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501.P1.59 Hematopoietic Stem and Progenitor Biology Poster I

Saturday, December 6, 2008: 5:30 PM-7:30 PM
Hall A (Moscone Center)
Cannabinoid Receptors CB1 and CB2 Regulate Mobilization of Hematopoietic Stem and Progenitor CellsClinically Relevant Abstract

Hava Avraham, PhD*, Radoslaw Zagozdzon, MD, PhD*, Yigong Fu, MS*, Jerome E. Groopman, MD and Shuxian Jiang, PhD*

Division of Experimental Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA

VEGF and AMD3100-Induced Rapid Mobilization of C-Kit/Sca-1 Positive Murine Bone Marrow Cells and of Gr-1+/CD-11b+ Myeloid Cells Is Impaired in Urokinase (uPA) and Urokinase Receptor (uPAR) Deficient Mice

Mohammad Dehghani1*, Damla Olcaydu2*, Pavel Uhrin1*, Bernd Binder, MD1 and Johannes Breuss1*

1Department of Vascular Biology and Thrombosis Research, Medical University of Vienna, Vienna, Austria
2Center for Molecular Medicine, Austrian Academy of Sciences, Vienna, Austria

AMD3100 and Granulocyte Colony Stimulating Factor (G-CSF) Mobilize Different CD34+ Cell Populations Based on Global Gene and MicroRNA Expression

Robert E. Donahue, VMD1, Ping Jin, PhD2*, Aylin C. Bonifacino1*, Mark E. Metzger1* and David Stroncek, MD2*

1Hematology Branch, NHLBI, NIH, Rockville, MD
2Department of Transfusion Medicine, Clinical Center, NIH, Bethesda, MD

Hematopoietic Activity of Human Short Term Repopulating Cells in Mobilized Peripheral Blood Cell Transplants Is Restricted to the First 5 Months after TransplantationClinically Relevant Abstract

Oksana Zavidij*, Claudia R Ball, PhD*, Sylvia Fessler*, Manfred Schmidt, PhD*, Christof von Kalle, MD* and Hanno Glimm, MD

Translational Oncology, National Center for Tumor Diseases (NCT) / German Cancer Research Center (DKFZ), Heidelberg, Germany

The Sympathetic Nervous System Regulates Hematopoietic Stem and Progenitor Cell Homing and EngraftmentClinically Relevant Abstract

Daniel Lucas, PhD1* and Paul S. Frenette, M.D.2

1Department of Medicine, Mount Sinai School of Medicine, New York, NY
2Department of Medicine and Immunology Institute, Mount Sinai School of Medicine, New York, NY

The G Protein-Coupled Receptor cysLT1 Mediates Gi Protein-Dependent Chemotaxis and Adhesion of CD34+ Cells in Vitro Similar to CXCR4, but Not Progenitor Mobilization in Vivo

Adriana Drost, PhD*, Lena Jaggy*, Lothar Kanz, MD and Robert Möhle, MD

Dept. of Medicine II, University of Tübingen, Tübingen, Germany

How Can Hematopoietic Stem Cells and Myeloid Progenitors Become Transformed?

Mary Mohrin1*, Emer Bourke, PhD2*, Ciaran Morrison, PhD2* and Emmanuelle Passegue, PhD1

1Institute for Regeneration Medicine, University of California School of Medicine - San Francisco, San Francisco, CA
2Department of Biochemistry, National University of Ireland, Galway, Galway, Ireland

Hedgehog Signaling Regulates HSC Proliferation

Akil Merchant, MD, Giselle Joseph, BS* and William Matsui, MD*

Oncology, Johns Hopkins University, Baltimore, MD

Hedgehog Signaling Is Dispensable for Adult Murine Hematopoiesis and Leukemogenesis

Inga Hofmann Zhang, MD1*, Elizabeth H. Stover1*, Dana E. Cullen1*, Junhao Mao, PhD2*, Kelly J. Morgan, PhD1*, Benjamin H. Lee, MD, PhD1, Michael G. Kharas, PhD1*, Peter G. Miller1*, Rachel Okabe1*, Scott A. Armstrong, MD, PhD3, Nico Ghilardi, PhD4*, Stephen Gould, PhD4*, Fred J. deSauvage, PhD4*, Andrew P. McMahon, PhD2* and D. Gary Gilliland, MD, PhD1

