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Hematopoietic Stem and Progenitor Biology Poster II

Sunday, December 7, 2008: 6:00 PM-8:00 PM
Hall A (Moscone Center)
Thrombopoietin Administration Alleviates Hematopoietic Stem Cells Intrinsic Long Term Ionizing Radiation Damages. Identification of Preferential Cellular Expansion SitesClinically Relevant Abstract

Diana Tronik-Le Roux1*, Johnny Nehme1*, Arthur Simonnet1*, Pierre Vaigot1*, Marie Anne Nicola2*, Paquita Nurden3 and Philippe Leboulch4*

1Institute of Emerging Diseases and Innovative Therapies, Atomic Energy Commission (CEA), Evry, France
2Plate-forme d'Imagerie Dynamique, Pasteur Institute, Paris, France
3Centre de Référence des Pathologies Plaquettaires, Pessac, France
4Institut des Maladies Emergentes et des Thérapies Innovantes, CEA, Fontenay aux Roses, France

Human Embryonic Stem Cells-Derived Hemangioblasts Express HLA Antigens and Genes Related to Hematopoeisis and Endopoiesis

Grzegorz Wladyslaw Basak, MD, PhD1*, Satoshi Yasukawa, MD2*, Boris Minev, MD3* and Ewa Carrier, MD2*

1Department of Hematology/Oncology, Medical University of Warsaw, Warsaw, Poland
2Medicine, University of California San Diego, La Jolla, CA
3Neurosurgery, University of California San Diego, La Jolla, CA

Tumor Suppressor Protein p53 Affects Megakaryocytic Maturation: In Vivo and Ex Vivo Post-Translational Modification Studies

Pani A. Apostolidis1*, Anne Duchoud2*, Stephan Lindsey, PhD3*, William M. Miller, PhD4 and Eleftherios Papoutsakis, PhD3

1Northwestern University and Delaware Biotechnology Institute, Newark, DE
2Ecole Polytechnique Federale de Lausanne and Delaware Biotechnology Institute, Newark, DE
3University OF Delaware, Newark, DE
4Northwestern University, Evanston, IL

CD45-/ALDHlow/SSEA-4+/Oct-4+/CD133+/CXCR4+/Lin- Very Small Embryonic-Like (VSEL) Stem Cells Isolated from Umbilical Cord Blood as Potential Long Term Repopulating Hematopoietic Stem Cells

Ewa K Zuba-Surma, Ph.D.*, Magdalena Kucia, Ph.D., Rui Liu, M.D.*, Mariusz Z Ratajczak, M.D., Ph.D. and Janina Ratajczak, M.D., Ph.D.

James Graham Brown Cancer Center, Stem Cell Institute University of Louisville, Louisville, KY

Nicotinamide Increases the Megakaryocytic Maturation of Human Hematopoietic Stem Cells Primarily Due to SIRT Inhibition

Swapna Panuganti1*, Lisa M. Giammona, PhD1*, Jan M. Kemper1*, Pani Apostolidis2*, Stephan Lindsey, PhD2*, Eleftherios Papoutsakis, PhD2 and William M. Miller, PhD1

1Northwestern University, Evanston, IL
2University OF Delaware, Newark, DE

A Practical Erythroid Assay in Humanized Xenograft Mouse Model

Jun Hayakawa, MD, PhD1, Matthew Hsieh, MD1, Naoya Uchida, MD1, Kareem Washington, PhD1*, Oswald Phang1*, David Eric Anderson, PhD2* and John Tisdale, MD1*

1MCHB, NHLBI, NIH, Bethesda, MD
2Proteomics and Mass Spectrometry Facility, OD,NIDDK,NIH, Bethesda, MD

Disruption of the Osteoblast Niche by G-CSF Is Associated with Hematopoietic Stem Cell Quiescence and Loss of Long-Term Repopulating Activity: Role of Cdkn1a

Priya K. Gopalan, MD, PhD, Matthew J. Christopher, PhD*, Adam M. Greenbaum* and Daniel C. Link, MD

