sam Glover, BS*, A. Phillip Owens III, PhD*, Rachel Hollingsworth*, Raj S. Kasthuri, MD, MBBS, Nigel S. Key, MBChB and Nigel Mackman, PhD

Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC

Poster Board Number: I-116
Protein S Released From Stimulated Platelets Has Anticoagulant Activity Independent of Activated Protein C and Tissue Factor Pathway Inhibitor (TFPI)

Mary J. Heeb, PhD and Erning Duan, MS*

Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA

Poster Board Number: I-117
Covalent End-Labeling of Polyphosphate Facilitates Studies of Its Procoagulant Activities and Development of Enhanced Agents to Treat Bleeding

Sharon H. Choi, MS1*, Rebecca L Davis-Harrison, PhD2*, Stephanie A. Smith, DVM, MS1, Julie N. Collins, MS2*, Chad M Rienstra, PhD3* and James H. Morrissey, PhD1

1Dept. of Biochemistry, Univ. of Illinois College of Medicine, Urbana, IL
2Department of Biochemistry, University of Illinois, Urbana, IL
3Department of Chemistry, University of Illinois, Urbana, IL

Poster Board Number: I-118
Characterizing Membrane-Bound Forms of Coagulation Factors at Atomic Resolution: Exploring Differences In Membrane Affinity

Mark J Arcario1* and Emad Tajkhorshid2*

1Center for Biophysics and Computational Biology, College of Medicine, University of Illinois at Urbana-Champaign, Champaign, IL
2Center for Biophysics and Computational Biology, Department of Biochemistry, College of Medicine, University of Illinois at Urbana-Champaign

Poster Board Number: I-119
Short Polyphosphates Inhibit the Procoagulant Activities of Long-Chain Polyphosphate: A Novel Role for Platelet-Derived Pyrophosphate?

Stephanie A. Smith, DVM, MS1 and James H. Morrissey, PhD2

1Biochemistry, University of Illinois College of Medicine, Urbana, IL
2University of Illinois College of Medicine, Urbana, IL

Poster Board Number: I-120
Structural Insights Into How Clotting Proteins with GLA Domains Bind to Membrane Surfaces

Rebecca L Davis-Harrison, PhD1*, Narjes Tavoosi1*, Mary Clay2*, John M Boettcher, Ph.D.2*, Chad M Rienstra, Ph.D2* and James H. Morrissey, PhD3

1Department of Biochemistry, University of Illinois, Urbana, IL
2Department of Chemistry, University of Illinois, Urbana, IL
3Dept. of Biochemistry, Univ. of Illinois College of Med., Urbana, IL

Poster Board Number: I-121
A Novel Dimeric Inhibitor That Targets Dimeric ß2GPI In ß2GPI/Anti-ß2GPI Antibody Complexes

Natalia Beglova, Alexey Kolyada*, Chang-Jin Lee* and Alfredo De Biasio*

Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA

Poster Board Number: I-122
Characterization of the Procoagulant Activity of Two Different Microparticle Species

Ludwig Traby1*, Marietta Kollars1*, Khalid Shoumariyeh1*, Ansgar Weltermann, MD2*, Paul Alexander Kyrle1, Sabine Eichinger1* and Daniel Lechner1*

1Department of Medicine I, Medical University of Vienna, Vienna, Austria
2Department of Internal Medicine I, Hospital of the Elisabethinen, Linz, Austria

Poster Board Number: I-123
The Endothelial Protein C Receptor Supports TF-VIIa-Xa Ternary Complex Signaling through Protease-Activated Receptors

Jennifer Disse, Ph.D.1*, Helle Heibroch Petersen2*, Katrine S. Larsen2*, Egon Persson, PhD2*, Naomi Esmon3*, Charles Esmon, PhD3, Lars C. Petersen2*, Luc Teyton1* and Wolfram Ruf, MD1

1Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA
2Novo Nordisk, Måløv, Denmark
3Oklahoma Medical Research Foundation, Oklahoma City, OK

Poster Board Number: I-124
Role of Rab Proteins In Endocytosis and Trafficking of Factor VIIa and Endothelial Cell Protein C Receptor In Endothelial Cells

Ramesh C Nayak, Ph.D.*, Shiva Keshava, Ph.D.*, Usha R Pendurthi, Ph.D. and L. Vijaya Mohan Rao, Ph.D.

Center for Biomedical Research, The University of Texas Health Science Center at Tyler, Tyler, TX

Poster Board Number: I-125
Carbon Monoxide-Releasing Molecule-Derived CO Regulates Tissue Factor, Plasminogen Activator Inhibitor Type 1, and Thrombomodulin Production by Human Endothelial Cells

Keiko Maruyama1*, Eriko Morishita, MD, PhD2*, Shigeki Ohtake, MD, PhD2, Akihiro Yachie, MD, PhD3* and Shinji Nakao, MD, PhD4

1Clinical Laboratory Science, Kanazawa University, Kanazawa, Japan
2Clinical Laboratory Science,, Kanazawa University, Kanazawa, Japan
3Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan
4Cellular Transplantation Biology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan

