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507.P1.21 Hematopoiesis - Malignant Stem and Progenitor Cells Poster I

Saturday, December 6, 2008: 5:30 PM-7:30 PM
Hall A (Moscone Center)
The Bone Morphogenetic Protein (BMP)-4 Is Involved in the Regulation of Human Megakaryocytic Differentiation during Thrombopoietin Signaling

Franck E Nicolini, MD, PhD1, Sandrine Jeanpierre, BSc2*, Bastien Kaniewski, BSc2*, Charles Dumontet, MD, PhD3, Ruth Rimokh, PhD2*, Alain Puisieux, PharmD, PhD2* and Véronique Maguer-Satta, PhD2*

1Hematology Department, Hospital E. Herriot, Lyon, France
2Inserm U590, Centre Léon Bérard, LYON, France
3Laboratoire d'Hématologie, Hopital Edouard Herriot, Lyon, France

Phenotypic and Functional Characterization of CD34+/CD38-/CD123+ Leukemic Progenitor (Stem) Cells in AML: a Flow Cytometric Approach

Harald Herrmann, MD1*, Christian Baumgartner, MD1*, Wolfgang R Sperr, MD1*, Steve Holmes, PhD2* and Peter Valent, MD1

1Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria
2Domantis Limited, Cambridge, United Kingdom

Effect of Parthenolide on Stem Cells in Childhood Acute Lymphoblastic LeukaemiaClinically Relevant Abstract

Paraskevi Diamanti, PhD, BSc1*, Charlotte V Cox, BSc1* and Allison Blair, PhD, BSc2

1Bristol Institute for Transfusion Sciences, Bristol, United Kingdom
2University of Bristol, Bristol, United Kingdom

Transformation of E2A-Deficient Pluripotent Progenitors by BCR-ABL Generates Imatinib-Resistant Leukemic Stem Cells

Yosuke Minami, MD, PhD1, Scott A. Stuart1*, Tomokatsu Ikawa, PhD2*, Akihiro Abe, MD, PhD3, Tomoki Naoe, MD, PhD3*, Catriona H.M. Jamieson, MD, PhD1* and Jean Y.J. Wang, PhD1*

1Deapartment of Medicine and Moores Cancer Center, University of California San Diego, La Jolla, CA
2Laboratory for Lymphocyte Development, Riken Research Center for Allergy and Immunology, Yokohama, Japan
3Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan

Role of Different C/EBPα Mutations in AML Transformation

Oscar Quintana-Bustamante1*, S. Lan-Lan Smith2*, Jude Fitzgibbon3 and Dominique Bonnet, PhD4

1Hematopoietic Stem Cell Laboratory, Cancer Research UK, London, United Kingdom
2Hemato-Oncology Section, The Institute of Cancer Research, Surrey, United Kingdom
3Centre for Medical Oncology, Barts and the London School of Medicine and Dentistry, London, United Kingdom
4Cancer Research UK, London Cancer Institute, London, United Kingdom

The JAK2V617F Mutation in Non-MPD Hematopoiesis Occurs at a Low Frequency and in Differentiating Erythroid CellsClinically Relevant Abstract

Svetlana Krichevsky1*, Evgenia Prus, PhD1*, Julia Abramowitz1*, Aliza Treves1*, Ido Weinberg, MD2*, Ariel Borohovitz1*, Riki Perlman, PhD1*, Eitan Fibach, Ph.D1 and Dina Ben-Yehuda, MD1

1Division of Hematology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel
2Division of Internal Medicine, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

Increased Aldehyde Dehydrogenase Activity Indentifies a Subset of Human Leukemic Blasts with Stem Cell Characteristics and Correlates with Poor Prognosis

Dan Ran1*, Mario Schubert, MD1*, Larissa Pietsch1*, Isabel Taubert1*, Christian Wallenwein1*, Katrin Miesala1*, Kerstin Woerner1*, Patrick Wuchter, MD1*, Volker Eckstein, PhD1*, Tom Bruckner, PhD2* and Anthony Ho, MD1

1Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany
2Department of Medical Biometry, University of Heidelberg, Heidelberg, Germany

Adipocytes Secrete Factors That Cause Leukemia Drug Resistance

James W Behan1*, Jason P Yun1*, Marina P Proektor1*, Ehsan A Ehsanipour1*, Anna Butturini, MD2, Nora Heisterkamp, PhD3* and Steven D Mittelman, MD, PhD1*