Internal Medicine, Washington University School of Medicine, Saint Louis, MO

Notch-Dependent Control of Blood Lineage Development is Modified by Fucosylation

Lan Zhou, MD, PhD1*, Quanjian Yan1*, David Yao1*, Lebing W Li1*, Stanton L. Gerson, MD2* and John B Lowe, MD3*

1Pathology, Case Western Reserve University, Cleveland, OH
2Department of Internal Medicine, University Hospitals of Cleveland, Case Medical Center, Cleveland, OH
3Pathology, Genentech, Inc, South San Francisco, CA

Probing the Mitotic History and Developmental Stage of Hematopoietic Cells Using Single Telomere Length Analysis (STELA)

Mark Hills1*, Kai Lucke1*, Connie J. Eaves, PhD1 and Peter M. Lansdorp, MD, PhD2

1Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
2BC Cancer Research Centre, Terry Fox Laboratory, Vancouver, BC, Canada

Erythropoietin Stimulates Thrombopoiesis in the Absence of C-Mpl Signaling

Norma E Fox, BSc1, Rose Chen, BSc1*, Ian Hitchcock, PhD2, Kenneth Kaushansky, MD3 and Amy Geddis, MD, PhD4

1School of Medicine, University of California, San Diego, La Jolla, CA
2School of Medicine, UCSD, La Jolla, CA
3Chair, Dept. of Medicine, President, American Society of Hematology, University of California - San Diego, La Jolla, CA
4School of Medicine, University of California San Diego, San Diego, CA

IL7 Counteraction with Notch Signaling Followed by EBF1 Expression Marks the B-Cell Commitment in CLP Stage

Sasan Zandi, MD*, Panagiotis Tsapogas, PhD*, Robert Månsson, PhD* and Mikael Sigvardsson, Professor*

Medicine, Molecular and Exprimental Medicine, Linkoping, Sweden

The HaemAtlas: Characterising Gene Expression in Differentiated Human Blood Cells

Nicholas A. Watkins1*, Marloes R. Tijssen1*, Arief Gusnanto2*, Bernard de Bono3*, Subhajyoti De4*, Diego Miranda-Saavedra5*, Will Angenent6*, Anthony Attwood1*, Cordelia Langford6*, Wendy N. Erber1, Nicola Foad1*, Stephen F Garner1*, Augusto Rendon1*, Catherine Rice6*, Daphne Thijssen-Timmer7*, C. Ellen Van der Schoot7*, Frank Dudbridge2*, Sarah Teichmann4*, Bertie Gottgens5* and Willem H. Ouwehand1*

1Dept. of Haematology, University of Cambridge & NHS Blood and Transplant, Cambridge, United Kingdom
2Biostatistics Unit, MRC, Cambridge, United Kingdom
3European Bioinformatics Institute, Hinxton, United Kingdom
4Laboratory of Molecular Biology, MRC, Cambridge, United Kingdom
5Dept. of Haematology, University of Cambridge, Cambridge, United Kingdom
6Wellcome Trust Sanger Institute, Hinxton, United Kingdom
7Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands

In Vivo Cellular Imaging Pinpoints the Role of Reactive Oxygen Species in the Early Steps of Adult Hematopoietic Reconstitution

Daniel Lewandowski1*, Vilma Barroca1*, Frédéric Ducongé2*, Bertrand Tavitian2* and Paul-Henri Roméo1*

1Dsv/Lrts, CEA/iRCM, Fontenay aux roses, France
2Dsv/Lime, CEA/I2BM, Orsay, France

Extensively Self-Renewing Erythroid Precursors Emerge from the Embryo but Not from the Adult

Samantha England, MS1*, Kathleen E. McGrath, PhD1* and James Palis, MD2

1Center for Pediatric Biomedical Research, University of Rochester, Rochester, NY
2Pediatrics, University of Rochester, Rochester, NY