Poster Board Number: I-126
Structural and Mutational Analysis of Canonical Loop Residues In Protease Nexin 2 Involved In Factor XIa and Trypsin Inhibition

Duraiswamy Navaneetham1*, Dipali Sinha2* and Peter N. Walsh, MD, PhD3

1Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA
2Thrombosis Research Center, Temple University School of Medicine, Philadelphia
3Thrombosis Research Center, Departments of Medicine and Biochemistry, Temple University School of Medicine, Philadelphia, PA

Poster Board Number: I-127
Localization of Ligand-Binding Exosites In the Catalytic Domain of Factor XIa

Ya-Chi Su1*, Dipali Sinha1* and Peter N. Walsh, MD, PhD2

1Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA
2Thrombosis Research Center, Departments of Medicine and Biochemistry, Temple University School of Medicine, Philadelphia, PA

Poster Board Number: I-128
FXII Antisense Oligonucleotide Mediated Depletion Results In Effective Anticoagulation without Bleeding Risk

Jeff R. Crosby, PhD1, Dacao Gao2*, Chenguang Zhao1*, Chris May1*, Robert MacLeod, Ph.D.1* and Brett P. Monia, PhD2

1Drug Discovery, ISIS Pharmaceuticals, Carlsbad, CA
2ISIS Pharmaceuticals, Carlsbad, CA

Poster Board Number: I-129
Activation of Factor XI and the Contact Proteases by Products of Prothrombin Activation

Anton Matafonov, MD1, Suryakala Sarilla, PhD2*, Mao-fu Sun1*, John P. Sheehan, MD3, Vladimir Serebrov, MD, PhD, DMSci4*, Ingrid M. Verhamme, PhD1* and David Gailani, MD1

1Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN
2Department of Pathology, Vanderbilt University, Nashville, TN
3Medicine/Hematology-Oncology, University of Wisconsin-Madison, Madison, WI
4Department of Biochemistry and Molecular Biology, Siberian State Medical University, Tomsk, Russia

Poster Board Number: I-130
Procoagulant Activity of Antifibrinolytic Agents; A Novel Hemostatic Mechanism of Tranexamic Acid and Epsilon-Aminocaproic Acid

Kenichi Ogiwara, MD, PhD*, Keiji Nogami, MD, PhD*, Katsumi Nishiya, MD, PhD*, Nobuyuki Tsujii, MD* and Midori Shima, MD, PhD

Department of Pediatrics, Nara Medical University, Kashihara, Japan

Poster Board Number: I-131
Host Fibrinogen and the S. Aureus-Encoded Procoagulant Vwbp Are Context-Dependent Determinants of Bacterial Virulence

Joni M Prasad1*, Xinli Du, MD, PhD1*, Eric S. Mullins, MD2, Senad Divanovic, PhD3*, Kathryn E Talmage1*, Keith W Kombrinck1*, Alice G Cheng4*, Olaf Schneewind, MD, PhD4*, Matthew J. Flick, PhD1 and Jay L. Degen, PhD2

1Experimental Hematology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH
2Cancer and Blood Diseases Institute, Cincinnati Children's Research Foundation, Cincinnati, OH
3Molecular Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH
4Microbiology, Univeresity of Chicago, Chicago, IL

Poster Board Number: I-132
Herpesviruses Enhance Fibrinogen Clot Lysis

Edwin S Gershom*, Amanda Vanden Hoek*, Michael R Sutherland, PhD* and Ed L.G. Pryzdial, PhD

Canadian Blood Services, Vancouver, BC, Canada

Poster Board Number: I-133
Fibrinogen residue γAla341 Is Necessary for Calcium Binding and ‘A-a’ interaction

Rojin Park, MD1*, Lifang Ping1*, Jaewoo Song, MD2, Sung-Yu Hong, PhD3*, Jong-Rak Choi, MD2*, Oleg V Gorkun, PhD1* and Susan T Lord, PhD1*

1Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC
2Laboratory Medicine, Yonsei Univ. Health System, Seoul, South Korea
3Cardiovascular Product Evaluation Center, Yonsei University Health System, Seoul, South Korea

Poster Board Number: I-134
Differential Effects of Heparins on the Nanostructure of Fibrin Clot

Francois Caton, PhD1*, Christelle Yeromonahos, MSc2*, Raphael Marlu, MD3* and Benoit Polack, MD, PhD4*

1Laboratoire de Rheologie, CNRS UMR 5520, University of Grenoble, Grenoble, France
2Laboratoire de Rheologie, CNRS UMR 5520, University of Grenoble, Grenoble
3Department of Hematology, Centre Hospitalier Universitaire, Grenoble, France
4TIMC-IMAG, TheREx, CNRS UMR 5525, University of Grenoble, Grenoble, France

Poster Board Number: I-135
From Fibrinogen to Fibrin: Dynamic Light Scattering to Probe the Mechanism of Protofibril Assembly Into Fibers

Lihong Huang1* and Susan T Lord, PhD2*

1Pathology and Lab Medicine, University of North Carolina at Chapel Hill, Raleigh, NC
2Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC

Poster Board Number: I-136

*signifies non-member of ASH