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Hematopoiesis - Microenvironment, Cell Adhesion, and Mesenchymal Stem Cells Poster II

Sunday, December 7, 2008: 6:00 PM-8:00 PM
Hall A (Moscone Center)
Combined Action of Endothelial and Mesenchymal Niche Cells to Amplify Hematopoietic Progenitor Expansion in a Humanized System

Margareta Frühwirth1*, Daniela Thaler1*, Andreas Reinisch1*, Katharina Schallmoser2*, Eva Rohde2*, Nicole A Hofmann3*, Werner Linkesch4* and Dirk Strunk1

1Department of Hematology and Stem Cell Transplantation, Stem Cell Research Unit Graz, Medical University of Graz, Graz, Austria
2University Clinic of Blood Group Serology and Transfusion Medicine, Stem Cell Research Unit Graz, Medical University of Graz, Graz, Austria
3Stem Cell Research Unit Graz, Medical University of Graz, Graz, Austria
4Department of Hematology & Stem Cell Transplantation, Medical University of Graz, Graz, Austria

Adipocyte-Derived Stromal Cells Facilitate Hematopoiesis in Vitro and in Vivo: Advantages Over Bone Marrow-Derived Stromal Cells

Norihiko Nakao1*, Takayuki Nakayama, MD, PhD1*, Koji Yamamoto, MD, PhD2* and Tomoki Naoe, MD, PhD1

1Nagoya University Graduate School of Medicine, Department of Hematology and Oncology, Nagoya, Japan
2Nagoya University Hospital, Department of Transfusion Medicine, Nagoya, Japan

MicroRNA Mir-23a Regulates SDF-1alpha Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells

Fernando Fierro1*, David Poitz2*, Jan A. Nolta, PhD3, Martin Bornhaeuser4*, Gerhard Ehninger4 and Thomas Illmer5

1Hematology, Medical Clinic I - Technical University Dresden, Dresden, Germany
2Cardiology, Medical Clinic II - Technical University Dresden, Dresden
3University of California at Davis, Sacramento, CA
4Medical Clinic I, University Hospital, Dresden, Germany
5Medizinische Klinik I, University Hospital Carl Gustav Carus, Dresden University of Technology, Dresden, Germany

Mesenchymal Stem Cells Ameliorate Murine Progeria Phenotype

Jakub Tolar, MD, PhD1, Robert C Pueringer, B.S.1*, Ron T McElmurry, B.S.1*, Huber R Warner, Ph.D.2*, Stephen G Young, M.D.3* and Bruce R Blazar, M.D.4

1Univ. of Minnesota, Minneapolis, MN
2College of Biological Sciences, Univ. of Minnesota, Minneapolis, MN
3Department of Medicine, University of California, Los Angeles, Los Angeles, CA
4Pediatric Heme/Onc/BMT, University of Minnesota, Minneapolis, MN

Human Mesenchymal Stem Cells Exert Immunosuppressive Capacities by Induction of Regulatory T Cells

Kirsten Canté-Barrett, PhD*, Jessica M.E. van den Oever* and Willem E. Fibbe, MD, PhD

Dep. of Immunohematology and Bloodtransfusion, Leiden University Medical Center, Leiden, Netherlands

Disruption of Leukemia/Stroma Cell Interactions by CXCR4 Antagonist CTCE-9908 Enhances Chemotherapy-Induced Apoptosis in AML

Hongbo Lu1*, Zhihong Zeng, PhD2*, Yuexi Shi2*, Sergej Konoplev, MD, PhD1, Donald Wong, PhD3*, Marina Konopleva, MD, PhD4 and Michael Andreeff, MD, PhD2

1Hematopathology, The University of Texas M.D. Anderson Cancer Center, Houston, TX
2Stem Cell Transplantation and Cellular Therapy, The University of Texas M.D. Anderson Cancer Center, Houston, TX
3Chemokine Therapeutics Corp., Vancouver, BC, Canada
4Leukemia, The University of Texas M.D. Anderson Cancer Center, Houston, TX

Modulation of Mesenchymal Stem Cell Immunogenicity through Forced Expression of Human Cytomegalovirus Proteins

Melisa Soland, BS*, Mariana Bego, PhD*, Christopher D Porada, PhD*, Esmail D. Zanjani, PhD*, Stephen St Jeor, PhD* and Graca Almeida-Porada, MD, PhD

