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711.P1.32 Ex Vivo Expansion, Mobilization and Engraftment Poster I

Sunday, December 7, 2008: 6:00 PM-8:00 PM
Hall A (Moscone Center)
2301
Comparison of High-Dose Cyclophosphamide and Growth Factor with Growth Factor Alone for Mobilization of Stem Cells for Transplantation in Patients with Multiple Myeloma

Morie Abraham Gertz, MD1, Shaji Kumar1*, Martha Lacy1*, Angela Dispenzieri1*, Suzanne Hayman1*, Francis Buadi1*, David Dingli1*, Dennis Gastineau1*, Jeffery Winters2* and Mark Litzow1*

1Division of Hematology, Mayo Clinic, Rochester, MN
2Division of transfusion medicine, Mayo Clinic, Rochester, MN
2302
Standard Versus High Dose Lenograstim in Adults with Hematological Malignancies for Peripheral Blood Progenitor Cell Mobilization: Results of a Retrospective Study on Behalf of Rome Transplant Network

Azzurra Anna Romeo1*, Anna Chierichini2*, Diana Giannarelli3*, Alessandra Spagnoli3*, Barbara Anaclerico2*, Mariangela Vittori4*, Michele Vacca5*, Maria Gozzer6*, Antonio Spadea, MD, PhD7, Luciana Annino2*, Maria Concetta Petti8*, Andrea Mengarelli8* and William Arcese, MD9

1Hematology Unit, Campus Biomedico University, Rome, Italy
2Hematology Unit, S.Giovanni-Addolorata Hospital, Rome, Italy
3Biostatistical Unit, Regina Elena Cancer Institute, Rome, Italy
4SC Immuno-Transfusional Medicine, S.Giovanni-Addolorata Hospital, Rome, Italy
5UOS Centro Raccolta HPC-A SIMT, S.Camillo-Forlanini Hospital, Rome, Italy
6Transfusional Medicine Department, La Sapienza University, Rome, Italy
7Dept. of Clinical Oncology, Hematology-Istituto Regina Elena, Rome, Italy
8Hematology Unit, Regina Elena Cancer Institute
9Dept. of Hematology and Transplant, University of Rome 'Tor Vergata', Rome, Italy
2303
Human Erythroblasts Generated in Vitro Remain Functional with a Normal Karyotype 8 Years after Cryopreservation: Implications for Ex Vivo Generated Erythroid Transfusion Products

Massimo Sanchez, PhD1*, Amanda Leblanc2*, Annalisa Mancini, PhD3*, Francesca Masiello, PhD4*, Valentina Tirelli, PhD4*, Giovanni Migliaccio, PhD4*, Anna Rita Migliaccio, PhD5*, Vesna Najfeld, PhD6 and Carolyn Whitsett, MD7*

1Istituto Superiore di Sanita', Rome, Italy
2Pathology and Medicine, Mount Sinai School of Medicine, New York, NY
3Medicine, Mount Sinai School of Medicine, New York, NY
4Cell Biology and Neuroscience, Istituto Superiore di Sanita', Rome, Italy
5Hematology and Oncology, Mount Sinai School of Medicine, New York, NY
6Pathology and Medicine, The Mount Sinai School of Medicine, New York, NY
7Blood Bank, Mount Sinai Medical Center, New York, NY
2304
Influence of Bone Marrow Graft B Lymphocyte Subsets on Outcome after Transplantation from HLA-Identical Siblings

David Michonneau1*, Regis Peffault de Latour2*, Raphael Porcher3*, Marie Robin1*, Marc Benbunan, PhD4*, Vanderson Rocha5, Patricia Ribaud1*, Christelle Ferry1*, Agnes Devergie1*, Eliane Gluckman, MD6, Jean-Pierre Marolleau4*, Gerard Socie, MD, PhD7* and Jerome Larghero4*