1Department of Medicine, Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA
2Department of Molecular and Cellular Harvard University, Cambridge, MA
3Children's Hospital, Boston, MA
4Genentech Inc., South San Francisco, CA

Wnt/β-catenin and Pten/Akt Signaling Interaction Drives Hematopoietic Stem Cell Self-Renewal and Leukemogenesis

John M. Perry, Ph.D.*, Justin C. Grindley, Ph.D.*, Xi He, MD and Linheng Li, Ph.D.*

Stowers Institute for Medical Research, Kansas City, MO

The Neuropeptides Orexin a and B Have An Impact on Functional Properties of Human CD34+ Stem and Progenitor Cells

Ingmar Bruns, MD, PhD1, Patrick Cadeddu1*, Sebastian Büst1*, Boris Goerg2*, Johannes C Fischer3*, Oliver Selbach4*, Ulrich Steidl, MD, PhD5, Ralf Kronenwett1*, Rainer Haas1*, Guido Kobbe1* and Akos Czibere, MD, PhD1

1Hem./Onc. & Clin. Immuno., University of Düsseldorf, Dusseldorf, Germany
2Department of Gatroenterology, Hepatology and Infectiology, University of Düsseldorf, Düsseldorf, Germany
3Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine University, Düsseldorf, Germany
4Institute of Neuro- und Sensory Physiology, University of Düsseldorf, Düsseldorf, Germany
5Department of Cell Biology and Albert Einstein Cancer Center, Albert Einstein College of Medicine, New York, NY

SIRT1 Deficiency Suppresses the Maintenance of Hematopoietic Stem Cell Pool

Sachiko Ezoe*, Itaru Matsumura, Hirokazu Tanaka*, Yusuke Satoh*, Takafumi Yokota*, Kenji Oritani* and Yuzuru Kanakura*

Dept. of Hem./Onc., Osaka Univ., Suita, Japan

The RNA Editing Enzyme ADAR1 Is Required for Survival of Differentiating Hematopoietic Progenitor Cells in Adult Mice

Feng Xu, MD1*, Qingde Wang, MD., PhD.1*, Hongmei Shen, PhD2, Hui Yu, MD, PhD1*, Yanxin Li, PhD1* and Tao Cheng, MD1

1University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA
2Thomas E. Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA

Groucho/TLE Corepressors Regulate Myeloid Differentiation and Size of the Stem Cell/Progenitor PopulationClinically Relevant Abstract

Jianfeng Wang, MD, PhD1*, Farshid Dayyani, MD, PhD2*, Christopher Brynczka1* and David A. Sweetser, MD, PhD3

1Dept. of Pediatrics, Massachusetts General Hospital, Boston, MA
2Leukemia, University of Texas MD Anderson Cancer Center, Houston, TX
3Jackson 904, Massachusetts General Hospital, Boston, MA

Constitutive TGF-β1 Deficiency Induces a Defect in Hematopoietic Stem/Progenitor Cell Compartment in Fetus and Neonate

Claude Capron1*, Catherine Lacout2*, Yann Lecluse2*, Valérie Jalbert3*, Elisabeth Cramer Bordé4*, Anne Galy2*, Sabine Charrier2* and William Vainchenker2

1INSERM U790, IFR 54, Institut Gustave Roussy and U567 Institut Cochin, Villejuif:Paris, France
2INSERM U790, Institut Gustave Roussy, Villejuif, France
3Hopital Ambroise Paré, Université Versaille Pris Ile de France Ouest, Boulogne Billancourt, France
4Departement d'hématologie, Institut Cochin, Inserm, U567, Paris, France

Accelerated Repair of Vascular Injury in Diabetes by TGF-β1 Modified Hematopoietic Stem Cells (HSC)

Stephen Bartelmez, PhD1*, Francis W Ruscetti, PhD2, Patrick Iversen, PhD3*, Ashay Bhatwadekar, PhD4* and Maria Grant, MD4*

