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506.P1.44 Hematopoiesis - Microenvironment, Cell Adhesion, and Mesenchymal Stem Cells Poster I

Saturday, December 6, 2008: 5:30 PM-7:30 PM
Hall A (Moscone Center)
Mesenchymal Stromal Cells (MSC) Isolated from Human Osteosarcomas Show a High Progenitor Cell Frequency, Typical MSC Morphology, Surface Marker Profile, and Differentiation Capacity, and They Are Considerably Affected by Tyrosine Kinase Inhibitors in Vitro

Jan Claas Brune, MS1*, Ariane Tormin, MS1*, Ulrike Bauer, MS1*, Ulf Neumann, MD2*, Pehr Rissler, MD, PhD3*, Otte Brosjö, MD, PhD4*, Richard Löfvenberg, MD, PhD5*, Fredrik Vult von Steyern, MD, PhD6*, Fredrik Mertens, MD, PhD7*, Anders Rydholm, MD, PhD6* and Stefan Scheding, MD1

1Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, University of Lund, Lund, Sweden
2Department of General, Visceral, and Transplantation Surgery, University Hospital Charité, Berlin, Germany
3Department of Pathology, Lund University Hospital, Lund, Sweden
4Department of Orthopedics, Karolinska Hospital, Stockholm, Sweden
5Department of Orthopedics, Umeå University Hospital, Umeå, Sweden
6Department of Orthopedics, Lund University Hospital, Lund, Sweden
7Department of Clinical Genetics, Lund University Hospital, Lund, Sweden

Perivascular/Mesenchymal Stem Cells from Multiple Human Tissues Support Hematopoiesis in Vitro

Elisa Montelatici, PhD1*, Gabriella Andriolo, PhD1*, Mihaela Crisan, PhD2*, Rosaria Giordano, MD1, Paolo Rebulla, MD1, Bruno Péault, PhD2* and Lorenza Lazzari, PhD1

1Cell Factory, Department of Regenerative Medicine, Center of Transfusion Medicine, Cellular Therapy and Criobiology, Fondazione Ospedale Maggiore Policlinico, Milano, Italy
2Stem Cell Research Center, Departments of Pediatrics and Orthopaedic Surgery, Pittsburgh, PA

The Role of Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) in Hematopoiesis

Yi Shen1*, Valerie Barbier1*, Ingrid G Winkler, PhD1*, Jean Hendy2* and Jean-Pierre Levesque, PhD1

1Mater Medical Research Institute, South Brisbane, Australia
2Peter MacCallum Cancer Centre, Australia

Angiopoietin-Like 3 Deficient Bone Marrow Has Decreased Ability to Support Hematopoietic Stem Cells

Junke Zheng1*, HoangDinh Huynh2* and Chengcheng Zhang, PhD3

1UT Southwestern Medical Center, Dallas, TX
2Physiology and Developmental Biology, UT Southwestern Medical Center, Dallas, TX
3UT Southwestern, Dallas, TX

A Cell-Autonomous Defect of Bone Marrow Hematopoietic Stem/Progenitor Cells and Impaired Stromal Function in FA-a Patients

Xiaoling Zhang1*, Xun Shang1*, Fukun Guo1*, Kim Murphy1*, Michelle Kirby1*, Patrick Kelly2, Lilith Reeves3*, Franklin O Smith4*, David A. Williams5, Yi Zheng3* and Qishen Pang3

1Experimental Hematology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH
2Wyeth Pharmaceuticals, Cambridge, MA
3Experimental Hematology and Pediatrics, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, OH
4Hematology/Oncology and Pediatrics, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, OH
5Hematology/Oncology, Children's Hospital Boston, Leland Fikes, Boston, MA

The Wnt Inhibitor sFRP2 Enhances Mesenchymal Stem Cell Engraftment, Granulation Tissue Formation and Myocardial Repair

