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Ex Vivo Expansion, Mobilization and Engraftment Poster II

Monday, December 8, 2008: 5:30 PM-7:30 PM
Hall A (Moscone Center)
Mimicking the Hematopoietic Niche Microenvironment Provides a Novel Strategy for Expansion of Hematopoietic and Megakaryocyte-Progenitor Cells from Cord Blood

Varda R Deutsch, PhD1, Einav Hubel, MSc1*, Sigi Kay, PhD1*, Tal Ohayon, MSc1*, Ben-Zion Katz, PhD1*, Elizabeth Naparstek, MD1 and Dan Grisaru, MD2*

1Hematology and Bone Marrow Transplantation, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel
2Obstetrics and Gynecology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel

Aneuploidy in Clinical-Grade Mesenchymal Stem Cells Is Not Linked to Cell Transformation and Not Related to Culture Method

Karin Tarte, PhD1*, Jean-jacques Lataillade, MD, PhD2*, Loïc Fouillard, MD, PhD3*, Julien Gaillard4*, Martine Becker, MD5*, Delphine Monnier, PhD1*, Helene Rouard, PhD6*, Andrei Tchirkov, MD, PhD7*, Jean-Paul Vernant, Pr8*, Norbert C Gorin, Pr9, Patrick Gourmelon, Pr10* and Luc Sensebe, MD, PhD11*

1Inserm U917-MICA, Faculté de Médecine de Rennes, Rennes, France
2CTSA, Clamart, France
3Hematology, AP-HP Hopital Saint-Antoine, Paris, France
4Research department, EFS Centre Atlantique & EA3855, Tours, France
5Futurebiolab, Genève, Switzerland
6Thérapie Cellulaire, EFS Ile de France, Créteil, France
7Service de Cytogénétique Médicale, CHU de Clermont-Ferrand and EA3846, Clermont-Ferrand, France
8Service d'Hématologie Clinique, Hôpital Pitié-Salpêtrière, Paris, France
9Hematology-Oncology, Hopital Saint-Antoine and Universite Pierre et Marie Curie UPMC, Paris, France
10IRSN, France
11Research department, EFS Centre Atlantique & EA3855, 37000 Tours, France

The Impact of Rhg-CSF on the LFA-1 Conformation of CD4+T Cells in Mobilized Hematopoietic Stem Cell Allografts

Chunji Gao, Weihua Chen*, Fei Wang*, Meng Li*, Haiyan Zhu* and Wanming Da*

Dept. of Hematology, Chinese PLA General Hospital, Beijing, China

Implementation of  Combined Storage of the Hematopoietic Cord Blood Cells and Fetal Mesenchymal Stromal Cells (umbilical cord stromal cells, UCSC) from the Same Donors in Warsaw Cord Blood Bank

Zygmunt Pojda, M.D., Ph.D1, Eugeniusz K Machaj, Ph.D.2*, Marzena Korczak, M.Sci.1*, Aleksandra Kopera, M.Sci.1*, Paulina Adamczyk-Wojciechowska, M.Sci.1*, Jadwiga Kuczynska-Sicinska, M.D., Ph.D.3*, Romuald Debski, M.D., Ph.D.4*, Krzysztof Czajkowski, M.D., Ph.D.3* and Monika Turkowska, M.Sci.2*

1Department of Experimental Hematology and Cord Blood Bank, Oncology Center-Institute, Warsaw, Poland
2Department of Regenerative Medicine, WIHE Institute of Hygiene and Epidemiology, Warsaw, Poland
3Department of Obstetrics and Gynecology, Duchess Anna Mazowiecka Public Teaching Hospital, Medical University of Warsaw, Warsaw, Poland
4Department of Obstetrics and Gynecology, Medical Center for Postgraduate Education, Orlowski Hospital, Warsaw, Poland

Correlation of Cytokines Levels and Adhesion Molecules Expression with Stem Cell Mobilization Efficacy in Healthy Donors

Daniel Lysák, MD1*, Alexandra Jungová, MD1*, Jindra Vrzalová, M.Sc.2*, Luboš Holubec, MD, PhD2* and Vladimir Koza, MD3*

1Dept. of Hematology and Oncology, University Hospital Pilsen, Pilsen, Czech Republic
2Immunoanalytic Laboratory, University Hospital Pilsen, Pilsen, Czech Republic
3Czech National Marrow Donor Registry, Pilsen, Czech Republic

Stem Cell Mobilization Following Initial Therapy with Lenalidomide and Dexamethasone in Patients with Newly Diagnosed Multiple Myeloma

Shaji Kumar, MD, Martha Lacy*, Angela Dispenzieri*, Suzzanne Hayman*, Francis Buadi*, David Dingli*, Teresa Miceli*, Mark Litzow*, S.Vincent Rajkumar* and Morie Gertz*

Hematology, Mayo Clinic, Rochester, MN

Best Approach for Harvesting Bone Marrow to Maximize TNC and CD34+ Cell Counts

Nalini K Pati, MBBS, MD, DNB1*, Frances Garvin, BSc1*, Vicki Antonenas, BSc1*, Ian Kerridge, FRACP, FRCPA, PhD1*, Kenneth F Bradstock, MB, PhD, FRACP, FRCPA2 and David J Gottlieb, MD, FRACP, FRCPA3*