1Hematology / Transplantation, Hospital Saint Louis, Paris, France
2Hematology / Transplantation, Hospital St Louis, Paris, France
3Inserm U 717, Hospital Saint Louis, Paris, France
4Cellular Therapy, Hospital Saint Louis, Paris, France
5BMT unit, Hopital Saint Louis APHP and Université Paris VII, Paris, France
6Hopital Saint-Louis, Paris, France
7Serv. de Greffe de Moelle, Paris, France
2305
Apoptosis-Independent Activation of Caspase-3 in CD34-Positive (CD34+) Mobilized Cells

Karine Augeul-Meunier1*, Carine Crampé2*, Philippe Farce2*, Christiane Mounier3*, Denis Guyotat1* and Lydia Campos, MD, PhD4

1Institut de Cancérologie de la Loire, Saint Etienne, France
2Laboratoire Hématologie, CHU Saint Etienne, Saint Etienne, France
3Haematology, CHU Saint Etienne, St Priest en Jarez, France
4Laboratoire Hematologie, Hopital Nord, Saint-Etienne, France
2306
Hematopoietic Colony Forming Unit: Development of a High-Throughput CFU Assay Strategy by the Use of High-Resolution Digital Images Stored in a Laboratory Information System

Maria S Albano, William Rothman*, Chiseko Watanabe*, Anna Gora*, Andromachi Scaradavou* and Pablo Rubinstein*

National Cord Blood Program, New York Blood Center, New York, NY
2307
Genomic Stability and Safety of MSCs after Animal Serum-Free Humanized Clinical Scale Propagation

Katharina Schallmoser1*, Eva Rohde1*, Andreas Reinisch1*, Anna C Obenauf2*, Christina Bartmann1*, Gerhard Lanzer3*, Werner Linkesch4* and Dirk Strunk1

1Stem Cell Research Unit Graz, Medical University of Graz, Graz, Austria
2Institute of Human Genetics, Medical University of Graz, Graz, Austria
3University Clinic for Blood Group Serology and Transfusion Medicine, Medical University of Graz, Graz, Austria
4Department of Hematology & Stem Cell Transplantation, Medical University of Graz, Graz, Austria
2308
Study of the Mechanisms by Which Angptl5 and IGFBP2 Support Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells

Junke Zheng1* and Chengcheng Zhang, PhD2

1UT Southwestern Medical Center, Dallas, TX
2UT Southwestern, Dallas, TX
2309
Megakaryocytic Potential of Unseparated Thawed Cord Blood Cells

Sari Möttönen*, Mikko Eskola*, Sari Juutistenaho* and Riitta Kekomäki

Finnish Red Cross Blood Service, Helsinki, Finland
2310
Autologous Peripheral Blood Stem Cell Products from Patients with Hematologic Malignancies Have Increased Frequency of Regulatory T Cells (CD4+FoxP3+ Treg)

Grace Kao, MD1, Kristen Stevenson2*, Elliot Kim1*, Elena Spanjaard, PhD1*, Haesook T Kim, PhD2* and Jerome Ritz, MD3

1Dana-Farber Cancer Inst., Boston, MA
2Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA
3Medical Oncology, Dana-Farber Cancer Institute, Boston, MA
2311
The Use of Clinical Grade Magnetic Beads to Deplete Immune Suppressive Elements from Autologous Peripheral Blood Hematopoietic Stem Cell Grafts Enhances T Cell Immune Responses

Zaid S Al-Kadhimi, M.D1*, Haider Mahdi, M.D2*, Moshrik Abdalamir, M.D1*, Elyse Paul1*, Voravit Ratanatharathorn, M.D1*, Joseph Uberti, M.D, Ph.D1* and Lawrence G. Lum, MD, DSC3

1Internal Medicine-Hematology/Oncology, Karmanos Cancer Center and Wayne State University, Detroit, MI
2Obstetrics/Gynecology, Wayne State University, Detroit, MI
3Barbara Ann Karmanos Cancer Institute, Detroit, MI
2312
PEG-Filgrastim for Autologous Peripheral CD34+ STEM CELL Collection in Pediatric Oncological PATIENTS: A PHASE II STUDY

Simone Cesaro1*, Gloria Tridello1*, Andrea Giulio Zanazzo2*, Stefano Frenos3*, Chiara Messina1* and Sandro Dallorso4*