Animal Biotechnology, University of Nevada, Reno, Reno, NV

Mesenchymal Stromal Cells Promote a Sustained Increase of the CD69 Marker on Activated CD3+ Lymphocytes: Potential Immunomodulatory Role

Felipe Saldanha Araujo1*, Rodrigo Alexandre Panepucci1*, Kelen Cristina Farias1*, Amelia G Araujo2*, Maristela Delgado Orellana1*, Camila Branquinho Menezes1*, Patricia bonini Palma1*, Dimas Tadeu Covas1* and Marco Antonio Zago2*

1Internal Medicine, São Paulo University, Ribeirão Preto, Brazil
2Internal Medicine, Sao Paulo University, Ribeirao Preto, Brazil

Intravital Imaging of Young and Aged Stem Cells in the Marrow of Long Bones: Visualizing Mammalian Stem Cell Behavior in Real-Time in Vivo

Anja Köhler1*, Vince Schmithorst2*, Marie-Dominique Filippi, PhD3, Marnie A. Ryan, PhD4*, Deidre Daria4*, Matthias Gunzer1* and Hartmut Geiger, PhD5*

1Institute of Molecular and Clinical Immunology, Otto von Guericke University, Magdeburg, Germany, Magdeburg, Germany
2Imaging Research Center, Cincinnati Children's Hospital Medical Center, Cincinnati, OH
3Cincinnati Children's Hosp. Med. Ctr., Cincinnati, OH
4Division of Experimental hematology and cancer biology, Children's Hospital Medical Center, Cincinnati, OH
5Division of Experimental hematology and cancer biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH

Granulocyte Colony-Stimulating Factor (G-CSF) Stimulates Sympathetic Nervous System Activity in the Bone Marrow

Michela Battista, PhD1*, Simon Mendez-Ferrer, PhD2* and Paul S. Frenette, M.D.3

1Medicine, Mount Sinai School of Medicine, New York, NY
2Med. Div. of Hem., Mount Sinai School of Med., New York, NY
3Department of Medicine and Immunology Institute, Mount Sinai School of Medicine, New York, NY

Microenvironment Determines Myeloid-Erythroid Lineage Switch in MN1 Leukemia

Michael Heuser1*, Geyongsin Park1*, Ping Xiang1*, Sarah Vollett1*, Malina Leung1*, Courteney Lai1*, Grace Lin1* and R. Keith Humphries, MD, PhD2

1British Columbia Cancer Agency, Vancouver, BC, Canada
2Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada

Hematopoietic Stem Cells in the Mouse Spleen

Akiko Iseki*, Yohei Morita*, Hiromitsu Nakauchi* and Hideo Ema

Division of Stem Cell Therapy, Institute of Medical Science, University of Tokyo, Tokyo, Japan

Human Primary Mensenchymal Stromal Progenitor Cells Are Highly Enriched in Both, the CD271+/CD146+ and CD271+/CD146- Bone Marrow Population with the Latter Acquiring CD146 Expression upon Culture in-Vitro

Ariane Tormin, MS1*, Jan Claas Brune1*, Stuart Walsh, BS1*, Johan Richter, MD, PhD2*, Xiaolong Fan, MD, PhD3 and Stefan Scheding, MD1

1Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, University of Lund, Lund, Sweden
2Department of Molecular Medicine, University of Lund, Lund, Sweden
3The Rausing Laboratory, Lund University, Lund, Sweden

Expression and Function of the CXCR7 Chemokine Receptor in Leukemias

Sergej Konoplev, MD, PhD1, Hongbo Lu, MMSC1*, Michael A Fiegl, MD2*, Zhihong Zeng, PhD2*, Wenjing Chen, MD2*, Michael Andreeff, MD, PhD2 and Marina Konopleva, MD, PhD3

1Hematopathology, The University of Texas M.D. Anderson Cancer Center, Houston, TX
2Stem Cell Transplantation and Cellular Therapy, The University of Texas M.D. Anderson Cancer Center, Houston, TX
3Leukemia, The University of Texas M.D. Anderson Cancer Center, Houston, TX

Identification and Functional Analysis of Novel miRNA Target Genes That Control Mesenchymal Stem Cell Function

Maria Roubelakis, PhD1*, Dimitra Zagoura1*, Ourania Trohatou1*, Pantelis Zotos2*, Vassiliki Bitsika1*, Sophia Kossida, PhD2*, Kalliopi I Pappa, MD, PhD3*, Aristidis Antsaklis, MD, PhD3* and Nicholas P. Anagnou, MD, PhD4