1Haematology, ICPMR, Westmead Hospital, Westmead Hospital, Sydney, Australia
2Department of Haematology, Westmead Hospital, Sydney, NSW 2145, Australia
3University of Sydney, Sydney, Australia

G-CSF Mediated Decrease in Bone-Marrow SDF-1 Level Is Neutrophil Dependent but CD26 Independent

Pratibha Singh, PhD1*, Seiji Fukuda, MD, PhD2, Janardhan Sampath, PhD1* and Louis M. Pelus, PhD1

1Microbiology and Immunology and the Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN
2Dept. of Pediatrics, Shimane Univ., Izumo, Japan

Successful Harvesting of Peripheral Hematopoietic Stem Cells after Induction Treatment with Bortezomib, Adriamycin, Dexamethasone (PAD) in Patients with Newly Diagnosed Multiple Myeloma (MM)

Hartmut Goldschmidt1*, Henk M. Lokhorst2, Uta Bertsch1*, Bronno van der Holt3*, Laila el Jarari3*, Hans-Jürgen Salwender4*, Sonja Zweegman2, Edo Vellenga2, Jörg Schubert4*, Igor W. Blau4*, Asiong Jie2*, Berna Beverloo2*, Dirk Hose1, Helgi van de Velde5*, Martyn Ronald Schaafsma2, Marie Jose Kersten2*, Michel Delforge6, Okke de Weerdt2*, Rene Van der Griend2*, Pierre Wijermans2, Hans Martin4*, Christof Scheid4, Mathias Hänel4, Ingo G.H. Schmidt-Wolf4* and Pieter Sonneveld for the HOVON and GMMG group7*

1GMMG and University of Heidelberg, Heidelberg, Germany
2HOVON, Netherlands
3HOVON Data Center, Netherlands
4GMMG, Germany
5Johnson&Johnson, Belgium
6HOVON, Belgium
7HOVON and Erasmus MC, Rotterdam, Netherlands

Short-Term Ex Vivo Expansion of CD133+ Cells by Co-Culture with Mesenchymal Stem Cells:  Role of SDF-1/CXCL12

Sarah Vaiselbuh, MD1*, Jeffrey Michael Lipton, MD, PhD2 and Johnson M. Liu, MD1

1Department of Pediatrics, The Feinstein Institute for Medical Research, Manhasset, NY
2Department of Pediatrics, Division of Pediatric Hematology/Oncology and Stem Cell Transplantation, Schneider Children's Hospital, New Hyde Park, NY

The Role of C5a in the Mobilization of Hematopoietic Stem/Progenitor Cells

Ali Jalili, MSc, PhD1*, Neeta Shirvaikar, MSc2*, Chris Korol1* and Anna Janowska-Wieczorek, MD, PhD3

1Medicine, University of Alberta, Edmonton, AB, Canada
2Research and Development, Canadian Blood Services, Edmonton, AB, Canada
3Div. Clinical Hematology, Dept. of Medicine, University of Alberta, Edmonton, AB, Canada

Possible Epigenetic Regulation of Functionally Opposing Groups of Genes Involved in Ex Vivo Expansion of Cord Blood Stem Cells

Sudhakar Baluchamy, PhD*, Benjamin Petro, BS*, Hiroto Araki, MD, PhD, Valerie Lindgren, PhD* and Nadim Mahmud, MD, PhD

Hematology/Oncology, Dept. of Medicine, University of Illinois at Chicago, Chicago, IL

Measurement of Stem Cell Potency, Assessment of Acceptance Limits for Release Criteria and Prediction of Umbilical Cord Blood Engraftment Using a Replacement for the Colony-Forming Cell Assay

Ivan N. Rich, PhD1, Karen Hall, MT, (ASCP)1*, Holli Harper, MS1* and Brian M Freed, PhD, DABHI2*

1HemoGenix, Inc, Colorado Springs, CO
2University of Colorado Cord Blood Bank and ClinImmune Laboratories, Denver, CO

Presence of Soluble CD26 Peptidase Activity in Human Serum Albumin (FlexBumin): Implications for Cell Freezing and Thawing Protocols during Transplantation

Laura A. Paganessi*, Andrew L. Walker* and Kent W. Christopherson II, PhD

Section of Hematology & Stem Cell Transplantation, Rush University Medical Center, Chicago, IL

Characterization of Human CD34+ Hematopoietic Stem Cells Following Administration of G-CSF or Plerixafor

Michael P. Rettig, PhD*, William D. Shannon, PhD*, Julie Ritchey*, Matthew Holt*, Kyle McFarland*, Sandra Lopez*, Jeremy Gabriel* and John F. DiPersio, MD, PhD

Department of Medicine, Siteman Cancer Center, Washington University School of Medicine, Saint Louis, MO

Regulatory T Cells (Tregs) Can Be Isolated from G-CSF Mobilized PBSC after Monocyte Depletion and Inhibit Anti-Stem Cell T Cell Alloreactivity

Dolores Mahmud, PhD* and Damiano Rondelli, MD

Section of Hematology/Oncology, University of Illinois at Chicago, Chicago, IL

*signifies non-member of ASH