Maria Paula Alfaro1*, Matthew Pagni1*, Alicia Vincent1*, Michael F. Hill, PhD2*, Ethan Lee, MD, PhD3* and Pampee Young, MD, PhD4

1Pathology, Vanderbilt University, Nashville, TN
2Cardiovascular Medicine Division, Vanderbilt University, Nashville, TN
3Cell and Developmental Biology, Vanderbilt University, Nashville, TN
4Vanderbilt University, Nashville, TN

TLR4-Agonist Stimulated Human Multipotent Mesenchymal Stromal Cells Enhance Both Hematopoietic Progenitors and Myeloid Differentiation

Patrick Ziegler, PhD1*, Steffen Boettcher1*, Guido Garavaglia, MD2* and Markus G. Manz, MD1

1Institute for Research in Biomedicine, Bellinzona, Switzerland
2Department of Orthopedic Surgery, Ospedale San Giovanni, CH-6500 Bellinzona, Switzerland

Human Hematopoietic Stem Cells and Leukemic Cells Form Cadherin-Catenin Based Junctional Complexes with Mesenchymal Stromal Cells

Patrick Wuchter, M.D.*, Rainer Saffrich, Ph.D.*, Wolfgang Wagner, Ph.D., M.D.*, Frederik Wein, Ph.D.*, Mario Stephan Schubert, M.D.*, Volker Eckstein, Ph.D.* and Anthony D. Ho, M.D., Ph.D.*

Department of Medicine V, University of Heidelberg, Heidelberg, Germany

Stabilin-1 and Stabilin-2 Are Expressed in Bone Marrow Sinusoidal Endothelial Cells and Mediate Scavenging and Cell Adhesive Functions

Hong Qian1*, Sophie Johansson2*, Peter McCourt3*, Bård Smedsrød3*, Marja Ekblom4* and Staffan Johansson5*

1Hematopoietic Stem Cell Laboratory; Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Swed, Lund, Sweden
2Dept. of Medical Biochemistry and Microbiology, Uppsala University, Sweden, Uppsala, Sweden
3Dept. of Cell Biology and Histology, University of Tromsø, Norway
4Department of Hematology, Lund University Hospital, Sweden
5Dept. of Medical Biochemistry and Microbiology, Uppsala University, Sweden

Maturation and Terminal Differentiation of Embryonic Erythroid Cells in the Developing Fetal Liver

Margaret H. Baron, MD, PhD, Joan Isern, PhD*, Stuart T. Fraser, PhD* and Zhiyong He, PhD*

Mount Sinai School of Medicine, New York, NY

Runx1 Is Required for Notch-Induced Specification of Megakaryocyte Development from Early Hematopoietic Stem and Progenitor Cells

Melanie G Cornejo1*, Thomas Mercher, Ph.D2*, Joseph D. Growney, PhD1*, Jonathan Jesneck3*, Ivan Maillard, MD, PhD4, Warren S. Pear, MD, PhD5, Jon Aster, MD, PhD6 and D. Gary Gilliland, MD, PhD1

1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA
2INSERM EMI0210, Universite Paris V-Descartes, Paris, France
3Dana Farber Cancer Institute, Boston, MA
4Life Sciences Institute Rm 6382A, Center for Stem Cell Biology, Ann Arbor, MI
5University of Pennsylvania, Philadelphia, PA
6Brigham and Women's Hosp., Boston, MA

Identification and Characterization of a Hematopoietic Stem Cell Niche in Spleen

Takuo Mizukami, Ph.D1*, Isao Hamaguchi, MD, Ph.D1, Kazuya Takizawa, Ph.D1*, Madoka Kuramitsu, Ph.D1*, Haruka Momose, Ph.D1*, Seishiro Naito, Ph.D1*, Atsuko Masumi, Ph.D1*, Seiji Okada, MD, Ph.D2* and Kazunari Yamaguchi, MD, Ph.D1*

1Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan
2Center for AIDS Research, Kumamoto University, Kumamoto, Japan

*signifies non-member of ASH