1Division of Endocrinology, Childrens Hospital Los Angeles, Los Angeles, CA
2Hematology/Oncology, Children's Hospital Los Angeles, Los Angeles, CA
3Pathology, Childrens Hospital Los Angeles, Los Angeles, CA

Hematopoietic Stem Cells, but Not Progenitors, Are the Exclusive Target for STAT5-Induced Long-Term Self-Renewal

Szabolcs Fatrai*, Albertus T.J. Wierenga*, Edo Vellenga, Simon M. G. J. Daenen* and Jan J. Schuringa

Hematology, University Medical Center Groningen, Groningen, Netherlands

In Childhood ALL, Both Blasts with a CD20-/Low and a CD20High Immunophenotype Have the Ability to Transfer the Leukemia Onto Immune-Deficient NOD/Scid Y-/- MiceClinically Relevant Abstract

Kerrie Wilson, BSc, (Hons)1*, Klaus Rehe, MD1*, Simon Bomken, MD1*, Marian Case, MSc1*, Leonard Shultz, PhD2, Julie Irving, PhD1 and Josef Vormoor, MD, FRCPCH1

1Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
2The Jackson Laboratory, Bar Harbor, ME

Long-Term Expansion and Self Renewal Is Contained in the CD34+, but Not the CD34- Subfraction of Nucleophosmin Mutated Acute Myeloid Leukemia CellsClinically Relevant Abstract

Carolien Woolthuis1*, Lina Han1*, Djoke van Gosliga1*, Philip Kluin, MD, PhD2, Edo Vellenga, MD, PhD3, Jan J. Schuringa, PhD4 and Geert Huls, MD, PhD5

1Hematology, University Medical Center Groningen, Groningen, Netherlands
2Pathology and Laboratory Medicine, University Medical Centre Groningen, Groningen, Netherlands
3Hematology, University Medical Hospital Groningen, Groningen, Netherlands
4Hematology, University Medical Center - Groningen, Groningen, Netherlands
5Hematology, University Medical Center Groninen, Groningen, Netherlands

The Polycomb Gene BMI1 Collaborates with BCR-ABL in Leukemic Transformation of Human Cord Blood CD34+ Cells

Aleksandra Rizo1*, Sandra Olthof2*, Ronald van Os, PhD3*, Bert HJ Dontje3*, Edo Vellenga, MD, PhD2, Gerald Haan, PhD3* and Jan Jacob Schuringa, PhD2

1Departments of Stem Cell Biology and Hematology, University Medical Center Groningen, Groningen, Netherlands
2Hematology, University Medical Center Groningen, Groningen, Netherlands
3Stem Cell Biology, University Medical Center Groningen, Groningen, Netherlands

Runx1 Deficiency Produces Aberrant Progenitor Self-Renewal through Selective Compromise of Pu.1 Mediated Transactivation but Not Transrepression

Zhenbo Hu1*, Soledad Negrotto1*, Kristine Marie Baraoidan2*, Vinzon Ibanez2*, Aron Shama2*, Fariborz Mortazavi2*, Steven Ackerman2*, Shrihari Kadkol2*, Giuseppina Nucifora2* and Yogen Saunthararajah1

1Experimental Hematology and Hematopoiesis Section, Taussig Cancer Center, Cleveland Clinic Foundation, Cleveland, OH
2University of Illinois at Chicago, Chicago, IL

The Histone Deacetylase Inhibitor LAQ824 Maintains Normal Hematopoietic Progenitor cells  (HPC) Associated with Induction of Notch Target Genes and Does Not Eliminate Leukemic HPC in Vitro

Kerstin Schwarz1*, Oliver Ottmann1, Annette Romanski1*, Anja Vogel1*, Jeffrey W. Scott2*, Hubert Serve1* and Gesine Bug1*

1Goethe-University of Frankfurt, Medizinische Klinik II, Frankfurt/Main, Germany
2Novartis Oncology, Florham Park, NJ

Specific Detection of Aberrant and Normal Stem Cells in Acute Myeloid Leukemia Patients Opens the Way for Defining Highly Specific Targets for Stem Cell Therapy