1Department of Pediatrics, Pediatric Hematology Oncology, Padova, Italy
2Istituto Burlo Garofalo, Pediatric Hematology Oncology, Trieste
3Pediatric Hematology Oncology, Ospedale Meyer, Firenze
4Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
2313
Experimental Double Cord Blood Transplantation (DCBT) of An Expanded Unit Together with a Non-Manipulated Unit in NOD/SCID Mice

Tony Peled1*, Hadas Shoham1*, Oshrat Golan1*, Dorit Ashengrau1*, Dima Yaacobov1*, Gabi Frei1*, Arnon Nagler2, Eitan Fibach3 and Amnon Peled4*

1Gamida-Cell, Jerusalem, Israel
2Division of Hematology and Bone Marrow Transplantation, Chaim Sheba Medical Center, Tel Hashomer, Israel
3Department of Hematology, Hadassah - Hebrew University Medical Center, Jerusalem, Israel
4Goldyne Savad Institute of Gene Therapy, Hadassah - Hebrew University Medical Center, Jerusalem, Israel
2314
Impact of Rituximab on Peripheral Blood Stem Cell Mobilization and Collection Following ACVBP Regimen in Poor Risk Patients with Diffuse Large B-Cell Lymphoma (DLBCL): Results from a Large Cohort of Patients from Two Prospective GELA Trials

Francois Lefrere Sr., MD1*, Dominique bastit-Barrau, md2*, Suzanne Mathieu, md3*, Alain Bohbot, md4*, Philippe Bourrin, md5*, Gilles Andre Salles, MD, PhD6, Herve Tilly, MD7, Nicolas Mounier, MD8*, Olivier Fitoussi, MD9* and Corinne Haioun10*

1Hematology, Necker Hospital, Paris, France
2Transfusion, Etablissement Francais Du Sang, Rouen, France
3Transfusion, Etablissement Francais Du Sang, Nancy, France
4Transfusion, Etablissement Francais Du Sang, Strasbourg, France
5Transfusion, Etablissement Francais Du Sang, Toulouse, France
6Hematologie, Centre Hospitalier Lyon-Sud, Pierre-Benite, France
7Centre Henri Becquerel, Rouen, France
8CHU Nice, Nice, France
9Hematology, Bordeaux, France
10Hopital Henri Mondor, Creteil, France
2315
The Effect of Physical Conditions on the Transportation of Peripheral Blood Progenitor (PBPC) Products

Jan Jansen, MD, PhD.1, Pamela L Nolan, MT(ASCP)2*, Margaret I Reeves, BS3*, Luke Paul Akard, MD4, James M. Thompson, DO5, Michael J Dugan, MD6 and Susan G Hanks, MS7*

1Indiana Blood and Marrow Transplantation, Beech Grove, IN
2Stem Cell Laboratory, St.Francis Hospital and health Centers, Beech Grove, IN
3Stem Cell Laboratory, St.Francis Hospital and Health Centers, Beech Grove, IN
4IBMT, Beech Grove, IN
5Indiana BMT, Beech Grove, IN
6Inidiana Blood and Marrow Transplantation,, Beech Grove, IN
7Stem Cell Laboratory, Indiana Blood and Marrow Transplantation, Beech Grove, IN
2316
Optimization of Isolation and Further Molecular and Functional Characterization of SSEA-4+/Oct-4+/CD133+/CXCR4+/LINneg/CD45neg Very Small Embryonic-Like (VSEL) Stem Cells Isolated from Umbilical Cord Blood

Ewa K Zuba-Surma, Ph.D.1*, Magdalena Kucia, Ph.D.1, Izabela Klich, Ph.D.1*, Nicholas Greco, PhD2*, Mary L Laughlin, M.D., Ph.D.3*, Philip Paul, Ph.D.3*, Mariusz Z Ratajczak, M.D., Ph.D.1 and Janina Ratajczak, M.D., Ph.D.1

1James Graham Brown Cancer Center, Stem Cell Institute University of Louisville, Louisville, KY
2Cord Blood Bank, Case Western University, West Farmington, OH
3Cord Blood Bank, Case Western University, Cleveland, OH
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