1Stem Cells, BetaStem Therapeutics, San Francisco, CA
2Laboratory of Experimental Immunology, NIH/NCI, Frederick, MD
3Biologicals, AVI-BioPharma, Corvallis, OR
4Pharmacology, University of Florida, Gainesville, FL

Bone Morphogenetic Protein 4 Regulates Hematopoietic Stem Cell Maintenance in Vivo

Devorah C. Goldman, PhD1*, Alexis S. Bailey1*, Dana L. Pfaffle1*, Jan L. Christian, PhD2* and William H. Fleming, MD, PhD1

1Department of Medicine, Division of Hematology and Medical Oncology, Oregon Health & Science University, Portland, OR
2Department of Cell and Developmental Biology, Oregon Health & Science University, Portland, OR

Latexin Regulates Stem Cell Numbers Via a NF-κB-Dependent Pathway

Ailing Li, PhD, Ying Liang*, Yi Liu* and Gary VanZant*

Internal Med., Univ. of Kentucky, Lexington, KY

Targeted Disruption of Zfp36l2, Encoding a CCCH Tandem Zinc Finger RNA-Binding Protein, Results in Defective Hematopoiesis

Deborah J. Stumpo, PhD1*, Hal E. Broxmeyer, PhD2, Scott Cooper2*, Giao Hangoc, DVM, PhD3, Peter D. Aplan, MD4, Yang Jo Chung, PhD4, Mervin C. Yoder, MD5, William C. Shelley5* and Perry J. Blackshear1*

1Laboratory of Signal Transduction, NIEHS, Research Triangle Park, NC
2Indiana University School of Medicine, Walther Oncology Center, Indianapolis, IN
3Walther Oncology Center, Indiana Univ. School of Med., Indianapolis, IN
4Genetics Branch, NIH, NCI, CCR, Bethesda, MD
5Cancer Research Institute, Wells Ctr. for Pediatric Rsch., Indianapolis, IN

The Role of Gi Signaling in Hematopoiesis

Marie Terpager1*, Hiroshi Kataoka2*, Ivo Cornelissen1*, Justin Hamilton3* and Shaun R Coughlin4*

1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA
2Lab. for Stem Cell Biology, RIKEN Center for Developmental Biology, Kobe, Japan
3Australian Centre for Blood Diseases, Monash University,, Melbourne, Australia
4Cvri, University of California, San Francisco, San Francisco, CA

The Ski oncoprotein regulates Hematopoietic Stem Cell Fitness and Functions as a Corepressor for Gfi1

Chinavenmeni S. Velu, PhD1, Michael Berk2*, Haiming Xu3*, Tristan Bourdeau, MS4*, Avedis Kazanjian4*, David A. Williams, MD5, Clemencia Colmenares, Ph.D.6* and H. Leighton Grimes, PhD4

1Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH
2Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic,, Cleveland, OH
3Experimental Hematology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH
4Cincinnati Children's Hospital Medical Center, Cincinnati, OH
5Chief, Div. of Hematology/Oncology, Children's Hospital Boston, Leland Fikes, Boston, MA
6Cancer Biology, Cleveland Clinic, Cleveland, OH

Cdx4 Is Dispensable for Murine Hematopoiesis and MLL-AF9 Mediated Leukemogenesis

Sumin Koo1*, Thomas Mercher, Ph.D2*, Shannon Mckinney-Freeman, Ph.D3*, Yuan Wang, Ph.D3*, Brian Ball4*, Brian JP Huntly, MD, PhD5*, Dimple Bansal, Ph.D4*, George Q. Daley, MD, PhD3 and D. Gary Gilliland4

1Harvard Medical School, Boston, MA
2Division of Hematology, Department of Medicine, Brigham and Women's Hospital, INSERM EMI0210, Universite Paris V-Descartes, Paris, MA, France
3Children's Hospital Boston, Boston, MA
4Brigham and Women's Hospital, Boston, MA
5Hematology, University of Cambridge, Cambridge, United Kingdom

p16INK4a Is a Key Downstream Mediator of the Deleterious Effects of FoxO Deficiency on Maintenance of the Hematopoietic Stem Cell Compartment