Monique Terwijn, MSc*, Angèle Kelder*, Arjo P Rutten*, Sonja Zweegman, MD, PhD, Gert J Ossenkoppele, MD, PhD and Gerrit Jan Schuurhuis, PhD*

Department of Hematology, VU University Medical Center, Amsterdam, Netherlands

Leukemia Stem Cells (LSCs) Are Frequent in Childhood Precursor B Acute Lymphoblastic Leukemia (ALL)Clinically Relevant Abstract

Sebastien Morisot, MS1*, Alan S Wayne, MD2*, Osnat Bohana-Kashtan, PhD1*, Ian M Kaplan, BS1*, Richard Hildreth, MS1*, Patrick Brown, MD3, Maryalice Stetler-Stevenson, MD, PhD4 and Curt I Civin, MD1*

1Division of Immunology and Hematopoiesis, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD
2Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD
3Division of Pediatric Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD
4Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, MD

Heterogeneity of Acute Myeloid Leukemia at the Stem Cell Level

Michael Heuser1*, Laura Sly1*, Courteney Lai1*, Malina Leung1*, Grace Lin1*, Bob Argiropoulos2*, Florian Kuchenbauer, MD3*, Stephen Fung1*, Christy Brookes1*, Gerald Krystal, PhD4 and R. Keith Humphries, MD, PhD5

1British Columbia Cancer Agency, Vancouver, BC, Canada
2Terry Fox Laboratory, BC Cancer Research Agency, Vancouver, BC, Canada
3Terry Fox Laboratory, BC Cancer Research Centre, Vancouver, BC, Canada
4British Columbia Cancer Research Centre, Vancouver, BC, Canada
5Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada

Activation of AKT1 in Hematopoietic Stem Cells Causes a Myeloproliferative Disease in a Tet-Inducible Mouse ModelClinically Relevant Abstract

Christian Brandts, MD1*, Miriam Rode, PhD2*, Beate Lindtner, MA2*, Gabriele Koehler, MD3*, Steffen Koschmieder, MD4, Carsten Mueller-Tidow5*, Wolfgang E. Berdel6 and Hubert Serve7*

1Internal Medicine, University Hospital of Frankfurt, Frankfurt, Germany
2Hematology, University of Muenster, Muenster, Germany
3Pathology, University of Muenster, Muenster, Germany
4Dept. of Int. Med. A, Hem./Onc., University Hospital of Munster, Munster, Germany
5Dept. of Medicine (Hematology and Oncology), University of Münster, Münster, Germany
6Dept. of Medicine (Hematology and Oncology), University of Münster Universitätsklinik Münster, Münster, Germany
7Dept. of Internal Medicine, University Hospital of Frankfurt, Frankfurt, Germany

Identification of Leukemia Stem Cells Associated with AML1-ETO through Characterization of Oncogene-Induced Changes in Cell Surface Antigen Profiles on Hematopoietic Stem CellsClinically Relevant Abstract

Carmen S Ballestas, Pediatric, hematology, oncology1*, Hyung-Gyoon Kim2*, Claude Scott Swindle2* and Christopher Klug, PhD2

1School of Medicine, Division of Pediatric hematology-oncology . University of Alabama at Birmingham, Birmingham, AL
2The Univ. of AL at Birmingham, Birmingham, AL

JunB Limits Hematopoietic Stem Cell (HSC) Functions as a Protective Mechanism against Initiation of Myeloid Malignancy

Marianne T Santaguida, PhD*, Koen Schepers, PhD*, Bryan C King* and Emmanuelle Passegue, PhD

Institute for Regeneration Medicine, University of California San Francisco, San Francisco, CA

HTLV-1 Infection of Human Hematopoietic Stem Cells Induces a Novel T Cell Lymphoma in Humanized SCID Mice

Prabal Banerjee, PhD1, Lindsey Crawford2*, Michelle Sieburg2*, Patrick Green, PhD3*, Mark A Beilke, MD4*, William J Harrington Jr., MD5*, Michael D Lairmore, DVM, PhD3* and Gerold Feuer, PhD2

1Center for Humanized SCID Mice Models, Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY
2Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY
3Department of Veterinary Biosciences, The Ohio State University, Columbus, OH
4Department of Medicine, Medical College of Wisconsin, Milwaukee, WI
5Division of Hematology/Oncology, Sylvester Comprehensive Cancer Center, University of Miami School of Medicine, Miami, FL

*signifies non-member of ASH