Inhibition of CD26/Dipeptidylpeptidase IV Enhances Proliferation of Hematopoietic Progenitor Cells Induced by Selected Colony Stimulating Factors

Hal E. Broxmeyer, PhD, Scott Cooper*, Giao Hangoc, DVM and Timothy B. Campbell*

Indiana University School of Medicine, Walther Oncology Center, Indianapolis, IN

Notch1 Expression Is Regulated at Post-Transcriptional Level by 3'UTR Region in Hematopoietic Stem Cell Development

Shin-ichi Mizuno, MD, PhD*, Hidetoshi Ozawa, PhD*, Tadafumi Iino, MD*, Yojiro Arinobu, MD, PhD*, Chong Yong, MD, PhD*, Hirokazu Shigematsu, MD, PhD* and Koichi Akashi, MD, PhD

Dana-Farber Cancer Institute, Cancer Immunology and AIDS, Boston, MA

Lineage Instructive Function of  C/EBPα  in Multipotent Hematopoietic Progenitor Cells Revealed in a Novel Cebpa-Cre Knock-in Model

Albert Wolfler*, Astrid A Danen-van Oorschot*, Jurgen Haanstra*, Marijke Valkhof*, Paulette van Strien*, Elwin Rombouts* and Ivo P Touw

Department of Hematology, Erasmus Medical Center, Rotterdam, Netherlands

A Functional Screen to Identify Novel Effectors of Hematopoietic Stem Cell Activity

Eric Deneault1*, Sonia Cellot, MD2*, Amélie Faubert2*, Jean-Philippe Laverdure2*, Mélanie Fréchette2*, Jalila Chagraoui, PhD3 and Guy Sauvageau, MD, PhD4

1Iric, University of Montreal, Montreal, QC, Canada
2Molecular Genetics of Stem Cells, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, QC, Canada
3University of Montreal, Montreal, QC, Canada
4Institute for Research in Immunology and Cancer, University of Montreal, Montreal, QC, Canada

New Candidate Regulators of Hematopoietic Stem Cells Identified from Transcriptional Comparisons of Highly Purified Populations of Longterm Repopulating Cells with Markedly Different in Vivo Self-Renewal Activities

David G Kent, BSc1*, Michael R Copley, B.Sc.1*, Claudia Benz, PhD1*, Brad J Dykstra, PhD1*, Elaine Ma, BSc1*, Jay Cheyne, BSc1*, Yongjun Zhao, MSc1*, Maura Gasparetto, MD1*, Allen Delaney, PhD2*, Clayton Smith, MD, FRCPC3, Marco Marra, PhD4 and Connie J. Eaves, PhD1

1Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
2Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada
3Leukemia/BMT Program of British Columbia, Division of Hematology, Vancouver, BC, Canada
4Genome Sciences Center, British Columbia Cancer Agency, Vancouver, BC, Canada

Ultraconserved Genomic Regions (UCRs) Expression in Hematopoiesis

Ramiro Garzon, MD1, Catherine E.A Heaphy, MS1*, Chang Gong Liu, PhD2*, George A. Calin, MD, PhD2* and Carlo M. Croce, MD1*

1The Ohio State University, Columbus, OH
2Experimental Therapeutics and Cancer Genetics, University of Texas, MD Anderson Cancer Center, Houston, TX

An RNA Interference Screen Reveals Fate Determinants Implicated in Asymmetric Cell Division as Potential Regulators of Hematopoietic Stem Cell Self-Renewal

Kristin J Hope, Ph.D.*, Sonia Cellot, MD*, Stephen Ting, MD, PhD and Guy Sauvageau, MD, PhD

Institute for Research in Immunology and Cancer, University of Montreal, Montreal, QC, Canada

Efficient Production of Human Hematopoietic Progenitors from Human Pluripotent Stem Cells Using Chemically Defined Media without Serum or Feeder Cells

Zhaohui Ye*, Xiaobing Yu and Linzhao Cheng*

Institute for Cell Engineering, Johns Hopkins University, Baltimore, MD

The Side Population Contains Functionally Distinct Hematopoietic Stem Cell Subpopulations