Zuzana Tothova1*, Stephen M Sykes1*, Dena S Leeman1*, James W Horner2*, Norman Sharpless3, Ronald A DePinho2* and D. Gary Gilliland1

1Division of Hematology, Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA
2Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA
3Lineberger Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC

DNMT3b's Role in Hematopoietic Stem Cell Self-Renewal

Matthew J Boyer*, Feng Xu, MD*, Hui Yu, MD, PhD* and Tao Cheng, MD

University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA

Deletion of Fak in Hematopoietic Stem Cells Leads to Enhanced Engraftment

Jiayun Lu1*, Cesar Nombela-Arrieta1*, Shin-Young Park1*, Yi Le1*, Li Chai2* and Leslie E. Silberstein1

1Joint Program in Transfusion Medicine, Children's Hospital Boston, Boston, MA
2Joint Program in Transfusion Medicine, Brigham and Women's Hospital, Boston, MA

Intercommunication Between C-Myc and PTEN in the Regulation of Hematopoietic Stem Cell-Niche Interactions and Hematopoietic Progenitor Cell Lineage Commitment

Yinshi Guo, Ph., D., M.D.1*, Chao Niu, MD, PhD2, Peter Breslin, PhD3*, Shubin Zhang4*, Wei Wei1*, Minghui Tang, Ph., D., M.D.1* and Jiwang Zhang, MD, PhD1*

1Oncology Institute, Loyola University Medical Center, Maywood, IL
2St. Jude Children's Hospital, Memphis, TN
3Oncology Institute, Loyola Universtiy Medical Center, Maywood, IL
4Oncology, Loyola University Medical Center, Maywood, IL

RASSF8 Overexpression Expands KLS Cells in Vitro and Blocks Murine Hematopoietic Stem Cells in a Primitive Phenotype

Helene M. Schoemans, MD*, Rikkert Snoeckx*, Shannon Buckley*, Elda Mimiola*, Sarah Schouteden* and Catherine M. Verfaillie, MD

Stem Cell Institute, UZ Leuven, Leuven, Belgium

Wiskott-Aldrich Syndrome Protein (WASP) Is An Effector of Kit Signaling

Maheswaran Mani, PhD1*, Shivkumar Venkatasubrahmanyam, PhD2*, Mrinmoy Sanyal, PhD3, Yujun Yang1*, Jing Huang1*, Shoshana Levy, PhD3*, Atul Butte, MD, PhD2*, Kenneth I. Weinberg, MD1 and Thomas Jahn, MD1

1Pediatric Stem Cell Transplantation, Stanford University School of Medicine, Stanford, CA
2Center for Biomedical Informatics Research, Stanford University School of Medicine, Stanford, CA
3Medicine, Division of Oncology, Stanford University School of Medicine, Stanford, CA

GATA-2 Regulates Granulocyte-Macrophage Progenitor Cell Function

Neil P Rodrigues, D.Phil1*, Ashleigh S Boyd, D.Phil1*, Cristina Fugazza, Ph.D1*, Gillian E May, Ph.D1*, YanPing Guo1*, Alex J Tipping, Ph.D1*, David T. Scadden, MD2, Paresh Vyas, MD, DPhil1* and Tariq Enver, Ph.D1*

1MRC Haematology Unit, University of Oxford, Headington, United Kingdom
2MGH Center for Regenerative Medicine, Harvard Stem Cell Institute, Boston, MA

Impaired Erythropoiesis in LYL-1 Deficient Mice

Claude Capron1*, Catherine Lacout2*, Yann Lecluse2*, Orianne Wagner-Ballon2*, Anna Lila Kaushik2*, Elisabeth Cramer Bordé3*, Fred Sablitzky4*, Dominique Duménil3* and William Vainchenker2

1INSERM U790, IFR 54, Institut Gustave Roussy and U567, Institut Cochin, Villejuif/Paris, France
2INSERM U790, Institut Gustave Roussy, Villejuif, France
3Departement d'hématologie, Institut Cochin, Inserm, U567, Paris, France
4Institute of Genetics, University of Nottingham, School of Biology, Nottingham, United Kingdom

*signifies non-member of ASH