1Cell and Gene Therapy Laboratory, Biomedical Research Foundation of the Academy of Athnes, Athens, Greece
2Bioinformatics Department, Biomedical Research Foundation of the Academy of Athnes, Athens, Greece
3Department of Obstetrics and Gynecology, University of Athens School of Medicine, Athens, Greece
4Laboratory of Biology, University of Athens, Athens, Greece

Lymphocyte-Activating Chemokines Are Potent Inducers of Sudden Arrest and Firm Adhesion in Mesenchymal Stromal Cells (MSCs) Under Shear Flow

Felicia Ciuculescu1*, Melanie Giesen1*, Erika Deak1*, Erhard Seifried, MD, PhD2, Tanja Nicole Hartmann3* and Reinhard Henschler4

1University of Frankfurt, Institute for Transfusion Medicine and Immune Hematology, Frankfurt, Germany
2University of Frankfurt, Institute of Transfusion Medicine and Immune Hematology, Frankfurt, Germany
3Third Medical Department, Laboratory of Immunological and Molecular Cancer Research (LIMCR), University Hospital, Salzburg, Austria, Salzburg, Azerbaijan
4Institute for Transfusion Medicine and Immunohaematology, Blood Transfusion Centre of the German Red Cross, Frankfurt/Main, Germany

The Small GTPase Rap1A Is Central for Migration and Adhesion of Mesenchymal Stromal Cells (MSCs)

Erika Deak1*, Brigitte Ruester2*, Stephan Göttig2*, Elena Puccetti, Ph.D.3*, Martin Ruthardt, MD4*, Erhard Seifried, MD, PhD5, Jürgen Scheele, MD6* and Reinhard Henschler7

1Production, Institute for Transfusion Medicine and Immune Hematology, Frankfurt, Germany
2University of Frankfurt, Institute of Transfusion Medicine and Immune Hematology, Frankfurt
3Institute of Molecular Biology, Marburg, Germany
4Department of Internal Medicine II, Haematology, Goethe-University of Frankfurt, Frankfurt/Main, Germany
5University of Frankfurt, Institute of Transfusion Medicine and Immune Hematology, Frankfurt am Main, Germany
6Internal Medicine I, University of Freiburg, Freiburg
7Institute for Transfusion Medicine and Immunohaematology, Blood Transfusion Centre of the German Red Cross, Frankfurt/Main, Germany

Id1 Provides a Proper Hematopoietic Progenitor Niche Function

Hyung Chan Suh, MD, PhD, Ming Ji*, John Gooya*, Michael Lee*, Kimberly Klarmann* and Jonathan R. Keller*

Laboratory of Cancer Prevention, National Cancer Inst. - Frederick, Frederick, MD

Clonal Abnormalities in MSC Derived from AML Bone Marrows

Michael Andreeff, MD, PhD1, Teresa McQueen, BS1*, Christopher Williams2*, Jun Gu, MS, MD3*, Vicki L. Hopwood, MS, BS3*, Taylor Appleberry2*, Corinn Rich2*, Stephanie Dallaire, PhD, MD2*, Steven Kornblau, MD1* and Rui-Yu Wang, MD, PhD1

1Stem Cell Transplantation and Cellular Therapy, The University of Texas M.D. Anderson Cancer Center, Houston, TX
2PerkinElmer, Inc., Waltham, MA
3School of Health Sciences, The University of Texas M.D. Anderson Cancer Center, Houston, TX

Acute Lymphoblastic Leukemia Blast Cells Stimulate the Autocrine Production of CCL2 and IL-8 in Bone Marrow Stromal Cells

Jaira Ferreira de Vasconcellos, BSc, MD1*, Nilson Ivo Tonin Zanchin, PhD2*, Angelo A. Cardoso, MD., PhD3, Silvia Regina Brandalise, MD, PhD4* and José Andrés Yunes, PhD5*

1Medical Genetics, Centro Infantil Boldrini, State University of Campinas, Campinas / SP, Brazil
2Molecular Biology Laboratory, Laboratório Nacional de Luz Síncrotron, Campinas, SP, Brazil
3Cancer Research Institute, Indiana University School of Medicine, Indianapolis, IN
4Pediatric Hematology/Oncology, Centro Infantil Boldrini, Campinas, Brazil
5Molecular Biology Laboratory, Centro Infantil Boldrini, Campinas, SP, Brazil

*signifies non-member of ASH