Grant Anthony Challen, PhD1* and Margaret A Goodell, PhD2

1Cell And Gene Therapy, Baylor College of Medicine, Houston, TX
2Pediatrics, Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX

The Dual Bromodomain Protein Brd2 Controls Primary B Cell Mitogenesis and Cell Cycle in Mice Reconstituted with Lentivirus-Transduced HSCs

Wanda P. Blanton, MD1*, Fangnian Wang, MD, PhD2*, Hongsheng Liu, DMD2*, Paul Romesser2*, Douglas Faller3 and Gerald Denis, MSc, PhD4

1Immunology Training Program and Section of Gastroenterology, Boston University School of Medicine, Boston, MA
2Cancer Research Center and Section of Hematology/Oncology, Boston University School of Medicine, Boston, MA
3Boston Univ. School of Med., Boston, MA
4Boston Univ. Sch. of Med., Boston, MA

Myeloid Translocation Gene 16 (MTG16) Regulates Lymphopoiesis

Aubrey A Hunt1*, Melissa Ann Steapleton1*, Isabel Moreno, PhD2* and Scott Hiebert, PhD3*

1Vanderbilt University Cancer Center, Nashville, TN
2Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC
3Vanderbilt Cancer Center, Nashville, TN

System-Level Analysis of Two Erythroid Progenitor Survival Pathways Reveals Their Distinct Dynamical Properties

Miroslav Koulnis*, Alberto Porpiglia*, Ying Liu, MD* and Merav Socolovsky, MBBS, PhD

Department of Pediatrics and Department of Cancer Biology, the University of Massachusetts Medical School, Worcester, MA

Comprehensive Profiling of Micrornas in Murine Hematopoietic Stem Cells and Lineages Using a Microfluidics Approach

Florian Kuchenbauer, MD1*, Oleh I Petriv, PhD2*, Allen Delaney3*, David G Kent, BSc4*, Michael Heuser5*, Sarah M Mah4*, Michael R Copley, B.Sc.6*, Jens Rüschmann1*, Frann Antignano6*, Etushi Kuroda1*, Victor Ho1*, Claudia Benz, PhD6*, Timotheus You Fu Halim7*, Vincenzo Giambra1*, Gerald Krystal, PhD8, Connie J. Eaves, PhD6, Fumio Takei, PhD7*, Andrew P. Weng, MD, PhD5, Marco Marra, PhD9, Carl L Hansen, PhD2* and R. Keith Humphries, MD, PhD6

1Terry Fox Laboratory, BC Cancer Research Centre, Vancouver, BC, Canada
2Center for High-Throughput Biology, Department of Physics and Astronomy, Michael Smith Laboratories
3Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada
4Terry Fox Lab, BCCRC, Vancouver, BC, Canada
5British Columbia Cancer Agency, Vancouver, BC, Canada
6Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
7Terry Fox Laboratories, BC Cancer Research Centre, Vancouver, BC, Canada
8British Columbia Cancer Research Centre, Vancouver, BC, Canada
9Genome Sciences Center, British Columbia Cancer Agency, Vancouver, BC, Canada

Graded Repression of PU.1 Expression by GATA Factors Influence Hematopoietic Progenitor Cell Fate

Stella T. Chou, MD1, Charles Bailey, M.D.2*, Yu Yao3*, Zan Huang, PhD4*, Kim Nichols, MD2*, John Crispino, PhD5, Ross Hardison, Ph.D.6*, Gerd Blobel, MD, PhD3*, Chris Vakoc, MD, PhD3* and Mitchell J. Weiss, M.D., Ph.D.3

1Div of Hematology/Dept of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA
2Div of Oncology/Dept of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA
3Division of Hematology, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA
4Dept Med/Div Hematol&Oncol, Northwestern University, Chicago, IL
5Medicine, Hematology/Oncology, Northwestern University, Chicago, IL
6Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA

*signifies non-member of ASH